基本転写因子2F(TF2F)のRAP30サブユニットとRNAポリメラーゼサブユニット5(RPB5)との直接的な相互作用はTF2FとRNAポリメラーゼ2との会合に寄与する

取得学位：博士(医学)，学位授与番号：医博甲第1457号，学位授与年月日：平成13年3月22日,学位授与年：200

Metabology of Helicobacter pylori infection: role of gastrokines

Trillions of bacteria, collectively referred to as the microbiota, reside in gastrointestinal tract. Helicobacter (Campylobacter) pylori (H. pylori) is an Gram negative bacillus which infects about half of the world's population. Its causative role in gastroduodenal disease is well known. Recent studies also implicated H. pylori infection in the pathobiology of autoimmune diseases such as rheumatoid arthritis and idiopathic thrombocytopenic purpura. However, little is known about H. pylori-associated alterations in metabolic pathways and food intake as related to cardiometabolic diseases such as atherosclerosis, obesity, diabetes, and metabolic syndrome. This novel approach is conceptualized as metabology of H. pylori infection. Here we Dance Round this specific topic, with special reference to possible roles played by gastric cell-secreted molecules such as leptin, ghrelin and various cytokines, collectively designated gastrokines.Biomedical Reviews 2006; 17: 123-128

Comparative studies on the regulatory effects of raw and charred hawthorn on functional dyspepsia and intestinal flora

Purpose: To compare the effects of raw hawthorn (RH) and charred hawthorn (CH) on functional dyspepsia (FD) and intestinal flora (IF).Methods: A rat model of FD was established through use of a chronic stimulator. Rat models were evaluated by the rat’s physical state, body weight, diet, and histopathological examination. After RH or CH administration, the digestive function of the rats was evaluated by determining gastric emptying and intestinal propulsion rate, diversity of intestinal flora.Results: RH and CH both improved gastric emptying and intestinal propulsion rate in FD group when compared to control group (p < 0.05). CH yielded higher treatment effectiveness than RH. Sixteen phyla of microbiomes were recognized from all samples. After FD model establishment, the relative abundance of Lactobacillus, Lachnospiraceae and Bacteroidales decreased compared to normal control rats. On the other hand, the relative abundance of Helicobacter and Bacteroides in the model control group increased compared to normal control. After RH and CH treatment, the relative abundance of all dysregulated phyla was restored to varying degrees, but the levels after CH treatment were similar to those of the normal control group.Conclusion: The relative abundance of intestinal flora of FD model rats is significantly different from that of rats in normal control group. Thus, RH and CH intervention improves digestive function, and the mechanisms may be related to adjustment of gut dysbacteriosis.Keywords: Raw Hawthorn, Charred Hawthorn, Functional dyspepsia, Intestinal flor

G. lucidum triterpenes restores intestinal flora balance in non-hepatitis B virus-related hepatocellular carcinoma: evidence of 16S rRNA sequencing and network pharmacology analysis

Background: Ganoderma lucidum (G. lucidum) is a popular traditional remedy medicine used in Asia to promote health and longevity, which has also been highlighted for anti-cancer effects. This study investigated the molecular pharmacological mechanism of G. lucidum triterpenes in influencing intestinal flora imbalance in non-hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) based on 16S rRNA sequencing technology and network pharmacology analysis.Methods: 16S rRNA sequencing data of fecal samples from normal controls and HCC patients were obtained from the SRA database. G. lucidum triterpenes and HCC-related targets were screened by BATMAN-TCM, ETCM, and GeneCards databases. The TCGA-LIHC dataset was downloaded through the TCGA database to analyze the differential expression of key genes. NHBV-related HCC-related transcriptome RNA sequencing dataset was downloaded via the GEO database.Results: Abundance of intestinal flora in the HBV-related HCC and NHBV-related samples was higher than that of control samples. The intestinal flora of NHBV samples was mainly enriched in apoptosis and p53 pathways. Totally, 465 G. lucidum triterpenes-related targets were intersected with 4186 HCC-related targets, yielding 176 intersected targets. Among them, apoptosis and p53 pathway factors were located at the core of the protein-protein interactions network. Ganosporelactone B, the active component of G. lucidum triterpenes, had the lowest binding free energy to CASP3. CASP3 expression were upregulated in HCC tissue samples, and had higher predictive value in NHBV-related HCC patients.Conclusion: Therefore, Ganosporelactone B, the active ingredient of G. lucidum triterpenes, improves the imbalance of intestinal flora and ultimately curtails development of NHBV-related HCC

County-Scale Spatial Distribution of Soil Enzyme Activities and Enzyme Activity Indices in Agricultural Land: Implications for Soil Quality Assessment

Here the spatial distribution of soil enzymatic properties in agricultural land was evaluated on a county-wide (567 km2) scale in Changwu, Shaanxi Province, China. The spatial variations in activities of five hydrolytic enzymes were examined using geostatistical methods. The relationships between soil enzyme activities and other soil properties were evaluated using both an integrated total enzyme activity index (TEI) and the geometric mean of enzyme activities (GME). At the county scale, soil invertase, phosphatase, and catalase activities were moderately spatially correlated, whereas urease and dehydrogenase activities were weakly spatially correlated. Correlation analysis showed that both TEI and GME were better correlated with selected soil physicochemical properties than single enzyme activities. Multivariate regression analysis showed that soil OM content had the strongest positive effect while soil pH had a negative effect on the two enzyme activity indices. In addition, total phosphorous content had a positive effect on TEI and GME in orchard soils, whereas alkali-hydrolyzable nitrogen and available potassium contents, respectively, had negative and positive effects on these two enzyme indices in cropland soils. The results indicate that land use changes strongly affect soil enzyme activities in agricultural land, where TEI provides a sensitive biological indicator for soil quality