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Not AvailableThere has been an increasing demand for alien fish species in India. However,environmental, socioeconomic and biodiversity issues are important consider-ations when regulating the unauthorized culture and spread of alien species.Information collected in the present review revealed the presence of over 300alien species imported intentionally or illegally; 291 ornamental species, 31aquaculture species and two larvicidal fishes. Field data demonstrated the wide-spread occurrence of some of the world’s worst invasive species, for example,Cyprinus carpio, Oreochromis niloticus, Aristichthys nobilis, Pygocentrus nattereriand Pterygoplichthys spp., in inland waters; other species are emerging as athreat to aquatic biodiversity. The benefits and risks of alien species were analy-sed and the impact was quantified using a simple developed ‘Fish InvasivenessScreening Test’ (FIST). Our information suggests that alien species, despitepossessing some attractive culture characteristics, generally reduce the availabil-ity of local species and establish in natural water bodies becoming invasive andconsequently adversely affecting fish biodiversity and aquatic ecosystems. Inlight of the adverse ecological impacts of alien species, we developed strategicregulations and quarantine procedures and advised aquaculturists to strictlyfollow them to contain potential and plausible menaces.Ministry of Agriculture, Department of Animal Husbandry, Dairying and Fisherie

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Not AvailableThe partial sequences of 16S rRNA and cytochrome c oxidase subunit I (COI) mitochondrial genes were analyzed for species identification and phylogenetic relationships among the commercially important Indian sciaenids (Otolithes cuvieri, Otolithes ruber, Johnius dussumieri, Johnius elongatus, Johnieops vogleri, Otolithoides biauritus and Protonibea diacanthus). Sequence analysis of both genes revealed that the seven species fell into three distinct groups, which were genetically distant from each other and exhibited identical phylogenetic resolution. Partial sequences of both the genes provided sufficient phylogenetic information to distinguish the seven sciaenids indicating the usefulness of mtDNA-based approach in species identification.Not Availabl

<span style="font-size:15.0pt;line-height:115%;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman"; mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language:MR">Classical taxonomy and 16S rRNA gene marker confirmed first record of the Menippid crab <i style="mso-bidi-font-style:normal">Menippe rumphii</i> (Fabricius, 1798) from the West coast of India</span>

76-82The Menippe rumphii (Fabricius, 1798),  marine crab,  occurring along the rocky shores of coastal areas of east coast of India was located along  the rocky shores of the Konkan coastal region of Maharashtra.  Classical taxonomy is applied for identification of this crab.  Taxonomic descriptions and morphometric analysis confirmed the species as Menippe rumphii. The 16S rRNA gene from Menippe rumphii adults was sequenced to corroborate species identification. Phylogenetic analysis showed ostensible clade between sequences of Menippe rumphii from NCBI and study crab.  Classical taxonomy and 16S rRNA gene marker substantiated the record of the specimens from the Konkan coast. </span

Development and characterization of two new cell lines from common carp, Cyprinus carpio (Linn)

Two new cell lines (CCF and CCH) were established from fin and heart tissues of common carp, Cyprinus carpio. The cells were optimally maintained in Leibovitz-15 medium supplemented with 10% fetal bovine serum (FBS) and 10 ng/ml of basic fibroblastic growth factor (bFGF). The effects of temperature, concentration of FBS and bFGF on the growth of CCF and CCH cells were examined. The temperature ranged from 24 to 32 °C for good growth of the cells. The growth rate of cells was higher in medium containing 10% FBS and the addition of bFGF to the medium significantly increased the growth rate. The CCF cells were found to be epithelial, while the CCH cells were fibroblastic in nature. The cytogenetic analysis of the cell lines revealed a diploid number of 100 chromosomes in C. carpio. The viability of CCF and CCH cell lines were 70 and 72%, respectively, after six months of storage in liquid nitrogen (-196 ° C). Molecular characterization of the cell lines using 16S rRNA and Cytochrome Oxidase Subunit I (COI) revealed the origin of the cell lines. These new cell lines will be useful for isolation of fish viruses and other in vitro biotechnological studies

