Study on the Iodine 125 Uptake of H460 Lung Cancer Cell Line by Co-transfection with the Human Sodium/Iodide Symporter and the Human Thyroperoxidase

Background and objective Lung cancer harms people’s health or even lives severely. Especially, the therapy of non-small cell lung cancer (NSCLC) has not been obviously improved for many years. The aim of this study is to transfer the human sodium/iodide symporter (hNIS) and the human thyroperoxidase (hTPO) genes into H460 lung cancer cell line, and to study the uptake ability of iodide after co-transfected hTPO and hNIS gene in cell lines. Methods Through cloning, recombination, packaging and amplifying, the recombinant adenosine virus (AdTPO) was constructed. Then the protein expression of AdTPO was tested by Western blot. After transfected hNIS gene into human lung cancer cell line H460 through liposome, stably expressing hNIS gene cell lines (hNIS-H460) selected by G418 antibiotics was determined as hNIS-H460 group. Using AdTPO, hTPO gene was transducted into hNIS-H460, as AdTPO-hNIS-H460 group. H460 cell without hNIS gene was applied as control group (H460). Then, we investigated the 125I uptake assay of the above cells. Results We were successful in co-transfecting hNIS and hTPO gene into human lung cell lines H460, and were obtained hNIS and hTPO gene lung cancer cell lines (hNIS-H460 and AdTPO-hNIS-H460). In AdTPO-hNIS-H460, hNIS-H460 and H460, the uptake ability of 125I was (59 637.67±1 281.13), (48 622.17±2 242.28) and (1 440.17±372.86) counts•min-1. The uptake ability of 125I was 41 fold higher in AdTPO-hNIS-H460 than in blank control H460 (P<0.01), and 34 fold higher in hNIS-460 than in blank control H460 (P<0.01), and 1.2 fold higher in AdTPO-hNIS-H460 than in hNIS-H460 (P<0.01). Conclusion The uptake ability of 125I could increase by co-transfected hNIS and hTPO genes into human lung cancer cell lines H460

Effects of jet-induced medium excitation in γ\gamma-hadron correlation in A+A collisions

Coupled Linear Boltzmann Transport and hydrodynamics (CoLBT-hydro) is developed for co-current and event-by-event simulations of jet transport and jet-induced medium excitation (j.i.m.e.) in high-energy heavy-ion collisions. This is made possible by a GPU parallelized (3+1)D hydrodynamics that has a source term from the energy-momentum deposition by propagating jet shower partons and provides real time update of the bulk medium evolution for subsequent jet transport. Hadron spectra in $\gamma$-jet events of A+A collisions at RHIC and LHC are calculated for the first time that include hadrons from both the modified jet and j.i.m.e.. CoLBT-hydro describes well experimental data at RHIC on the suppression of leading hadrons due to parton energy loss. It also predicts the enhancement of soft hadrons from j.i.m.e. The onset of soft hadron enhancement occurs at a constant transverse momentum due to the thermal nature of soft hadrons from j.i.m.e. which also have a significantly broadened azimuthal distribution relative to the jet direction. Soft hadrons in the $\gamma$ direction are, on the other hand, depleted due to a diffusion wake behind the jet.Comment: 4 pages, 4 figures in LaTeX, final version published in PL

Atmospheric chemistry of CH\u3csub\u3e3\u3c/sub\u3eCHO: the hydrolysis of CH\u3csub\u3e3\u3c/sub\u3eCHO catalyzed by H\u3csub\u3e2\u3c/sub\u3eSO\u3csub\u3e4\u3c/sub\u3e

Elucidating atmospheric oxidation mechanisms and the reaction kinetics of atmospheric compounds is of great importance and necessary for atmospheric modeling and the understanding of the formation of atmospheric organic aerosols. While the hydrolysis of aldehydes has been detected in the presence of sulfuric acid, the reaction mechanism and kinetics remain unclear. Herein, we use electronic structure methods with CCSD(T)/CBS accuracy and canonical variational transition state theory combined with small-curvature tunneling to study the reaction mechanism and kinetics of the hydrolysis of CH3CHO. The calculated results show that the hydrolysis of CH3CHO needs to overcome an energy barrier of 37.21 kcal mol−1, while the energy barrier is decreased to −9.79 kcal mol−1 with a sulfuric acid catalyst. In addition, the calculated kinetic results show that the H2SO4⋯H2O + CH3CHO reaction is faster than H2SO4 + CH3CHO⋯H2O. Additionally, the H2SO4⋯H2O + CH3CHO reaction can play an important role in the sink of CH3CHO below 260 K occurring during the night period when OH, H2SO4, and H2O concentrations are 104, 108, and 1017 molecules cm−3, respectively, because it can compete well with the CH3CHO + OH reaction. There are wide implications in atmospheric chemistry from these findings because of the potential importance of the catalytic effect of H2SO4 on the hydrolysis of CH3CHO in the atmosphere and in the formation of secondary organic aerosols

Containment Control of Multi-Agent Systems with Dynamic Leaders Based on a PInPI^n-Type Approach

This paper studies the containment control problem of multi-agent systems with multiple dynamic leaders in both the discrete-time domain and the continuous-time domain. The leaders' motions are described by $(n-1)$-order polynomial trajectories. This setting makes practical sense because given some critical points, the leaders' trajectories are usually planned by the polynomial interpolations. In order to drive all followers into the convex hull spanned by the leaders, a $PI^n$-type ($P$ and $I$ are short for {\it Proportion} and {\it Integration}, respectively; $I^n$ implies that the algorithm includes high-order integral terms) containment algorithm is proposed. It is theoretically proved that the $PI^n$-type containment algorithm is able to solve the containment problem of multi-agent systems where the followers are described by any order integral dynamics. Compared with the previous results on the multi-agent systems with dynamic leaders, the distinguished features of this paper are that: (1) the containment problem is studied not only in the continuous-time domain but also in the discrete-time domain while most existing results only work in the continuous-time domain; (2) to deal with the leaders with the $(n-1)$-order polynomial trajectories, existing results require the follower's dynamics to be $n$-order integral while the followers considered in this paper can be described by any-order integral; and (3) the "sign" function is not employed in the proposed algorithm, which avoids the chattering phenomenon. Furthermore, in order to illustrate the practical value of the proposed approach, an application, the containment control of multiple mobile robots is studied. Finally, two simulation examples are given to demonstrate the effectiveness of the proposed algorithm