A Mutant \u3cem\u3edec-1\u3c/em\u3e Transgene Induces Dominant Female Sterility in \u3cem\u3eDrosophila melanogaster\u3c/em\u3e

The Drosophila dec-1 gene produces three proproteins required for female fertility and eggshell assembly. The three proproteins are distinguished by their C termini. Fc106, the most abundant proprotein, is cleaved within the vitelline membrane to three mature derivatives in a developmentally regulated manner. To define sequences within fc106 that are critical for its function, we created wild-type and mutant versions of an fc106 cDNA transgene. The functional consequences of the mutations were assessed in dec-14, a female-sterile splicing mutant that does not produce the fc106 isoform. The fertility of dec-14 females was restored by the introduction of either a wild-type transgene or a transgene bearing a C-terminal deletion that included fc106-specific sequences. Surprisingly, the removal of internal coding sequences created an aberrant DEC-1 proprotein that induced female sterility when introduced into wild-type flies. Dominant female sterility was not associated with larger deletions that included the fc106 N terminus, suggesting that abnormal juxtaposition of N- and C-terminal sequences in the aberrant proprotein interfered with endogenous DEC-1 proteins. Changes in the fractionation behavior of the endogenous fc106 C-terminal derivative, s60, and morphological changes in the endochorion in response to expression of the aberrant proprotein support this interpretation

Double jeopardy: the potential for problems when bats interact with breathable roofing membranes in the United Kingdom

In order to reduce environmental impacts and achieve sustainability, it is important to balance the interactions between the built and natural environment. The construction industry is becoming more aware of ecological concerns and the importance that biodiversity and maintenance ecosystem services has for sustainability. Bats constitute an important component of urban biodiversity and several species in the UK are highly dependent on buildings, making them particularly vulnerable to anthropogenic and environmental changes. Many buildings suitable for use as bat roosts often require re-roofing as they age and traditional bituminous roofing felts are frequently being replaced with breathable roofing membranes (BRMs). In the UK new building regulations and modern materials may substantially reduce the viability of existing roosts, yet at thesame time building regulations require that materials be fit for purpose. Reports suggest that both bats and BRMs may experience problems when the two interact. Such information makes it important to understand how house dwelling bats and BRMs may be affected. This paper considers the possible ways in which bats and BRMs may interact, how this could affect existing bat roosts within buildings and the implications for BRM service life predictions and warranties. Keywords –Breathable Roofing Membranes, Bats in Buildings, Material Deterioration, Sustainability, Conservation, Biodiversi

Charting the practical dimensions of understaffing from a managerial perspective: the everyday shape of the UK’s sonographer shortage

Introduction: Across the last two decades, ultrasound services in many healthcare sectors have become increasingly pressurised as a consequence of upsurging demand and difficulties in recruiting viable clinicians. Indeed by 2013, the UK government's Migration Advisory Committee had listed sonography as an official ‘shortage specialty’. Comparatively little research has to date, however, explored the impacts of this situation upon the departments themselves, and the individuals working therein. The core purpose of this study is, thus, to lend qualitative depth to current understandings of the frontline situation in the UK's ultrasound units, many of which are understaffed, from the perspective of their managers. Methods: Using a thematic analysis informed by a Straussian model of Grounded Theory, N=20 extended accounts provided by ultrasound department leads in public (n=18) and private (n=2) units were explored. Results: Four global themes emerged from the analysis of which the first two (the broadly sociological matters) are described in this paper. Theme 1 addresses how a lack of staff in the broader ultrasound economy has created a troublesome migratory system in contemporary UK ultrasound. Theme 2 addresses how this economy works chiefly to the advantage of the most junior and the most senior clinicians, often leaving mid-career professionals in the borderline impossible situation of having to concurrently occupy both junior and senior roles. Conclusions: The findings ideally open up debate on some key practical contingencies of the UK’s sonographer shortage, and reflect upon literature regarding the nuanced aspects of a shifting healthcare workplace constitution

The future of ultrasound Advanced Clinical Practitioner education: Mapping the views of clinical leads in the public and private sector

