Wire grip and motion score for TBI mice.

<p>Mice are assessed the score for the best level they reach.</p

Neurological Severity Score (NSS) for TBI Mice.

<p>Mice are awarded 1 point for each failure to complete a task.</p

Fluoro Jade C staining of lesion areas in brain sections.

<p>All sections were taken from mice sacrificed at 14 days post-TBI. Green is FJC and blue is DAPI )control for equal numbers of cell nuclei. (A) Sham-TBI control mouse; (B) real-TBI sham Tx mouse. (C) TBI mouse treated with 1 laser Tx. (C) TBI mouse treated with 3 laser Tx (E) TBI mouse treated with 14 laser Tx (F) Mean green staining calculated with Image J from mice (n = 2–3) in the groups in panels A–E. # P<0.05 vs TBI sham; * P<0.05 vs 14 laser Tx. One way ANOVA.</p

WGMT scores of the mice.

<p>Mean (n = 8–14) WGMT scores measured over 4 weeks, of mice in 9 groups consisting of sham TBI mice, sham control mice, 810-nm laser treated TBI mice (18 J/cm² delivered at 25 mW/cm²). (A) Mice and controls given 1 laser Tx or 1 sham Tx at 4 hours post TBI; (B) Mice and controls given 3 daily laser Tx or 3 sham Tx; (C) Mice and controls given 14 daily laser Tx or 14 sham Tx. (D) Mean areas under curve of WGMT time courses from mice in different groups. P values given were determined by one-way ANOVA.</p

BrdU staining of lesion areas in brain sections.

<p>All sections were taken from mice sacrificed at 28 days post-TBI. Red is BrdU and blue is DAPI (control for equal numbers of cell nuclei). (A) Sham control mouse; (B) TBI sham untreated mouse. (C) TBI mouse treated with 1 laser Tx. (C) TBI mouse treated with 3 laser Tx (E) TBI mouse treated with 14 laser Tx (F) Mean (n = 6) red staining calculated with Image J from mice (n = 6) in the groups in panels A–E. *** P<0.001 vs TBI sham; # P<0.05 vs 1 laser Tx; <a href="mailto:@@" target="_blank">@@</a> P<0.01 vs 14 laser Tx. One way ANOVA.</p

Transmittance of laser power through ex vivo mouse scalp and skull (average ± SD, n = 3).

<p>Transmittance of laser power through ex vivo mouse scalp and skull (average ± SD, n = 3).</p

Forced swim test for depression.

<p>Immobility duration in forced swim test (FST) at (A) 1 day and (B) 28 days after brain injury and laser irradiation. Values are in mean ± S.E.M. (n = 10). ‡ <i>P</i><0.05 vs. values at 1 day after TBI. * <i>P</i><0.05 between the groups.</p

Area of brain lesions.

<p>Results of quantitative analysis of the area of cavitary cortical lesions in brain tissue on the basis of the histological images. Values are means ± S.E.M. (n = 9). * <i>P</i><0.05.</p

NSS scores after laser treatment.

<p>(A) Time course of neurological severity score (NSS) of mice with TBI receiving either control (no laser-treatment), or 810-nm laser (36 J/cm² delivered at 50 mW/cm² with a spot size of 0.78 cm²) in either CW, PW 10 Hz or PW 100 Hz modes. Results are expressed as mean ± S.E.M (n = 10). *** <i>P</i><0.001 vs. the other conditions. (B) Mean areas under the NSS-time curves in the two-dimensional coordinate system over the 28-day study for the 4 groups of mice. Results are means ± SD (n = 10).</p

Neurological severity score (NSS) for mice.

<p>For each failed task the mouse receives 1 point. Maximum = 10 (failure in all tasks), minimum = 0 (success in all tasks).</p