7 research outputs found
Determination of labile inorganic and organic species of Al and Cu in river waters using the diffusive gradients in thin films technique
Monitoring and flux determination of trace metals in rivers of the Seversky Donets basin (Ukraine) using DGT passive samplers
Ausência de Yersinia enterocolitica em alimentos, e reservatórios animais, em áreas do Estado de Pernambuco, Brasil
Methicillin-resistant Staphylococcus aureus in human milk
We collected and analyzed 500 samples of human milk, from five Brazilian cities (100 from each) to detect methicillin-resistant strains of Staphylococcus aureus (MRSA) producing enterotoxins. We found 57 strains of MRSA, and the mecA gene, responsible for resistance, was detected in all of them using a specific molecular probe. We examined 40 strains for the presence of four enterotoxins, after selecting a subset that included all strains from each region, except for the largest sample, from which 10 were randomly selected. Among these two presented enterotoxin B, and growth in human colostrum and trypicase soy broth. After 5 h of incubation at 37°C, population sizes were already higher than 9.4 x 105 UFC/ml and enterotoxin was released into culture medium and colostrum. Our results stress the importance of hygiene, sanitary measures, and appropriate preservation conditions to avoid the proliferation of S. aureus in human milk