Conditioning a current source using OCCII-GIC for EIT systems

Multi-Frequency EIT has been developed to evaluate the permittivity spectrum of a medium using an improved Howland source. Stray capacitance reduces the output impedance, bandwidth of the data collection leading to erroneous measurements. To address this, we have designed a current source, which uses a novel OCCII - GIC to cancel stray capacitance. The system achieves high output impedance over a 5 MHz frequency range

The Drosophila blood-brain barrier: development and function of a glial endothelium

The efficacy of neuronal function requires a well-balanced extracellular ion homeostasis and a steady supply with nutrients and metabolites. Therefore, all organisms equipped with a complex nervous system developed a so-called blood-brain barrier, protecting it from an uncontrolled entry of solutes, metabolites or pathogens. In higher vertebrates, this diffusion barrier is established by polarized endothelial cells that form extensive tight junctions, whereas in lower vertebrates and invertebrates the blood-brain barrier is exclusively formed by glial cells. Here, we review the development and function of the glial blood-brain barrier of Drosophila melanogaster. In the Drosophila nervous system, at least seven morphologically distinct glial cell classes can be distinguished. Two of these glial classes form the blood-brain barrier. Perineurial glial cells participate in nutrient uptake and establish a first diffusion barrier. The subperineurial glial cells form septate junctions, which block paracellular diffusion and thus seal the nervous system from the hemolymph. We summarize the molecular basis of septate junction formation and address the different transport systems expressed by the blood-brain barrier forming glial cells

Hybrid magnetization dynamics in Cu2OSeO3/NiFe heterostructures

We investigate the coupled magnetization dynamics in heterostructures of a single crystal of the chiral magnet Cu 2 OSeO 3 (CSO) and a polycrystalline ferromagnet NiFe (Py) thin film using broadband ferromagnetic resonance (FMR) at cryogenic temperatures. We observe the excitation of a hybrid mode (HM) below the helimagnetic transition temperature of CSO. This HM is attributed to the spin dynamics at the CSO/Py interface. We study the HM by measuring its resonance frequencies for in plane rotations of the external magnetic field. We find that the HM exhibits dominantly fourfold anisotropy in contrast to the FMR of CSO and P

Lack of the Sodium-Driven Chloride Bicarbonate Exchanger NCBE Impairs Visual Function in the Mouse Retina

Regulation of ion and pH homeostasis is essential for normal neuronal function. The sodium-driven chloride bicarbonate exchanger NCBE (Slc4a10), a member of the SLC4 family of bicarbonate transporters, uses the transmembrane gradient of sodium to drive cellular net uptake of bicarbonate and to extrude chloride, thereby modulating both intracellular pH (pHi) and chloride concentration ([Cl-]i) in neurons. Here we show that NCBE is strongly expressed in the retina. As GABAA receptors conduct both chloride and bicarbonate, we hypothesized that NCBE may be relevant for GABAergic transmission in the retina. Importantly, we found a differential expression of NCBE in bipolar cells: whereas NCBE was expressed on ON and OFF bipolar cell axon terminals, it only localized to dendrites of OFF bipolar cells. On these compartments, NCBE colocalized with the main neuronal chloride extruder KCC2, which renders GABA hyperpolarizing. NCBE was also expressed in starburst amacrine cells, but was absent from neurons known to depolarize in response to GABA, like horizontal cells. Mice lacking NCBE showed decreased visual acuity and contrast sensitivity in behavioral experiments and smaller b-wave amplitudes and longer latencies in electroretinograms. Ganglion cells from NCBE-deficient mice also showed altered temporal response properties. In summary, our data suggest that NCBE may serve to maintain intracellular chloride and bicarbonate concentration in retinal neurons. Consequently, lack of NCBE in the retina may result in changes in pHi regulation and chloride-dependent inhibition, leading to altered signal transmission and impaired visual function

A transatlantic symposium on the Restatement (Fourth)

No abstract available

Tunable Cooperativity in Coupled Spin--Cavity Systems

We experimentally study the tunability of the cooperativity in coupled spin--cavity systems by changing the magnetic state of the spin system via an external control parameter. As model system, we use the skyrmion host material Cu$_2$OSeO$_3$ coupled to a microwave cavity resonator. In the different magnetic phases we measure a dispersive coupling between the resonator and the magnon modes and model our results by using the input--output formalism. Our results show a strong tunability of the normalized coupling rate by magnetic field, allowing us to change the magnon--photon cooperativity from 1 to 60 at the phase boundaries of the skyrmion lattice state

Quality control and error assessment of the Aeolus L2B wind results from the Joint Aeolus Tropical Atlantic Campaign

Since the start of the European Space Agency's Aeolus mission in 2018, various studies were dedicated to the evaluation of its wind data quality and particularly to the determination of the systematic and random errors in the Rayleigh-clear and Mie-cloudy wind results provided in the Aeolus Level-2B (L2B) product. The quality control (QC) schemes applied in the analyses mostly rely on the estimated error (EE), reported in the L2B data, using different and often subjectively chosen thresholds for rejecting data outliers, thus hampering the comparability of different validation studies. This work gives insight into the calculation of the EE for the two receiver channels and reveals its limitations as a measure of the actual wind error due to its spatial and temporal variability. It is demonstrated that a precise error assessment of the Aeolus winds necessitates a careful statistical analysis, including a rigorous screening for gross errors to be compliant with the error definitions formulated in the Aeolus mission requirements. To this end, the modified Z score and normal quantile plots are shown to be useful statistical tools for effectively eliminating gross errors and for evaluating the normality of the wind error distribution in dependence on the applied QC scheme, respectively. The influence of different QC approaches and thresholds on key statistical parameters is discussed in the context of the Joint Aeolus Tropical Atlantic Campaign (JATAC), which was conducted in Cabo Verde in September 2021. Aeolus winds are compared against model background data from the European Centre for Medium-Range Weather Forecasts (ECMWF) before the assimilation of Aeolus winds and against wind data measured with the 2 µm heterodyne detection Doppler wind lidar (DWL) aboard the Falcon aircraft. The two studies make evident that the error distribution of the Mie-cloudy winds is strongly skewed with a preponderance of positively biased wind results distorting the statistics if not filtered out properly. Effective outlier removal is accomplished by applying a two-step QC based on the EE and the modified Z score, thereby ensuring an error distribution with a high degree of normality while retaining a large portion of wind results from the original dataset. After the utilization of the described QC approach, the systematic errors in the L2B Rayleigh-clear and Mie-cloudy winds are determined to be below 0.3 m s−1 with respect to both the ECMWF model background and the 2 µm DWL. Differences in the random errors relative to the two reference datasets (Mie vs. model is 5.3 m s−1, Mie vs. DWL is 4.1 m s−1, Rayleigh vs. model is 7.8 m s−1, and Rayleigh vs. DWL is 8.2 m s−1) are elaborated in the text.</p