The Comprehensive Handling of Safety in an Autonomous Robot Capstone Project

A systematic approach to safety issues is described in the context of an autonomous robot capstone project. The treatment of safety should not be an ad hoc or an after-thought aspect of design projects. Engineering students need to consider safety as an integral component of the design process and to identify and address hazards systematically in each stage of project work. Appropriate actions include researching professional standards and regulations, incorporating safety best practices, developing safety checklists and operating protocols, and providing significant safety documentation. Formal safety components were added to a capstone design project for electrical and computer engineering undergraduates in which an R2D2-like robot was designed and built. The work provides project examples, lessons learned, and student feedback related to the safety treatment

Automated tagging of environmental data using a novel SKOS formatted environmental thesaurus

There is increasing need to use the widest range of data to address issues of environmental management and change, which is reflected in increasing emphasis from government funding agencies for better management and access to environmental data. Bringing together different environmental datasets to confidently enable integrated analysis requires reference to common standards and definitions, which are frequently lacking in environmental data, due to the broad subject area and lack of metadata. Automatic inclusion within datasets of controlled vocabulary concepts from publicly available standard vocabularies facilitates accurate annotation and promotes efficiency of metadata creation. To this end, we have developed a thesaurus capable of describing environmental chemistry datasets. We demonstrate a novel method for tagging datasets, via insertion of this thesaurus into a Laboratory Information Management System, enabling automated tagging of data, thus promoting semantic interoperability between tagged data resources. Being web available, and formatted using the Simple Knowledge Organisation System (SKOS) semantic standard, this thesaurus is capable of providing links both to and from other relevant thesauri, thus facilitating a linked data approach. Future developments will see extension of the thesaurus by the user community, in terms of both concepts included and links to externally hosted vocabularies. By employing a Linked Open Data approach, we anticipate that Web-based tools will be able to use concepts from the thesaurus to discover and link data to other information sources, including use in national assessment of the extent and condition of environmental resources

Glycation of Amino Groups in Protein

Ribonuclease A has been used as a model protein for studying the specificity of glycation of amino groups in protein under physiological conditions (phosphate buffer, pH 7.4, 37 “C). Incubation of RNase with glucose led to an enhanced rate of inactivation of the enzyme relative to the rate of modification of lysine residues, suggesting preferential modification of active site lysine residues. Sites of glycation of RNase were identified by amino acid analysis of tryptic peptides isolated by reverse-phase high pressure liquid chromatography and phenylboronate affinity chromatography. Schiff base adducts were trapped with Na- BH&N and the a-amino group of Lys-1 was identified as the primary site (80-90%) of initial Schiff base formation on RNase. In contrast, Lys-41 and Lys-7 in the active sitaec counted for about 38 and 29%, respectively, of ketoamine adducts formed via the Amadori rearrangement. Other sites reactive in ketoamine formation included Ne-Lys-1 (15%), N-Lys-1 (9%), and Lys-37 (9%w) hich are adjacent to acidic amino acids. The remaining six lysine residues in RNase, which are located on the surface of the protein, were relatively inactive in forming either the Schiff base or Amadori adduct. Both the equilibrium Schiff base concentration and the rate of the Amadori rearrangement at each site were found to be important in determining the specificity of glycation of RNase

Bone Tissue Engineering: Scalability and Optimization of Densified Collagen-Fibril Bone Graft Substitute Materials

