Effects of edaravone on cytosol cytochrome C release as measured by western blot analysis.

<p>Results indicate that edaravone treatment attenuates hyperosmotic exposure (400–450 mOsM)-induced release of cytochrome C. (A) Cytosolic fractions; (B) Mitochondrial fractions (ANOVA:*, P<0.001 vs. non-treatment control; #, P<0.001 vs. 450 mOsM treated group).</p

Effects of edaravone on Nrf2 target gene expression in primary HCEpiCs.

<p>Cells were treated with edaravone for 24 h. Gene expression was determined by real-time PCR analysis. (A) HO-1 mRNA; (B) GPx-1 mRNA; (C) GCLC mRNA; (D) GSH levels (ANOVA:*, P<0.001 vs. non-treatment control; #, P<0.001 vs. 450 mOsM treated group).</p

Edaravone prevents cell death induced by exposure to hyperosmotic media (400–450 mOsM).

<p>(A) Cell viability was measured by MTT assay; (B) Cell death was measured by LDH release assay. All experiments were repeated at least three times (ANOVA: *, P<0.001 vs. untreated control; #, P<0.001 vs. 450 mOsM treated group).</p

Effects of edaravone on cleaved caspase-3 as measured by western blot analysis.

<p>Edaravone treatment inhibits the increase in cleaved caspase-3 induced by hyperosmotic exposure (400–450 mOsM) (ANOVA:*, P<0.001 vs. non-treatment control; #, P<0.001 vs. 450 mOsM treated group).</p

Effects of edaravone on the expression of Nrf2.

<p>Levels of Nrf2 in whole cell extracts were determined by the western blot analysis (ANOVA:*, P<0.001 vs. non-treatment control; #, P<0.001 vs. 450 mOsM treated group).</p

Effects of edaravone on apoptosis in primary HCEpiCs exposed to hyperosmotic media (400–450 mOsM).

<p>Apoptotic cells were stained using a TUNEL Assay Kit. Nuclear DNA was stained with DAPI.</p

Suppressive effects of edaravone on ROS in primary HCEpiCs exposed to hyperosmotic media.

<p>ROS was evaluated via DCFH-DA staining (ANOVA: *, P<0.001 vs. untreated control; #, P<0.001 vs. 450 mOsM treated group).</p

Suppressive effects of edaravone on mitochondrial function at 10 or 20 μM.

<p>ROS in primary HCEpiCs exposed to hyperosmotic media (400–450 mOsM), evaluated via MitoSox Red staining (ANOVA: *, P<0.001 vs. untreated control; #, P<0.001 vs. 450 mOsM treated group).</p

Cu(II)-Catalyzed Synthesis of Naphthalene-1,3-diamine Derivatives from Haloalkynes and Amines

An efficient procedure for the preparation of naphthalene-1,3-diamine derivatives by copper-catalyzed haloalkynes with amines has been reported. The reaction was supposed to proceed through a coupling reaction followed by the dimerization of ynamines

Dependence of <i>Recall</i> on the diffusion macro-step.

<p>The recommendation list length is set to 20. means that we only consider the users whose degree is no larger than . Each data point is obtained by one run since the degree of a user and an item may change for different dataset divisions.</p