Temporal Proteomic and Phosphoproteomic Analysis of EV-A71-Infected Human Cells

Human enterovirus A71 (EV-A71), a member of the Picornaviridae family, is one of the main etiological viruses that lead to hand, foot, and mouth disease (HFMD). We utilized a multiplex tandem mass tag-based quantitative proteomic technique to monitor the alternation of the whole cell proteome and phosphoproteome of human rhabdomyosarcoma cells over the course of EV-A71 infection. We successfully quantified more than 7000 host proteins and 17,000 phosphosites, of which 80 proteins and nearly 1700 phosphosites were significantly regulated upon viral infection. We found that Myc proto-oncogene protein level decreased significantly, benefiting EV-A71 replication. Multiple signaling pathways were regulated in phosphorylation events that converge for protein translation, cell cycle control, and cell survival. Numerous host factors targeted by virus proteins are phosphoproteins. These factors are involved in host translational initiation, unfolded protein response, endoplasmic reticulum stress, and stress granule formation, and their phosphorylation may play key roles in the virus life cycle. Notably, we identified three conserved phosphorylation sites on viral polyproteins that have not been previously reported. Our study provides valuable resources for a systematic understanding of the interaction between the host cells and the EV-A71 at the protein and the post-translational level

Relationship between clinicopathological characteristics of overall survival in patients with stage IB-IIA cervical cancer and LANR.

Relationship between clinicopathological characteristics of overall survival in patients with stage IB-IIA cervical cancer and LANR.</p

Forest plots of the associations of LANR with the overall survival of stage IB-IIA cervical cancer patients in different subgroups.

Forest plots of the associations of LANR with the overall survival of stage IB-IIA cervical cancer patients in different subgroups.</p

The ROC curve for progression-free survival of Alb, Lym, Neu, and LANR.

A: Alb for PFS. B: Lym for PFS. C: Neu for PFS. D: LANR for PFS.</p

Temporal Proteomic and Phosphoproteomic Analysis of EV-A71-Infected Human Cells

Human enterovirus A71 (EV-A71), a member of the Picornaviridae family, is one of the main etiological viruses that lead to hand, foot, and mouth disease (HFMD). We utilized a multiplex tandem mass tag-based quantitative proteomic technique to monitor the alternation of the whole cell proteome and phosphoproteome of human rhabdomyosarcoma cells over the course of EV-A71 infection. We successfully quantified more than 7000 host proteins and 17,000 phosphosites, of which 80 proteins and nearly 1700 phosphosites were significantly regulated upon viral infection. We found that Myc proto-oncogene protein level decreased significantly, benefiting EV-A71 replication. Multiple signaling pathways were regulated in phosphorylation events that converge for protein translation, cell cycle control, and cell survival. Numerous host factors targeted by virus proteins are phosphoproteins. These factors are involved in host translational initiation, unfolded protein response, endoplasmic reticulum stress, and stress granule formation, and their phosphorylation may play key roles in the virus life cycle. Notably, we identified three conserved phosphorylation sites on viral polyproteins that have not been previously reported. Our study provides valuable resources for a systematic understanding of the interaction between the host cells and the EV-A71 at the protein and the post-translational level

The Kaplan–Meier curves for overall survival of cervical cancer patients based on LANR.

The Kaplan–Meier curves for overall survival of cervical cancer patients based on LANR.</p

The ROC curve for overall survival of Alb, Lym, Neu, and LANR.

A: Alb for OS. B: Lym for OS. C: Neu for OS. D: LANR for OS.</p

Risk factors for PFS in CC patients with stage IB-IIA by univariate and multiple Cox regression analysis.

Risk factors for PFS in CC patients with stage IB-IIA by univariate and multiple Cox regression analysis.</p

Clinicopathological Characteristics of 202 Patients with stage IB-IIA Cervice Cancer.

Clinicopathological Characteristics of 202 Patients with stage IB-IIA Cervice Cancer.</p

Risk factors for OS in CC patients with stage IB-IIA by univariate and multiple Cox regression analysis.

Risk factors for OS in CC patients with stage IB-IIA by univariate and multiple Cox regression analysis.</p