ChordMics: Acoustic Signal Purification with Distributed Microphones

Acoustic signal acts as an essential input to many systems. However, the pure acoustic signal is very difficult to extract, especially in noisy environments. Existing beamforming systems are able to extract the signal transmitted from certain directions. However, since microphones are centrally deployed, these systems have limited coverage and low spatial resolution. We overcome the above limitations and present ChordMics, a distributed beamforming system. By leveraging the spatial diversity of the distributed microphones, ChordMics is able to extract the acoustic signal from arbitrary points. To realize such a system, we further address the fundamental challenge in distributed beamforming: aligning the signals captured by distributed and unsynchronized microphones. We implement ChordMics and evaluate its performance under both LOS and NLOS scenarios. The evaluation results tell that ChordMics can deliver higher SINR than the centralized microphone array. The average performance gain is up to 15dB

A Developmentally Regulated Splice Variant from the Complexlola Locus Encoding Multiple Different Zinc Finger Domain Proteins Interacts with the Chromosomal Kinase JIL-1

Using a yeast two-hybrid screen we have identified a novel isoform of the lola locus, Lola zf5, that interacts with the chromosomal kinase JIL-1. We characterized thelolalocus and provide evidence that it is a complex locus from which at least 17 different splice variants are likely to be generated. Fifteen of these each have a different zinc finger domain, whereas two are without. This potential for expression of multiple gene products suggests that they serve diverse functional roles in different developmental contexts. By Northern and Western blot analyses we demonstrate that the expression of Lola zf5 is developmentally regulated and that it is restricted to early embryogenesis. Immunocytochemical labeling with a Lola zf5-specific antibody of Drosophila embryos indicates that Lola zf5 is localized to nuclei. Furthermore, by creating double-mutant flies we show that a reduction of Lola protein levels resulting from mutations in the lola locus acts as a dominant modifier of a hypomorphic JIL-1allele leading to an increase in embryonic viability. Thus, genetic interaction assays provide direct evidence that gene products from the lola locus function within the same pathway as the chromosomal kinase JIL-1

Lack of spontaneous ocular neovascularization and attenuated laser-induced choroidal neovascularization in IGF-I overexpression transgenic mice

Robust IGF-I overexpression induces ocular angiogenesis in mice. To investigate the effect of subtle IGF-I overexpression, we examined the ocular phenotype of IGF-II promoter-driven IGF-I transgenic mice. Despite 2.5-fold elevation of IGF-I mRNA in the retina and 29 and 52% increase of IGF-I protein in the retina and aqueous humor, respectively, no ocular abnormality was observed in these transgenics. This was correlated with unaltered VEGF mRNA levels in the transgenic retina. The transgene was also associated with an attenuated laser-induced choroidal neovascularization. Differential expression levels and pattern of IGF-I gene may underlie the different retinal phenotypes in different transgenic lines

Porphysome nanovesicles generated by porphyrin bilayers for use as multimodal biophotonic contrast agents

Optically active nanomaterials promise to advance a range of biophotonic techniques through nanoscale optical effects and integration of multiple imaging and therapeutic modalities. Here, we report the development of porphysomes; nanovesicles formed from self-assembled porphyrin bilayers that generated large, tunable extinction coefficients, structure-dependent fluorescence self-quenching and unique photothermal and photoacoustic properties. Porphysomes enabled the sensitive visualization of lymphatic systems using photoacoustic tomography. Near-infrared fluorescence generation could be restored on dissociation, creating opportunities for low-background fluorescence imaging. As a result of their organic nature, porphysomes were enzymatically biodegradable and induced minimal acute toxicity in mice with intravenous doses of 1,000 mg kg^(−1). In a similar manner to liposomes, the large aqueous core of porphysomes could be passively or actively loaded. Following systemic administration, porphysomes accumulated in tumours of xenograft-bearing mice and laser irradiation induced photothermal tumour ablation. The optical properties and biocompatibility of porphysomes demonstrate the multimodal potential of organic nanoparticles for biophotonic imaging and therapy

Grain quality evaluations of hybrids between Triticum aestivum and Sorghum bicolor produced by pollen tube pathway method

