1 research outputs found
Noninvasive Multiplexed Analysis of Bladder Cancer-Derived Urine Exosomes via Janus Magnetic Microspheres
Bladder cancer greatly endangers human health, and its
early diagnosis
is of vital importance. Exosomes, which contain proteins and nucleic
acids related to their source cells, are expected to be an emerging
biomarker for bladder cancer detection. Here, we propose a novel system
for multiplexed analysis of bladder cancer-derived urine exosomes
based on Janus magnetic microspheres as barcoded microcarriers. The
microcarriers are constructed by droplet-templated coassembly of colloidal
silica nanoparticles and magnetic nanoparticles under a magnetic field.
The microcarriers possess one hemisphere with structural color and
the other hemisphere with magneto-responsiveness. Benefiting from
the unique structure, these Janus microcarriers could serve as barcodes
and could move controllably in a sample solution, thus realizing the
multiplex detection of exosomes with high sensitivity. Notably, the
present platform is noninvasive since a urine specimen, as an ideal
source of bladder cancer-derived exosomes, is employed as the sample
solution. This feature, together with the good sensitivity, specificity,
low sample consumption, and easy operation, indicates the great potential
of the platform for bladder cancer diagnosis in clinical applications