Representative flow cytometric analysis of the percentage of MDSCs in PBMCs in asthma patients, budesonide-treated asthma patients, healthy controls and pneumonia patients .

<p>(A) Percentage of MDSCs (CD11b⁺/CD33⁺) in PBMCs in the normal group. (B) Percentage of MDSCs in PBMCs in the attack asthma group. (C) Percentage of MDSCs in PBMCs in the budesonide treated group. (D) Percentage of MDSCs in PBMCs in the pneumonia group.</p

Different staining of the airway from normal controls (A, D, G, J, M, P, S, V), asthma mice (B, E, H, K, N, Q, T, W) and alleviated mice (C, F, I, L, O, R, U, X) and showed significant differences (Scale bars, 200 µm).

<p>(A–C), Airway H&E staining on mice from three groups shows significant difference (×200); (D–F), The PAS staining of distinct mice show significant difference; (G–I), MDSCs IHC staining on mice from three groups shows significant difference (×200), arrows indicate positive staining; (J–L), higher magnification of MDSCs IHC staining; (M–O), IL-10 IHC staining on mice from three groups shows significant difference; (P–R), IL-12 IHC staining on mice from three groups shows significant difference (×200); (S–U), TNF-α IHC staining on mice from three groups shows significant difference (×200); (V–X), IL-6 IHC staining on mice from three groups shows significant difference (×200).</p

The correlation between the levels of MDSCs and the level of IL-10 and IL-12 in asthma patients.

<p>(A) The positive correlation between the level of MDSCs and the level of serum IL-10 in asthma attack patients. (B) The negative correlation between the level of MDSCs and the level of serum IL-12 in attack asthma group.</p

The percentage of MDSCs in the PBMCs, and the level of serum IL-10 and IL-12, IgE, EOS in normal healthy controls, pneumonia patients, attack asthma patients, and alleviated patients .

#<p>compared to the normal group;</p>*<p>compared to the pneumonia group;</p>$<p>cmpared to the budesonide treated group.</p

The level of MDSCs, IL-10, and IL-12, determined by IHC staining of lung tissues from the three groups of mice .

*<p>compared with normal control group, <i>P</i><0.05;</p>#<p>Compared with asthma mice group, <i>P</i><0.05.</p