Effect of Growth Stage on the Relationship Between Tan Spot and Spot Blotch Severity and Yield in Winter Wheat

Foliar fungal diseases frequently cause significant economic losses in the hard red winter wheat production areas of the Great Plains of the United States. In 2007, field experiments were conducted in four environments in Nebraska, USA to determine the crop growth stage at which severity of tan spot and spot blotch was most strongly related to yield in winter wheat. Secondary objectives were to evaluate the efficacy of fungicides in controlling tan spot and spot blotch and to determine the effect of fun¬gicide application timing on disease intensity and yield. Disease severity assessed at Zadoks growth stage (ZGS) 60 (flower¬ing) had the strongest relationship to yield at all four locations (0.72 ≤ R2 ≤ 0.90, P \u3c 0.0001). Disease severity assessed at ZGS 71 (kernel watery ripe) also was strongly related to yield (0.54 ≤ R2 ≤ 0.87, P ≤ 0.0011), but not as consistently across the four loca¬tions as disease severity assessed at ZGS 60. The relationship between yield and area under the disease progress curve (AUDPC) (0.43 ≤ R2 ≤ 0.80, P ≤ 0.0055) was weaker and less consistent across the four locations than the relationship between yield and dis¬ease severity assessed at ZGS 60 or ZGS 71. Disease progress was faster at Mead (southeast) and Clay Center (south central) than at North Platte (west central) and Sidney (west). The fungicides azoxystrobin, pyraclostrobin, propiconazole, azoxystrobin plus propiconazole, and trifloxystrobin plus propiconazole effectively reduced disease severity and AUDPC. Out of a total of 60 fun¬gicide treatments at four locations, 98%, 100%, and 100% significantly (P = 0.05) reduced disease severity, reduced AUDPC, and increased yield, respectively, compared to the check. Yield losses ranging from 27% to 42% were prevented by fungicide applica¬tions. There was no consistent effect on disease intensity or on yield of timing fungicide applications at ZGS 31 (first node on the stem detectable) versus ZGS 39 (ligule/collar of flag leaf just visible). The results from this study suggest that (i) the best predic¬tor of yield loss caused by tan spot and spot blotch in winter wheat in Nebraska is disease severity assessed at flowering and (ii) fungicides can prevent significant yield losses from tan spot and spot blotch in winter wheat

Wheat streak mosaic virus: a century old virus with rising importance worldwide

Wheat streak mosaic virus (WSMV) causes wheat streak mosaic, a disease of cereals and grasses that threatens wheat production worldwide. It is a monopartite, positive-sense, single-stranded RNA virus and the type member of the genus Tritimovirus in the family Potyviridae. The only known vector is the wheat curl mite (WCM, Aceria tosichella), recently identified as a species complex of biotypes differing in virus transmission. Low rates of seed transmission have been reported. Infected plants are stunted and have a yellow mosaic of parallel discontinuous streaks on the leaves. In the autumn, WCMs move from WSMV-infected volunteer wheat and other grass hosts to newly emerged wheat and transmit the virus which survives the winter within the plant, and the mites survive as eggs, larvae, nymphs or adults in the crown and leaf sheaths. In the spring/summer, the mites move from the maturing wheat crop to volunteer wheat and other grass hosts and transmit WSMV, and onto newly emerged wheat in the fall to which they transmit the virus, completing the disease cycle. WSMV detection is by enzyme-linked immunosorbent assay (ELISA), reverse transcription-polymerase chain reaction (RT-PCR) or quantitative RT-PCR (RT-qPCR). Three types of WSMV are recognized: A (Mexico), B (Europe, Russia, Asia) and D (USA, Argentina, Brazil, Australia, Turkey, Canada). Resistance genes Wsm1, Wsm2 and Wsm3 have been identified. The most effective, Wsm2, has been introduced into several wheat cultivars. Mitigation of losses caused by WSMV will require enhanced knowledge of the biology of WCM biotypes and WSMV, new or improved virus detection techniques, the development of resistance through traditional and molecular breeding, and the adaptation of cultural management tactics to account for climate change

Impact of \u3ci\u3eWheat streak mosaic virus\u3c/i\u3e and \u3ci\u3eTriticum mosaic virus\u3c/i\u3e Coinfection of Wheat on Transmission Rates by Wheat Curl Mites

Wheat streak mosaic virus (WSMV) and Triticum mosaic virus (TriMV) are transmitted by the wheat curl mite (WCM, Aceria tosichella), and coinfections of wheat by these viruses are common in the field. Previous work has shown that mite genotypes vary in their ability to transmit TriMV. However, the degree to which coinfection of wheat modifies WCM vector competence has not been studied. The objective was to determine whether mite genotypes differed in virus transmission ability when feeding on wheat coinfected by WSMV and TriMV. First, WCM genotype type 2 was used to determine virus transmission rates from mock-, WSMV-, TriMV-, and coinfected wheat plants. Transmission rates were determined by using single-mite transfers from replicated source plants. Coinfection reduced WSMV transmission by type 2 WCM from 50 to 35.6%; however, coinfection increased TriMV transmission from 43.3 to 56.8%. Mite survival on single-mite transfer test plants indicates that the reduction in WSMV transmission may result from poor mite survival when TriMV is present. In a second study, two separate colonies of WCM genotype type 1 were tested to assess the impact of coinfection on transmission. Type 1 mites did not transmit TriMV from coinfected plants but the two colonies varied in transmission rates for WSMV (20.9 to 36.5%). Even though these changes in mite transmission rates are moderate, they help explain the high relative incidence of TriMV-positive plants that are coinfected with WSMV in field observations. These findings begin to demonstrate the complicated interactions found in this mite–virus complex