Over 240 million people missing teeth worldwide experience lingering problems such as difficulty speaking and eating, undesirable aesthetics, and resorption of bone supporting neighboring teeth. The gold standard of treatment utilizes grafts to attach a function-restoring implant to supporting bone. Current graft materials suffer from problems including autologous donor site morbidity, long resorption time, incomplete integration with the maxillae or mandible, and structural weakness. Patient-specific, cellularized bone grafts may be a solution to these issues by accelerating and improving the quality of regenerated bone. Recently, encapsulation of mesenchymal stem cells within self-assembling type I collagen oligomer matrices has been shown to support rapid mineralization of small-scale bone constructs (cylinders with diameter and height of 6mm and 1mm, respectively) in vitro. However, this method’s volume and geometric constraints for nutrient transport and cell viability are still unknown. In this study, the effects of construct size and medium formulation on mineralization were investigated using conventional static culture methods. To create constructs, human adipose stem cells (hASCs) were embedded in oligomer matrices, allowed to polymerize, and compressed to final cell and fibril densities of 3x107 cells/mL and 50 mg/mL, respectively. Varying construct sizes (maximum diameter and thickness of 11 mm and 0.81 mm) were cultured for 1 week in growth medium or osteogenic medium with varying calcium concentrations. Alizarin red staining was used to detect calcium deposits indicative of cell-induced mineralization. Preliminary data suggests that culture in osteogenic medium supplemented with both 8 mM and 16 mM calcium may induce rapid, uniform mineralization across all sizes tested, and 16 mM calcium supplementation induces greater mineralization. However, additional validation by direct measurement of cell viability and osteogenic differentiation will be needed to better compare bone regeneration as a function of scale

Effect of Phosphate on the Kinetics and Specificity of Glycation of Protein

The glycation (nonenzymatic glycosylation) of several proteins was studied in various buffiner os rder to assess the effects of buffering ions on the kinetics and specificity of glycation of protein. Incubation of RNase with glucose in phosphate buffer resulted in inactivation of the enzyme because of preferential modification of lysine residues ino r near the activsei te. In contrast, in the cationic buffers, 3-(N-morpholino)propanesulfonic acid and 3-(N-tris(hydroxymethyl)rnethylamino)- 2-hydroxypropanesulfonica cid, the kineticso f glycation of RNase were decreased 2- to 3-fold, there was a decrease in glycation of active site versus peripheral lysines, and the enzyme was resistant to inactivation by glucose. The extent of Schiff base formation on RNase was comparable in the three buffers, suggesting that phosphate, bound in the active site of RNase, catalyzed the Amadori rearrangement at active site lysines, leading to the enhanced rate of inactivation of the enzyme. Phosphate catalysis of glycation was concentration-dependent and could be mimicked by arsenate. Phosphate also stimulated the rate of glycation of other proteins, such as lysozyme, cytochrome c, albumin, and hemoglobin. As with RNase, phosphate affected the specificity of glycation of hemoglobin, resulting in increasegdly cation of amino-terminal valine versus intrachain lysine residues. 2,3-Diphosphoglycerate exerted similar effeocnt st he glycation of hemoglobin, suggesting that inorganic and organic phosphates may play an important role in determining the kinetics and specificity of glycation of hemoglobin in the red cell. Overall, these studies establishth at buffering ions or ligands can exert significant effects on the kinetics ands pecificity of glycation of proteins

Development and preliminary testing of the psychosocial adjustment to hereditary diseases scale

Background: The presence of Lynch syndrome (LS) can bring a lifetime of uncertainty to an entire family as members adjust to living with a high lifetime cancer risk. The research base on how individuals and families adjust to genetic-linked diseases following predictive genetic testing has increased our understanding of short-term impacts but gaps continue to exist in knowledge of important factors that facilitate or impede long-term adjustment. The failure of existing scales to detect psychosocial adjustment challenges in this population has led researchers to question the adequate sensitivity of these instruments. Furthermore, we have limited insight into the role of the family in promoting adjustment. Methods: The purpose of this study was to develop and initially validate the Psychosocial Adjustment to Hereditary Diseases (PAHD) scale. This scale consists of two subscales, the Burden of Knowing (BK) and Family Connectedness (FC). Items for the two subscales were generated from a qualitative data base and tested in a sample of 243 participants from families with LS. Results: The Multitrait/Multi-Item Analysis Program-Revised (MAP-R) was used to evaluate the psychometric properties of the PAHD. The findings support the convergent and discriminant validity of the subscales. Construct validity was confirmed by factor analysis and Cronbach’s alpha supported a strong internal consistency for BK (0.83) and FC (0.84). Conclusion: Preliminary testing suggests that the PAHD is a psychometrically sound scale capable of assessing psychosocial adjustment. We conclude that the PAHD may be a valuable monitoring tool to identify individuals and families who may require therapeutic interventions

