Respon Pertumbuhan Vegetatif Tanaman Buncis (Phaseolus vulgaris L.) Dengan Pemberian Kompos Limbah Kulit Pisang Nipah

Bean (Phaseolus vulgaris L.) is a type of legumes from leguminoceae familly which is beneficial to decrease blood pressure and control body metabolism system. Bean can grow well on rich soil which contains much humus. This research aims at identifying the effect of Nipah banana’s skin waste towards the vegetative growth of bean. The research was conducted for thee months since Febuary to April 2017. It was carried out in a lab of biology deapartment, Faculty of Mathematics and Natural Sciences, Tanjungpura Universty. The research employed completely randomized design ( RAL). It consisted of six level of concentration, such as control (P0) 0 (P1) 12.5 (P2) 25 (P3) 37.5 (P4) 50 (P5) 62.5g. They ware carried out with three repetitions so they could gather 18 experimental units. Providing bean with the banana’s skin waste could give a clear effect towards the plant’s heights, gross weight, and dry weight. Using the banana’s skin waste whice has 50g concentration provides the best resulty for the plant’s height (141.30 cm), the number of leaf (37.67 leaves), and dry weight (12.54 g)

HISTOLOGI LIMPA DAN HEMATOLOGI MENCIT YANG DIINFEKSI Escherichia coli SETELAH PEMBERIAN ASAM HUMAT GAMBUT KALIMANTAN

Spleen Histology and Hematology of Mice Infected by Escherichia coli after Oral Administration of Humic Acid from Borneo PeatABSTRACTHumic acid compounds have an immunostimulatory effect. The aim of this research was to determine the effect of humic acid on the spleen of mice infected with Escherichia coli. The study used a completely randomized design with six treatments and five replicates. The treatments were normal control, negative control, positive control of isoprinosine, humic acid dose of 62.5; 125; and 250 mg/kg body weight (BW). The results showed that E. coli infection caused diarrhea symptoms and significant weight loss. There were significant differences (P<0.05) on hematocrit value and a total leukocyte count of humic acid, in which isoprinosine treatment was higher than those of negative control and normal control. There was no significant difference in the spleen weight of the mice subjected to the different treatments, but through histologic observations a significant difference (P<0.05) was found in the histologic size of the spleen. Humic acid treatment of 250 and 125 mg/kg BW resulted in the widest white pulp (495.8 ± 58.2 µm) and the highest leukocytes count (6725 ± 1018 cell/mL), respectively. On the red pulp serving as negative control numerous clusters of lymphocyte cells were found.Keywords: Escherichia coli, humic acid, peat soil, spleen, white pulp ABSTRAKSenyawa asam humat mempunyai potensi imunostimulan. Penelitian ini bertujuan mengetahui pengaruh pemberian asam humat terhadap organ limpa mencit yang diinfeksi bakteri Escherichia coli. Penelitian ini menggunakan rancangan acak lengkap dengan enam perlakuan dan lima ulangan. Perlakuan tersebut yakni kontrol normal, kontrol negatif, kontrol positif isoprinosin, asam humat dosis 62,5; 125; dan 250 mg/kg berat badan (BB). Hasil penelitian menunjukkan bahwa infeksi bakteri E. coli pada mencit menyebabkan mencit mengalami gejala diare dan penurunan berat badan yang signifikan. Perbedaan signifikan (P<0,05) pada nilai hematokrit dan jumlah leukosit total perlakuan asam humat dan isoprinosin lebih tinggi dibandingkan kontrol negatif dan kontrol normal. Tidak terdapat perbedaan nyata (P>0,05) pada berat limpa mencit antar perlakuan, melalui pengamatan histologi ditemukan perbedaan ukuran histologi limpa mencit. Perlakuan asam humat 250 mg/kg BB mempunyai ukuran pulpa putih (495,8 ± 58,2µm) dan perlakuan asam humat 125 mg/kg BB mempunyai nilai leukosit tertinggi (6725 ± 1018 sel/mL). Pada pulpa merah perlakuan kontrol negatif ditemukan banyak sel limfosit yang menggerombol.Kata Kunci: asam humat, Escherichia coli, limpa, pulpa putih, tanah gambu

