Uncertainty analysis

Results of Monte Carlo uncertainty analysis for each year's data

City level buliding operational carbon emissions

Contains data on carbon emissions from building operations and six types of end-uses in 321 Chinese cities from 2015-2020

Map of lentiviral vector.

<p>Map of lentiviral vector.</p

RT-PCR amplification of a specific fragment of the lentiviral vector after incubation with sperm cells.

<p>Lane M: DNA Ladder DL2000 (2000, 1000, 750, 500, 250 and 100 bp from top to bottom). Lane 1: Negative control with H₂O as template. Lane 2: Negative control with total RNA extracted from sperm alone as template, both of which showed no PCR products. Lanes 3–6: Specific PCR products were detected in sperm samples incubated with lentiviral particles for 2, 4, 6 and 8 h, respectively.</p

Identification of transgenic pigs by Southern blot analysis.

<p>Southern blot was performed under optimized condition with a Dig-lableled probe of 649 bp fragment. Samples in lanes 1 and 2 are from PCR-positive the founder No. 18 and No. 41, respectively, lanes 3–8 are their offsprings; The specific hybridization signal was detected in Lanes 1, 2, 4, 9; lanes 3, 5, 6, 7, 8 are negative, respectively; lane 10 is wild type control; lane 11 is positive controls (The plasmid pLV-siRNA); M is DNA Molecular Weight VII (DIG-labeled Roche).</p

Absorption of lentiviral particles by pig spermatozoa under a scanning electronic microscope and immunochemical detection of lentiviral particles on spermatozoa.

<p>A: lentiviral particles (40000×). B: Two lentiviral particles are clearly attached on the head of one spermatozoa (10000×). C: Two lentiviral particles absorbed on a spermatozoa tail (25000×). D: Observation of interaction between a lentiviral particle and the spermatozoa surface in an enlarged image (60000×). E: A lentiviral particle entering a spermatozoon (7500×). Immunuchemical detection of lentiviral particles attached on sperm was performed using a monoclonal mouse anti-VSV-Glycoprotein antibody, F was view field of porcine spermatozoa (200×), and red fluorescence was clearly observed emitted at 570 nm (G, 200×).</p

PCR amplification of transgene DNA sequences in piglets.

<p>PCR amplification of transgene DNA sequences in piglets.</p

Number of lentiviral particles associated with sperm cells after incubation (n = 3).

<p>Number of lentiviral particles associated with sperm cells after incubation (n = 3).</p