Additional file 3: of Tranexamic acid in total shoulder arthroplasty and reverse shoulder arthroplasty: a systematic review and meta-analysis

Figure S2. Forest plot and meta-analysis of blood loss via drainage. The TXA group had less blood loss via drainage than the non-TXA group in both topical TXA and IV TXA subgroups. There was no significant subgroup difference. (data from Gillespie 2015 and Friedman 2017 were estimated from median and range). (TIFF 1562 kb

Additional file 2: of Tranexamic acid in total shoulder arthroplasty and reverse shoulder arthroplasty: a systematic review and meta-analysis

Figure S1. Forest plot and meta-analysis of Hb change. The TXA group had a lower change in Hb than the non-TXA group in both the topical TXA and IV TXA subgroups. There was no significant subgroup difference. (data from Gillespie 2015 and Friedman 2017 were estimated from median and range). (TIFF 1923 kb

Additional file 1: of Tranexamic acid in total shoulder arthroplasty and reverse shoulder arthroplasty: a systematic review and meta-analysis

Appendix 1. Database search strategy. (DOC 211 kb

Schirmer’s test measured in New Zealand rabbits (in vivo) after exposing the rabbit eye to various FA concentrations (0–600 ppm) for 5 minutes and examined at indicated time after exposure.

<p>Vertical bars denote standard deviation of the mean. [*] indicates P<0.05, [**] for P<0.01 and [***] for P<0.001 as compared to the respective control group.</p

Western blots and the bar graphs of ratios for ERK2/pERK2 and JNK/pJNK in corneal epithelial cells (a) and conjunctiva explanted from rabbit eyes (b) treated with various concentrations (0–200 ppm) of FA for 5 minutes <b><i>in vivo</i></b> and examined at 2 months after treatment.

<p>The pERK2 and pJNK indicates phosphorylated ERK2 and phosphorylated JNK, respectively. ERK2 and JNK denote total ERK2 and total JNK, respectively. Actin is used as an internal control. Vertical bar indicates standard deviation of the mean. [*] indicates P<0.05 as compared to the control (0 ppm) group.</p

Cell morphology in rabbit corneal epithelial cells exposed to various FA concentrations for 3 minutes and examined at one day (a) and seven day (b) after exposure.

<p>Grades (Gr) indicated are according to the grading system listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066649#pone-0066649-t001" target="_blank">Table 1</a>.</p

Grading system for cell morphology examined under microscope.

<p>Grading system for cell morphology examined under microscope.</p

Cell numbers of rabbit corneal epithelial cells exposed to 100 ppm for various times (0–30 min) and examined with trypan blue staining at indicated time intervals (1–7 day) after exposure.

<p>Statistical comparisons with control are indicated as [*] for P<0.05, [**] for P<0.01 and [***] for P<0.001.</p

Grading for cells treated with FMD.

<p>Grading for cells treated with FMD.</p

Representative graphs of flow cytometry assays are shown in (a) and (b).

<p>In the FITC-Annexin V/PI labeling experiment (a), the corneal epithelial cells were treated with 0, 100, 600 ppm FA for 3 min and examined 4 hours later. Apoptotic (G4 region) and necrotic (G1 and G2 regions) cells appear increased in FA treated cells as compared with the control (0 ppm). In the cell cycle examination (b), FA-treated cells increase at subG1 phase as compared to the control (0 ppm). Bar graphs (c) depict the statistical results of 3 assays. Vertical bar indicates standard deviation of the mean. The [*] indicates P<0.05 as compared to the control (0 ppm) cells. d) Mitotracker staining assay revealed that the mitochondria (bright red) accumulate near the perinuclear region after FA treatment.</p