An Snp Marker Potentially Linked to Somatic Embryogenesis of Oil Palm (Elaeis Guineensis)

Oil palm (Elaeis guineensis) is one of the most important oil-bearing crop in the world. This crop can be vegetatively propagated only using tissue culture technique. Oil palm tissue culture technique has low efficiency, with callogenesis and embryogenesis stages as the limiting factors. Genetic factor has a major role in determining the success rate of these two stages. The use of molecular markers which represent the rate of embryogenesis or callogenesis has the potential to improve the efficiency of oil palm tissue culture process. In this study, SNP mining was conducted on embryogenesis transcriptome data, oil palm cDNA database, oil palm genome database, and oil palm SNPs marker database in NCBI. The objective of this study was to obtain SNP marker which represents the embryogenesis potential, to be further used in marker assisted selection of oil palm ortets. One SNP (EMB6) showed significant association with embryogenesis rate. This SNP was found in one of Auxin Response Factor (ARF) family gene. Nucleotide replacement from Adenine to Guanine changed the 307th amino acid from Isoleucine to Methionine. Oil palms with Adenine homozygote (A/A) pattern on the EMB6 showed 8-fold higher chance to produce significantly higher embryogenesis rate than Adenine-Guanine heterozygote (A/G)

Purification of methanol dehydrogenase from mouth methylotrophic bacteria of tropical region

Aims: Purification of methanol dehydrogenase (MDH) from methylotrophic bacteria was conducted to obtain pure enzyme for further research and industrial applications due to the enzyme’s unique activity that catalyzes oxidation of methanol as an important carbon source in methylotrophic bacteria.Methodology and Results: The enzyme was screened from methylotrophic bacteria isolated from human mouth. Purification of this enzyme was conducted using ammonium sulphate precipitation followed by cation exchange chromatography. Two types of media were used to produce the enzymes: luria broth and standard mineral salts media (MSM). MSM produced MDH with higher specific activity than LB. Specific activity was also increased along with the purification steps. Application of ammonium sulphate increased the purity of enzyme and was more effective for the enzyme produced in LB. Using sepharose increased the enzyme activity 10 -57 folds.Conclusion, significant and impact of this study: With this, ammonium sulphate precipitation coupled with single cation exchange chromatographic system has been proved to provide sufficient purified of methanol dehydrogenase from methylotrophic bacteria origin of human mouth with high specific activity for further application