Co-expression of Gbeta 5 Enhances the Function of Two Ggamma Subunit-like Domain-containing Regulators of G Protein Signaling Proteins

Regulators of G protein signaling (RGS) stimulate the GTPase activity of G protein Galpha subunits and probably play additional roles. Some RGS proteins contain a Ggamma subunit-like (GGL) domain, which mediates a specific interaction with Gbeta 5. The role of such interactions in RGS function is unclear. RGS proteins can accelerate the kinetics of coupling of G protein-coupled receptors to G-protein-gated inwardly rectifying K+ (GIRK) channels. Therefore, we coupled m2-muscarinic acetylcholine receptors to GIRK channels in Xenopus oocytes to evaluate the effect of Gbeta 5 on RGS function. Co-expression of either RGS7 or RGS9 modestly accelerated GIRK channel kinetics. When Gbeta 5 was co-expressed with either RGS7 or RGS9, the acceleration of GIRK channel kinetics was strongly increased over that produced by RGS7 or RGS9 alone. RGS function was not enhanced by co-expression of Gbeta 1, and co-expression of Gbeta 5 alone had no effect on GIRK channel kinetics. Gbeta 5 did not modulate the function either of RGS4, an RGS protein that lacks a GGL domain, or of a functional RGS7 construct in which the GGL domain was omitted. Enhancement of RGS7 function by Gbeta 5 was not a consequence of an increase in the amount of plasma membrane or cytosolic RGS7 protein

Distance Dependent Offsets between Parallaxes for Nearby Stars and Gaia DR1 Parallaxes

We use 612 single stars with previously published trigonometric parallaxes placing them within 25 pc to evaluate parallaxes released in Gaia's first data release (DR1). We find that the Gaia parallaxes are, on average, $0.24 \pm 0.02$ mas smaller than the weighted mean trigonometric parallax values for these stars in the solar neighborhood. We also find that the offset changes with distance out to 100 pc, in the sense that the closer the star, the larger the offset. We find no systematic trends in the parallax offsets with stellar $V$ magnitude, $V-K$ color, or proper motion. We do find that the offset is roughly twice as large for stars south of the ecliptic compared to those that are north.Comment: 14 pages, 2 figures, 3 tables, accepted for publication in ApJ Letter. The table 1 is available in its entirety in a machine-readable form in the online journa

The Solar Neighborhood. XXXIV. A Search for Planets Orbiting Nearby M Dwarfs using Astrometry

Astrometric measurements are presented for seven nearby stars with previously detected planets: six M dwarfs (GJ 317, GJ 667C, GJ 581, GJ 849, GJ 876, and GJ 1214) and one K dwarf (BD $-$10 3166). Measurements are also presented for six additional nearby M dwarfs without known planets, but which are more favorable to astrometric detections of low mass companions, as well as three binary systems for which we provide astrometric orbit solutions. Observations have baselines of three to thirteen years, and were made as part of the RECONS long-term astrometry and photometry program at the CTIO/SMARTS 0.9m telescope. We provide trigonometric parallaxes and proper motions for all 16 systems, and perform an extensive analysis of the astrometric residuals to determine the minimum detectable companion mass for the 12 M dwarfs not having close stellar secondaries. For the six M dwarfs with known planets, we are not sensitive to planets, but can rule out the presence of all but the least massive brown dwarfs at periods of 2 - 12 years. For the six more astrometrically favorable M dwarfs, we conclude that none have brown dwarf companions, and are sensitive to companions with masses as low as 1 $M_{Jup}$ for periods longer than two years. In particular, we conclude that Proxima Centauri has no Jovian companions at orbital periods of 2 - 12 years. These results complement previously published M dwarf planet occurrence rates by providing astrometrically determined upper mass limits on potential super-Jupiter companions at orbits of two years and longer. As part of a continuing survey, these results are consistent with the paucity of super-Jupiter and brown dwarf companions we find among the over 250 red dwarfs within 25 pc observed longer than five years in our astrometric program.Comment: 18 pages, 5 figures, 4 tables, accepted for publication in A

The Solar Neighborhood XXV: Discovery of New Proper Motion Stars with 0.40 "/yr > mu > 0.18 "/yr between Declinations -47 degrees and 00 degrees

We present 2817 new southern proper motion systems with 0.40 "/yr > mu > 0.18 "/yr and declination between -47 degrees and 00 degrees. This is a continuation of the SuperCOSMOS-RECONS (SCR) proper motion searches of the southern sky. We use the same photometric relations as previous searches to provide distance estimates based on the assumption that the objects are single main sequence stars. We find 79 new red dwarf systems predicted to be within 25 pc, including a few new components of previously known systems. Two systems - SCR 1731-2452 at 9.5 pc and SCR 1746-3214 at 9.9 pc - are anticipated to be within 10 pc. We also find 23 new white dwarf candidates with distance estimates of 15-66 pc, as well as 360 new red subdwarf candidates. With this search, we complete the SCR sweep of the southern sky for stars with mu > 0.18 "/yr and R_59F < 16.5, resulting in a total of 5042 objects in 4724 previously unreported proper motion systems. Here we provide selected comprehensive lists from our SCR proper motion search to date, including 152 red dwarf systems estimated to be within 25 pc (nine within 10 pc), 46 white dwarfs (ten within 25 pc), and 598 subdwarf candidates. The results of this search suggest that there are more nearby systems to be found at fainter magnitudes and lower proper motion limits than those probed so far.Comment: 47 pages, 16 of text. 7 figure