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Not AvailableA new cell culture system (MRH) was developed for the first time from 2 months old freshwater prawn, Macrobrachium rosenbergii. Primary cultures were developed from heart tissues by explant culture technique. Cell outgrowth was obtained from the heart explant after 14 days of explant culture. The culture medium used was Leibovitz-15 supplemented with 20% Fetal Bovine Serum along with 1% prawn hemolymph serum, 0.1% glucose, 0.5% NaCl and antibiotics (Penicillin 10,000 Units ml−1, Streptomycin 10,000 μg ml−1, Amphotericin B 500 mg ml−1) with a final osmomolality of 470–550 mmol kg−1. The pH of the growth medium found suitable for the growth of the cells was 7.20. The viability of cells was found to be 60% when revived after a month of storage in liquid nitrogen.CSIR, New Delh

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Not AvailableTwo new cell lines (CCF and CCH) were established from fin and heart tissues of common carp, Cyprinus carpio. The cells were optimally maintained in Leibovitz-15 medium supplemented with 10% fetal bovine serum (FBS) and 10 ng/ml of basic fibroblastic growth factor (bFGF). The effects of temperature, concentration of FBS and bFGF on the growth of CCF and CCH cells were examined. The temperature ranged from 24 to 32 °C for good growth of the cells. The growth rate of cells was higher in medium containing 10% FBS and the addition of bFGF to the medium significantly increased the growth rate. The CCF cells were found to be epithelial, while the CCH cells were fibroblastic in nature. The cytogenetic analysis of the cell lines revealed a diploid number of 100 chromosomes in C. carpio. The viability of CCF and CCH cell lines were 70 and 72%, respectively, after six months of storage in liquid nitrogen (-196 ° C). Molecular characterization of the cell lines using 16S rRNA and Cytochrome Oxidase Subunit I (COI) revealed the origin of the cell lines. These new cell lines will be useful for isolation of fish viruses and other in vitro biotechnological studies.Not Availabl

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Not AvailableGenetic variability was assessed among three species of genus Channa, namely C. gachua, C. punctata and C. striata, using karyological tools including karyomorphology, silver nitrate and chromomycin A3 staining of nucleolar organizer regions (NORs) and C-banding. Additionally, sequence variation in partial 18S and divergent domain 1 of 28S ribosomal DNA was utilized for inter-species diversity assessment. Diploid chromosome numbers of 32, 40 and 52 in the three species were observed and the karyotype formula was derived as 18m + 12sm + 2st (fundamental arm number [FN] = 62); 6m + 2sm + 10st + 22t (FN = 48) and 12m + 10sm + 14st + 16t (FN = 86) in C. punctata, C. striata and C. gachua, respectively. Similarly, variations were also observed in number and location of silver and chromomycin A3 stained NORs and C-banding patterns. DNA sequencing generated sizes for 18S of 243, 248, 245 bp and for 28S of 401, 392 and 393 bp in C. gachua, C. punctata and C. striata, respectively. The results indicated that the cytogenetic and molecular markers are useful for the assessment of genetic diversity among the fish species and could provide valuable information for germplasm evaluation and ex situ conservation.Not Availabl

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Not AvailableThe present study describes the annual condition as well the length–weight (LWR) of the Asian striped catfish, Mystus vittatus (Bloch, 1794), an important fishery by the village poor Anglers. A total of 119 specimens (7.0–8.8 cm standard length, 8.0–10.9 cm total length) used in this study was caught with traditional fishing gear from July 2009 to August 2009. Overall, the allometric coefficient ‘b’ of the LWR was close to the isometric value (b = 2.88). The results further indicated that the LWRs were highly correlated (r2 = 0.826296, P < 0.01). The aim of the present paper was to carry out the comprehensive description of the LWRs and condition of the M. vittatus. In conclusion, this study has provided basic information on LWR, and condition. These results will be useful for fishery biologists and managers to impose adequate regulations for sustainable fishery management in the non-drainable ponds in India.Not Availabl

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Not AvailableThe randomly amplified polymorphic DNA (RAPD) markers were used to detect interspecific genetic variability and genetic relatedness among five Indian sciaenids namely Otolithes cuvieri, Johnieops sina, Johnieops macrorhynus, Johnieops vogleri and Protonibea diacanthus for the first time. Eight RAPD primers (OPA01, OPA06, OPA07, OPA18, OPP12, OPP14, OPP16 and OPP11) generated 40 species specific diagnostic bands. The highest genetic divergence was detected between J. macrorhynus and P. diacanthus (0.586) where as the lowest one was observed between J. sina and J. vogleri(0.274)Not Availabl