ETNOBOTANI TUMBUHAN UPACARA ADAT PERNIKAHAN SUKU MELAYU DI DESA MULIA KERTA KECAMATAN BENUA KAYONG KABUPATEN KETAPANG

Traditional ceremony is a ceremony that carried out from generation to generation, which is related to certain rules based on customs. Ethnobotany in traditional wedding ceremonies is still not widely carried out. The purpose of this study was to find out species of plants, the parts of the plants, the use of plants and the value of the informant agreement ratio of society understanding the plants in the Melayu ethnic wedding ceremony in Mulia Kerta Village. This study was conducted for three months, from February to April 2022 in Mulia Kerta Village, Benua Kayong Sub-district of Ketapang Regency. The research method used the purposive sampling. Quantitative analysis used in this study is to calculating frequency of citation, the use value of each plant part, the ratio of informant agreement, the percentage of plant parts and the percentage of the preparation method. The results showed that the Melayu ethnic in Mulia Kerta Village used 17 plants species that embrace in 15 families for plants in Melayu wedding ceremonies. The most widely used plant parts were leaves as much as 37.6%. The highest utilization value of plants is by boiling, which is 25.3%. The highest citation frequency values were in areca nut (Areca catechu L.), airplant (Kalanchoe pinnata (Lmk) Pers.), pandanus (Pandanus amaryllifolius Roxb.), betle (Piper betle L.), citronella (Cymbopogon winterianus Jowitt) and rice (Oryza sativa L.) as much as 100%. The highest value of informant agreement ratio in the henna stage category using the henna plant (Lawsonia inermis L.) was 1, and the lowest informant agreement ratio value was in the tepung tawar stage category is 0.85

ETNOBOTANI TUMBUHAN PANGAN MASYARAKAT DAYAK KANAYANT DI DESA BABANE KECAMATAN SAMALANTAN KABUPATEN BENGKAYANG KALIMANTAN BARAT

Food plants is a human need to be met at all times. The pattern of utilization of plants in a society is closely related with their culture. Therefore, the culture of a region will determine the type of foods, the plant parts used, and  the way food is proceesed. The utilization of plants is important for the Dayak Kanayant  tribe especially in fulfilling food needs. The study is carried out for 3 months from June to August 2020.  The study aims to identify  the kinds of  food plants, and their use by Dayak Kanayant in Babane Village.  using the snowball method. The data obtained were analyzed descriptively. The results obtained  were 49 species of food plants,  from 31 families, and the most widely used families were moracaceae and aracaceae, 5 species in each of these families with a percentage of  10.20%. The  widely used  portion of the crop are  fruits with a percentage of  38.09%, while the location of food plants is found more in the forest with a percentage of 41.38%. the way to use it is generally consumed directly and some are processed by sauteed, boiled, burned, and fried.The methods of treating vegetation are boiled, dried in the sun, buried in the ground, added salt and sugar

PEMANFAATAN TUMBUHAN PADA UPACARA ANTAR AJONG SUKU MELAYU DI DESA MATANG DANAU KECAMATAN PALOH KABUPATEN SAMBAS

The antar ajong ceremony is a ceremony that is carried out from generation to generation, but the delivery is still orally. The implementation of the ceremony uses many plants to support the stages of the antar ajong ceremony. The purpose of this study was to find out the types of plants used in the antar ajong ceremony, to know the value of the frequency of citations and the use value of the types of plants used in the antar ajong ceremony. This research was conducted from February to July 2022 in Matang Danau Village, Paloh District, Sambas Regency. Methods of determining respondents using the snowball sampling method. The results of the research that has been done obtained as many as 28 species and 18 families of plants that are used in ceremonies between ajong. The most widely used plant family is the Arecaceae family. The highest 100% value of plant citation frequency used in inter-ajong ceremonies consisted of 16 species, namely Areca catechu L., Bambusa sp., Cerbera manghas L., Curcuma longa L., Cocos nucifera L., Cordyline fruticosa (L.) A Chev., Cymbopogon nardus (L.) Rendle., Lygodium microphyllum (Cav.) R. Br., Leea sp. Metroxylon sago Rottb., Musa paradisiaca L., Nicotiana tabacum L., Nypa fruticans Wurmb., Oryza sativa L., Piper betle L., and Stryrax sp. The highest use value of plant species was 0.33 consisting of 3 species, namely Cocos nucifera L., Curcuma longa L., and Oryza sativa L

PRODUKTIVITAS SAWI PAKCHOY (Brassica chinensis L.) PADA TANAH GAMBUT SETELAH PEMBERIAN PUPUK TRICHOKOMPOS KOTORAN BEBEK (Anas Sp.)