100 years of chemistry at Rhodes University

The history of Grahamstown is well documented and two books deal with the history of Rhodes University.1,2 Although the Chemistry Department was one of the founding departments, coverage in the official histories is minimal and sometimes inaccurate or misleading. The Rhodes University Centenary is an appropriate time to look back on some of the achievements of the department and some of its graduates over the past 100 years

The Motions of Clusters of Galaxies and the Dipoles of the Peculiar Velocity Field

In preceding papers of this series, TF relations for galaxies in 24 clusters with radial velocities between 1000 and 9200 km/s (SCI sample) were obtained, a Tully-Fisher (TF) template relation was constructed and mean offsets of each cluster with respect to the template obtained. Here, an estimate of the line-of-sight peculiar velocities of the clusters and their associated errors are given. It is found that cluster peculiar velocities in the Cosmic Microwave Background reference frame do not exceed 600 k/ms and that their distribution has a line-of-sight dispersion of 300 k/ms, suggesting a more quiescent cluster peculiar velocity field than previously reported. When measured in a reference frame in which the Local Group is at rest, the set of clusters at cz > 3000 km/s exhibits a dipole moment in agreement with that of the CMB, both in amplitude and apex direction. It is estimated that the bulk flow of a sphere of 6000 km/s radius in the CMB reference frame is between 140 and 320 km/s. These results are in agreement with those obtained from an independent sample of field galaxies (Giovanelli et al. 1998; see astro-ph/9807274)Comment: 9 pages, 2 tables, 7 figures, uses AAS LaTex; to appear in A

Diagnostic accuracy of 3.0-T magnetic resonance T1 and T2 mapping and T2-weighted dark-blood imaging for the infarct-related coronary artery in Non-ST-segment elevation myocardial infarction

Background: Patients with recent non–ST‐segment elevation myocardial infarction commonly have heterogeneous characteristics that may be challenging to assess clinically. Methods and Results: We prospectively studied the diagnostic accuracy of 2 novel (T1, T2 mapping) and 1 established (T2‐weighted short tau inversion recovery [T2W‐STIR]) magnetic resonance imaging methods for imaging the ischemic area at risk and myocardial salvage in 73 patients with non–ST‐segment elevation myocardial infarction (mean age 57±10 years, 78% male) at 3.0‐T magnetic resonance imaging within 6.5±3.5 days of invasive management. The infarct‐related territory was identified independently using a combination of angiographic, ECG, and clinical findings. The presence and extent of infarction was assessed with late gadolinium enhancement imaging (gadobutrol, 0.1 mmol/kg). The extent of acutely injured myocardium was independently assessed with native T1, T2, and T2W‐STIR methods. The mean infarct size was 5.9±8.0% of left ventricular mass. The infarct zone T1 and T2 times were 1323±68 and 57±5 ms, respectively. The diagnostic accuracies of T1 and T2 mapping for identification of the infarct‐related artery were similar (P=0.125), and both were superior to T2W‐STIR (P<0.001). The extent of myocardial injury (percentage of left ventricular volume) estimated with T1 (15.8±10.6%) and T2 maps (16.0±11.8%) was similar (P=0.838) and moderately well correlated (r=0.82, P<0.001). Mean extent of acute injury estimated with T2W‐STIR (7.8±11.6%) was lower than that estimated with T1 (P<0.001) or T2 maps (P<0.001). Conclusions: In patients with non–ST‐segment elevation myocardial infarction, T1 and T2 magnetic resonance imaging mapping have higher diagnostic performance than T2W‐STIR for identifying the infarct‐related artery. Compared with conventional STIR, T1 and T2 maps have superior value to inform diagnosis and revascularization planning in non–ST‐segment elevation myocardial infarction. Clinical Trial Registration: URL: http://www.clinicaltrials.gov. Unique identifier: NCT02073422