4 research outputs found
<i>N</i>,<i>N</i>âDimethylformamide (DMF) as a Source of Oxygen To Access αâHydroxy Arones via the αâHydroxylation of Arones
An unprecedented α-hydroxylation
strategy was developed for
the synthesis of α-hydroxy arones using <i>N</i>,<i>N</i>-dimethylformamide (DMF) as an oxygen source. Control experiments
demonstrated that the oxygen atom of the hydroxy group in the α-hydroxy
arones produced in this reaction was derived from DMF. This new reaction
therefore not only provides an alternative strategy for the α-hydroxylation
of arones but also highlights the possibility of using the inexpensive
common solvent DMF as a source of oxygen in organic synthesis
Preparation of 1,2-Oxazetidines from Styrenes and Arylamines via a Peroxide-Mediated [2 + 1 + 1] Cycloaddition Reaction
The
first regioselective synthesis of polysubstituted 1,2-oxazetidines,
based on the [2 + 1 + 1] radical tandem cycloaddition of styrenes,
arylamines, and <i>tert</i>-butyl hydroperoxide (TBHP),
is described. This simple and direct method allows the synthesis of
1,2-oxazetidines with a broad substrate scope from easily accessible
materials. TBHP was employed in this conversion not only as the oxidant
but also as the source of âOâ for the carbonyl group
in the products
Nitrilase-Activatable Noncanonical Amino Acid Precursors for Cell-Selective Metabolic Labeling of Proteomes
Cell-selective
protein metabolic labeling is of great interest
for studying cellâcell communications and tissue homeostasis.
We herein describe a nitrilase-activatable noncanonical amino acid
tagging (NANCAT) strategy that exploits an exogenous nitrilase to
enzymatically convert the nitrile-substituted precursors to their
corresponding noncanonical amino acids (ncAAs), l-azidohomoalanine
(Aha) or homopropargylglycine (Hpg), in living cells. Only cells expressing
the nitrilase can generate Aha or Hpg <i>in cellulo</i> and
metabolically incorporate them into the nascent proteins. Subsequent
click-labeling of the azide- or alkyne-incorporated proteins with
fluorescent probes or with affinity tags enables visualization and
proteomic profiling of nascent proteomes, respectively. We have demonstrated
that NANCAT can serve as a versatile strategy for cell-selective labeling
of proteomes in both bacterial and mammalian cells
Organosilane with Gemini-Type Structure as the Mesoporogen for the Synthesis of the Hierarchical Porous ZSMâ5 Zeolite
A new kind of organosilane (1,6-bisÂ(diethylÂ(3-trimethoxysilylpropyl)Âammonium)
hexane bromide) with a gemini-type structure was prepared and used
as a mesoporogen for the synthesis of hierarchical porous ZSM-5 zeolite.
There are two quaternary ammonium centers along with double-hydrolyzable
âRSiÂ(OMe)<sub>3</sub> fragments in the organosilane, which
results in a strong interaction between this mesoporogen and silicaâalumina
gel. The organosilane can be easily incorporated into the ZSM-5 zeolite
structure during the crystallization process, and it was finally removed
by calcination, leading to secondary pores in ZSM-5. The synthesized
ZSM-5 has been systematically studied by XRD, nitrogen adsorption,
SEM, TEM, TG, and solid-state one-dimensional (1D) and two-dimensional
(2D) NMR, which reveal information on its detailed structure. It has
a hierarchical porosity system, which combines the intrinsic micropores
coming from the crystalline structure and irregular mesopores created
by the organosilane template. Moreover, the mesoposity including pore
size and volume within ZSM-5 can be systematically tuned by changing
the organosilane/TEOS ratio, which confirms that this organosilane
has high flexibility of use as a template for the synthesis of hierarchical
porous zeolite