Abstract Distant hybridization makes it possible to transfer the genome of one species to another, and the pollen tube pathway method has been widely used based on the hypothesis that such distant hybridization process provided the opportunity of the recombination of DNA segment. In this study, we carried out a distant hybridization between Triticum aestivum and Sorghum bicolor via pollen tube pathway method, and five new wheat lines including 9122 (S. bicolor 2D + T. aestivum L13), 9141 (S. bicolor 5D + T. aestivum L10), 9144 (S. bicolor 2D + T. aestivum G8), 9145 (S. bicolor 5D + T. aestivum G8) and 0154 (S. bicolor 10D + T. aestivum G8) were selected to determine the grain quality characteristics of these hybrids. Therefore, the properties of their flour mixogram and high molecular weight subunit of hybrids were analyzed. The variations of mixogram peak time, mixogram peak height, mixogram height in the seventh minute and sedimentation value in the five hybrids were observed, while mixogram width at the seventh minute of all the hybrids was improved. The mutation of high molecular weight subunits of glutenin happened including the composition pattern and content of HMW-GS. In hybrid 9144, the high molecular weight glutenin subunits 5+10 were presented instead of subunits 2+12 of its maternal parent G8. The results indicated that distant hybridization between T. aestivum and S. bicolor via pollen tube pathway method improved the wheat grain quality, which will provide more opportunities for the selection of new wheat cultivar with improving grain quality

The p38 MAPK pathway is essential for skeletogenesis and bone homeostasis in mice

Nearly every extracellular ligand that has been found to play a role in regulating bone biology acts, at least in part, through MAPK pathways. Nevertheless, much remains to be learned about the contribution of MAPKs to osteoblast biology in vivo. Here we report that the p38 MAPK pathway is required for normal skeletogenesis in mice, as mice with deletion of any of the MAPK pathway member–encoding genes MAPK kinase 3 (Mkk3), Mkk6, p38a, or p38b displayed profoundly reduced bone mass secondary to defective osteoblast differentiation. Among the MAPK kinase kinase (MAP3K) family, we identified TGF-β–activated kinase 1 (TAK1; also known as MAP3K7) as the critical activator upstream of p38 in osteoblasts. Osteoblast-specific deletion of Tak1 resulted in clavicular hypoplasia and delayed fontanelle fusion, a phenotype similar to the cleidocranial dysplasia observed in humans haploinsufficient for the transcription factor runt-related transcription factor 2 (Runx2). Mechanistic analysis revealed that the TAK1–MKK3/6–p38 MAPK axis phosphorylated Runx2, promoting its association with the coactivator CREB-binding protein (CBP), which was required to regulate osteoblast genetic programs. These findings reveal an in vivo function for p38β and establish that MAPK signaling is essential for bone formation in vivo. These results also suggest that selective p38β agonists may represent attractive therapeutic agents to prevent bone loss associated with osteoporosis and aging

Pathological Features of Enterovirus 71-Associated Brain and Lung Damage in Mice Based on Quantitative Proteomic Analysis

The outbreaks of enterovirus 71 (EV71)-associated hand, foot, and mouth disease (HFMD) have emerged as an emergency of global health due to its association with fatal encephalitis and subsequent neurogenic pulmonary edema; however, the molecular characteristics and pathological features underlying EV71-associated encephalitis and pulmonary edema remain largely unknown. In this study, we performed a proteomic analysis of fresh brain and lung tissues from EV71-infected mice at 7 days post infection. We detected a perturbed expression of 148 proteins in the brain and 78 proteins in the lung after EV71 expression. Further analysis showed that the dysregulated proteins in the brain are involved in a variety of fundamental biological pathways, including complement and coagulation cascades, innate and adaptive immune responses, platelet activation, and nitrogen metabolism, and those proteins in the lung participate in innate and adaptive immune responses, phagosome, arginine biosynthesis, and hypoxia-inducible factor 1 signaling pathway. Our results suggested that immune activation, complement and coagulation dysfunction, platelet activation, imbalance of nitrogen metabolism, and hypoxia could be involved in the pathogenesis of EV71, which explains the major clinical manifestation of hyperinflammatory status of severe HFMD cases. Our study provides further understanding of the molecular basis of EV71 pathogenesis