Vegetables are one of the foodstuffs that serve as a source of fiber and vegetable protein. However, the process of cultivating vegetables in peat soil is constrained by the high soil acidity and low nutrients in the soil. The study aims to find out the influence and concentration of trichocompost fertilizer duck manure that gives the best results on the productivity of mustard pakchoy (Brassica chinensis L.). The study used a Complete Randomized Design with 5 levels of treatment namely A = 0 (Control); B = 80 g/1,6 Kg; C = 100 g/1,6 Kg; D = 120 g/1,6 Kg; and E = 140 g/1,6 Kg. The data was analyzed using ANOVA and continued Duncan Test with a confidence level of 95%. The results showed that the aplication of trichocompost fertilizer duck manure has a significant effect on the coarse protein content and coarse fiber content, but has no noticeable effect on chlorophyll content. Trichocompost fertilizer treatment 140 g/1,6 Kg is the treatment that gives the best results to the productivity of pakchoy plants for the parameters of crude fiber content (2,540%), while the highest yield on coarse protein parameters was found in the control (3,636%)

ETNOBOTANI PEMANFAATAN TUMBUHAN DALAM RITUAL ADAT NYELEPAT TAUN SUKU DAYAK DE’SA DI KECAMATAN SEPAUK KABUPATEN SINTANG

This Study aims to investigate the species of plants, plant parts, plant usage, and their meanings in the traditional ritual of Nyelepat Taun. The research was conducted from April to August 2022 in Sepauk Subdistrict, Sintang Regency, using the qualitative descriptive method and snowball sampling technique. Participatory observation, structured interviews, and documentation were used for data collection. The findings revealed that the Nyelepat Taun ritual utilized 13 species of plants across 8 families, with leaves being the most frequently used part (31%) and the majority obtained from the yard (38%). The plants with the highest citation frequency were Manihot esculenta (cassava), Oryza sativa (paddy rice), Oryza glutinosa (glutinous rice), Areca cathechu (betel nut), Piper betle (betel leaf), and Vitex pinnata (West indian Lavender) (100%), whereas Imperata cylindrica (cogon grass) had the lowest citation frequency (69%). The most common usage method was direct consumption without processing (54%). The ritual's significance was to symbolize simplicity, long life expectancy, fertility of future lands, abundant blessings, peaceful hearts, prevention of misfortune, and hope for a bountiful crop

AKTIVITAS ANTIFUNGI EKSTRAK METANOL DAUN SALAM (Syzygium polyanthum [Wight] Walp.) TERHADAP PERTUMBUHAN Malassezia sp. (M1) SECARA IN VITRO

Malassezia sp. is a normal flora on the skin of humans and animals. A considerable amount of the fungus may cause tinea versicolor and dandruff. One of the plants that is known to have antifungal compounds is bay leaf (Syzygium polyanthum [Wight] Walp.). This research was done to determine the ability and the Minimum Inhibitory Concentration (MIC) of methanol extract from bay leaves S. polyanthum in inhibiting the growth of Malassezia sp. (M1). This study used a completely randomized design (CRD) with  treatment levels that consisted of concentrations of 80%, 90%, and 100%, negative control (sterile distilled water) and positive control (ketoconazole 2%). The antifungal activity test was carried out using the Kirby-Bauer method. The data showed that each treatment levels had different results. Concentrations of 90% and 100%, and positive control showed inhibition zones of 9.27 mm, 11.57 mm, and 16.67 mm, respectively. Therefore the concentration of 90% was the Minimum Inhibitory Concentration (MIC) of methanol extract from bay leaves S. polyanthum against the growth of Malassezia sp. (M1)

KEPADATAN DAN POLA PENYEBARAN Shorea leprosula Miq. DI STASIUN PENELITIAN CABANG PANTI TAMAN NASIONAL GUNUNG PALUNG KALIMANTAN BARAT

Shorea leprosula has high economic and ecological value. Shorea leprosula population decreases due to increased illegal logging activities, especially in Gunung Palung National Park. This research aims to find out the density and spreading pattern of Shorea leprosula Miq. at Research Stasiun Cabang Panti, Gunung Palung National Park, West Kalimantan. The research was conducted from June to August 2020. The research method used is random sampling in 3 different locations, namely alluvial habitats, sandy rocks and freshwater swamps. Sampling using a squared method with a plot size of 20 x 20 m and made 10 plots on each habitat. The results showed that the density of S. leprosula is highest in freshwater swamp habitat of 170 individuals/Ha and the lowest in sandy rock habitat of 103 individuals/Ha. Spreading pattern S. leprosula is aggregate