Foliar Fungicides in Seed Corn Production

Since 1982, we have conducted a program to determine the fungicides that may be effective for controlling foliar diseases in inbred and hybrid com. For the past seven years we have limited our research to only inbreds or sister line hybrids. In 1990, we started a cooperative program with seed companies and have conducted our research in commercial seed production fields in five greatly different years in terms of weather patterns. Thirty experiments have been established in seed production fields and 25 fields have been harvested for yield. Five experiments were abandoned because of herbicide injury interactions (2,4-D) with the fungicides, excessive Stewarts disease (a bacterial disease that can not be controlled with fungicides and was devastating), or drought

Effects of fungicide application timing and cultivar resistance on Fusarium head blight and deoxynivalenol in winter wheat

Fusarium graminearum causes Fusarium head blight (FHB) in wheat. FHB reduces yield and quality and contaminates grain with the mycotoxin deoxynivalenol (DON). Effective management strategies are needed. The objectives of this research were to 1) Determine the effect of fungicide application timing at anthesis (the standard timing) and 6 and 12 days later on FHB and DON in the winter wheat cultivars Overley (susceptible) and Overland (moderately resistant) and 2) Compare the effects of a triazole and a strobilurin fungicide on FHB and DON in Overley and Overland. In 2015 two field trials (irrigated and rain-fed) were conducted in Nebraska, USA. The triazole Prosaro (prothioconazole + tebuconazole) and the strobilurin Headline (pyraclostrobin) were applied with a CO2-powered backpack sprayer at anthesis and 6 and 12 days later. A split plot design in randomized complete blocks with 4 replications was used. Main plots were cultivars and subplots were fungicide treatments. FHB index and DON were significantly (P \u3c 0.05) lower in Overland than in Overley. The window of fungicide application to control FHB and DON was widened from anthesis to 6 days later without loss of efficacy. Headline was less effective than Prosaro in controlling FHB and DON. Moderate resistance combined with a triazole fungicide most effectively reduced FHB and DON. The results indicate a wider fungicide application window and the effectiveness of combining resistance with a triazole fungicide

Impact of Timing and Method of Virus Inoculation on the Severity of Wheat Streak Mosaic Disease

Wheat streak mosaic virus (WSMV), transmitted by the wheat curl mite Aceria tosichella, frequently causes significant yield loss in winter wheat throughout the Great Plains of the United States. A field study was conducted in the 2013–14 and 2014–15 growing seasons to compare the impact of timing of WSMV inoculation (early fall, late fall, or early spring) and method of inoculation (mite or mechanical) on susceptibility of winter wheat cultivars Mace (resistant) and Overland (susceptible). Relative chlorophyll content, WSMV incidence, and yield components were determined. The greatest WSMV infection occurred for Overland, with the early fall inoculations resulting in the highest WSMV infection rate (up to 97%) and the greatest yield reductions relative to the control (up to 94%). In contrast, inoculation of Mace resulted in low WSMV incidence (1 to 28.3%). The findings from this study indicate that both method of inoculation and wheat cultivar influenced severity of wheat streak mosaic; however, timing of inoculation also had a dramatic influence on disease. In addition, mite inoculation provided much more consistent infection rates and is considered a more realistic method of inoculation to measure disease impact on wheat cultivars

What’s New in Plant Pathology

Disease Management Products During the past year several new products have become available for disease management. The new products are summarized in Tables 1 and 2, as well as included in the 2014 Guide for Weed Management in Nebraska with Insecticide and Fungicide Information. In addition, fungicides labeled for use on sorghum and sunflower have also been added to the publication. Table 1. New Foliar Fungicides Table 2. New Seed Nematicide Disease Identification and Management Resource

What\u27s New in Plant Pathology: Resistance: Mystery and Misunderstandings

One of the most common management recommendations for plant diseases is the use of resistant or tolerant varieties/hybrids in your production system. However, there is common confusion on the definition and differentiation of susceptible, tolerant, and resistant varieties/hybrids from a plant pathology viewpoint. A susceptible variety/hybrid allows the pathogen to reproduce and causes significant disease development and in turn compromises the productivity of the plant (i.e., yield). A tolerant variety/hybrid allows the pathogen to reproduce and cause disease at the same or at a slightly reduced rate as a susceptible variety/cultivar; however, there is no noticeable reduction in the plant’s overall productivity. Finally, a resistant variety/hybrid limits or prevents pathogen reproduction and disease development; hence, plant productivity is little or not affected while the plant remains very productive. It is important to note that plant resistance is not plant “immunity,” where it is expected that a variety/hybrid will have NO disease. Unfortunately, immunity does not exist for the majority of plant diseases and expecting such a reaction (or lack thereof) is unrealistic. Resistance, simply, is a reduction in disease severity due to the plant’s defenses. Plants have many mechanisms for defense but do not possess immune systems comparable to our own that preclude infection and disease development

Major Fusarium Diseases on Corn, Wheat, and Soybeans in Nebraska

Fusarium species have been associated with many important diseases of corn, wheat, and soybean, causing significant yield loss in Nebraska; some produce mycotoxins that are harmful to both human and animal consumers. These pathogens are very common in agricultural field soil across the Midwest and cause numerous types of diseases