Background: There can be little doubt that current models and mechanisms involved in ultrasound Advanced Clinical Practitioner education, at UK University Level 7, are in need of change to adapt to the challenges of a rapidly changing public healthcare environment, while functioning alongside newer ‘direct entry’ educational routes into ultrasound (Miller, Waring, Bolton and Sloane, 2018; Waring, Miller, Bolton and Sloane, 2018). This research, funded by Health Education England, explores the views of clinical leads in UK ultrasound departments regarding the facility of current education, the changes needed now and the changes that are likely necessary to future-proof the curricula. Methods: With institutional ethical approval, semi-structured interviews were conducted with N=10 clinical leads in public and private ultrasound units. Data were transcribed verbatim, and transcripts analysed using the thematic approach outlined by Braun and Clarke (2008). Results: Four interlinked global themes emerged. 1. Soft Skills: The present and increasing need for better and more flexible verbal/written communication skills, plus team working and management/leadership training. 2. Non-Genericism: The need to prepare sonographers for progressively more varied and/or difficult patients and working environments. 3. Specialisation in Diversification: The need to prepare sonographers for a greater range of diagnostic and interventional tasks in more specific domains of ultrasound. 4. Evolving CPD: Developing new CPD models to facilitate greater ongoing involvement. Conclusion: While the participants were broadly very content with current models/standards of Ultrasound ACP education, the findings underscore a range of concerns they held regarding its adaptation in the short-to-medium terms

A qualitative study of professional and carer perceptions of the threats to safe hospital discharge for stroke and hip fracture patients in the English National Health Service

Background: Hospital discharge is a vulnerable transitional stage in patient care. This qualitative study investigated the views of healthcare professionals and patients about the threats to safe hospital discharge with aim of identifying contributory and latent factors. The study was undertaken in two regional health and social care systems in the English National Health Service, each comprising three acute hospitals, community and primary care providers and municipal social care services. The study focused on the threats to safe discharge for hip fracture and stroke patients as exemplars of complex care transitions. Methods: A qualitative study involving narrative interviews with 213 representative stakeholders and professionals involved in discharge planning and care transition activities. Narratives were analysed in line with ‘systems’ thinking to identify proximal (active) and distal (latent) factors, and the relationships between them. Results: Three linked categories of commonly and consistently identified threat to safe discharge were identified:(1) ‘direct’ patient harms comprising falls, infection, sores and ulceration, medicines-related issues, and relapse; (2) proximal ‘contributing’ factors including completion of tests, assessment of patient, management of equipment and medicines, care plan, follow-up care and patient education; and distal ‘latent’ factors including discharge planning, referral processes, discharge timing, resources constraints, and organisational demands. Conclusion: From the perspective of stakeholders, the study elaborates the relationship between patient harms and systemic factors in the context of hospital discharge. It supports the importance of communication and collaboration across occupational and organisational boundaries, but also the challenges to supporting such communication with the inherent complexity of the care system

Adaptive sampling method to monitor low-risk pathways with limited surveillance resources

The rise of globalisation has led to a sharp increase in international trade, with high volumes of containers, goods and items moving across the world. Unfortunately, these trade pathways also facilitate the movement of unwanted pests, weeds, diseases, and pathogens. Each item could contain biosecurity risk material, but it is impractical to inspect every item. Instead, inspection efforts typically focus on high risk items. However, low risk does not imply no risk. It is crucial to monitor the low risk pathways to ensure that they are and remain low risk. To do so, many approaches would seek to estimate the risk to some precision, but the lower the risk, the more samples needed to estimate the risk. On a low-risk pathway that can be afforded more limited inspection resources, it makes more sense to assign fewer samples to the lower risk activities. We approach the problem by introducing two thresholds. Our method focuses on letting us know whether the risk is below certain thresholds, rather than estimating the risk precisely. This method also allows us to detect a significant change in risk. Our approach typically requires less sampling than previous methods, while still providing evidence to regulators to help them efficiently and effectively allocate inspection effort.Comment: 12 + 2 pages, 8 figures, 2 table

Soil carbon pools are affected by species identity and productivity in a tree common garden experiment

The formation and turnover of soil organic carbon (C), the largest terrestrial C pool, is strongly impacted by the ultimate source of that C: leaves, wood, roots, and root exudates. The quantity and quality of these inputs is determined by the identity of the plants involved. Yet substantial uncertainty surrounds the complex relationships among plant traits and soil C, precluding efforts to maximize whole-ecosystem C uptake in nature-based climate mitigation scenarios. In this study, we leveraged a biodiversity-ecosystem function experiment with trees (IDENT) to explore the effects of interspecific variation in plant traits on soil C dynamics in the very early stages of stand development (9 years since planting). Mineral soil C stocks to 5 cm depth were quantified in monospecific plots of 19 tree species planted on a former agricultural field, and analyzed in relation to tree growth and functional traits. We found that tree species identity affected soil bulk density and, to a lesser extent, the carbon content of the topsoil, and thereby total C pools. Among species and across plots, mineral soil C stocks were positively correlated with rates of tree growth and were significantly larger beneath broadleaf trees with “fast” functional traits vs. conifers with more conservative leaf traits, when comparisons were made over equivalent soil depth increments. Thus, plant functional traits mediate interspecific differences in productivity, which in turn influence the magnitude of belowground C stocks. These results highlight important linkages between above- and belowground carbon cycles in the earliest stages of afforestation