TOLERANCE TO THE IMPAIRING EFFECTS OF ALCOHOL ON THE INHIBITION AND ACTIVATION OF BEHAVIOR

Moderate doses of alcohol impair response inhibition activation. Recent work has shown that, during a single dose, response inhibition recovers from the impairing effects of alcohol more slowly than response activation. Evidence for a lag in tolerance development to inhibitory versus activational mechanisms suggests that, as blood alcohol declines, drinkers’ response inhibition might continue to be impaired, despite the recovery of response activation. However, this has not been studied across repeated doses. This study examined how cross-session tolerance to alcohol develops differentially between response activation and inhibition. Thirty-two healthy adults performed a cued go/no-go task that measured response activation and inhibition. The study tested the degree to which response activation and inhibition developed acute and cross-session tolerance to a moderate dose of alcohol (0.65 g/kg) administered twice. Alcohol impaired response activation and inhibition during both administrations. Response activation displayed acute tolerance to alcohol during both administrations and cross-session tolerance from the first to second administration. Response inhibition was impaired by each alcohol administration but showed no acute or cross-session tolerance. Evidence of biased recovery of response activation over inhibition during a single dose and as doses are repeated could contribute to some of the impulsive behavior commonly observed under alcohol

Distributed Cinema: Interactive, Networked Spectatorship In The Age Of Digital Media

Digital media has changed much of how people watch, consume and interact with digital media. The loss of indexicality, or the potential infidelity between an image and its source, contributes to a distrust of images. The ubiquity of interactive media changes aesthetics of images, as viewers begin to expect interactivity. Networked media changes not only the ways in which viewers access media, but also how they communicate with each other about this media. The Tulse Luper Suitcases encapsulates all of these phenomena

Gene activation using FLP recombinase in C. elegans

Journal ArticleThe FLP enzyme catalyzes recombination between specific target sequences in DNA. Here we use FLP to temporally and spatially control gene expression in the nematode C. elegans. Transcription is blocked by the presence of an "off cassette" between the promoter and the coding region of the desired product. The "off cassette" is composed of a transcriptional terminator flanked by FLP recognition targets (FRT). This sequence can be excised by FLP recombinase to bring together the promoter and the coding region. We have introduced two fluorescent reporters into the system: a red reporter for promoter activity prior to FLP expression and a green reporter for expression of the gene of interest after FLP expression. The constructs are designed using the multisite Gateway system, so that promoters and coding regions can be quickly mixed and matched

Virtually There: Social Structure Over Time and Space

Communication is both process and art. It is the vehicle through which we share our thoughts and feelings as much as it is a creative activity resulting in the production of new meanings and ways of knowing. While we commonly understand communication as a continual process of reception and interpretation, new electronic media continue to extend our knowledge of what constitutes communication and how we characterize communication. Challenging the longstanding assumptions of fixed and positioned ways of knowing, studies with new electronic media accentuate the burgeoning contrast between the authority and stability of the written word and the mutability of the electronic word (Craig & Muller, 2007). These studies also increasingly highlight the need to problematize virtual communication in its own terms, examining it as a “technologizing” of message dissemination and reception and construction of the self over time and space (Gozzi & Haynes, 1992; Ong, 2002)

Assessment of targeting accuracy of a low-energy stereotactic radiosurgery treatment for age-related macular degeneration

Age-related macular degeneration (AMD), a leading cause of blindness in the United States, is a neovascular disease that may be controlled with radiation therapy. Early patient outcomes of external beam radiotherapy, however, have been mixed. Recently, a novel multimodality treatment was developed, comprising external beam radiotherapy and concomitant treatment with a vascular endothelial growth factor inhibitor. The radiotherapy arm is performed by stereotactic radiosurgery, delivering a 16 Gy dose in the macula (clinical target volume, CTV) using three external low-energy x-ray fields while adequately sparing normal tissues. The purpose of our study was to test the sensitivity of the delivery of the prescribed dose in the CTV using this technique and of the adequate sparing of normal tissues to all plausible variations in the position and gaze angle of the eye. Using Monte Carlo simulations of a 16 Gy treatment, we varied the gaze angle by ±5° in the polar and azimuthal directions, the linear displacement of the eye ±1mm in all orthogonal directions, and observed the union of the three fields on the posterior wall of spheres concentric with the eye that had diameters between 20 and 28 mm. In all cases, the dose in the CTV fluctuated \u3c6%, the maximum dose in the sclera was \u3c20 Gy, the dose in the optic disc, optic nerve, lens and cornea were \u3c0.7 Gy and the three-field junction was adequately preserved. The results of this study provide strong evidence that for plausible variations in the position of the eye during treatment, either by the setup error or intrafractionmotion, the prescribed dose will be delivered to the CTV and the dose in structures at risk will be kept far below tolerance doses. © 2010 Institute of Physics and Engineering in Medicine

Rapid single nucleotide polymorphism mapping in C. elegans

BACKGROUND: In C. elegans, single nucleotide polymorphisms (SNPs) can function as silent genetic markers, with applications ranging from classical two- and three-factor mapping to measuring recombination across whole chromosomes. RESULTS: Here, we describe a set of 48 primer pairs that flank SNPs evenly spaced across the C. elegans genome and that work under identical PCR conditions. Each SNP in this set alters a DraI site, enabling rapid and parallel scoring. We describe a procedure using these reagents to quickly and reliably map mutations. We show that these techniques correctly map a known gene, dpy-5. We then use these techniques to map mutations in an uncharacterized strain, and show that its behavioral phenotype can be simultaneously mapped to three loci. CONCLUSION: Together, the reagents and methods described represent a significant advance in the accurate, rapid and inexpensive mapping of genes in C. elegans