Internal Calibration Potentiometric Aptasensors for Simultaneous Detection of Hg²⁺, Cd²⁺, and As³⁺ Based on a Screen-Printed Carbon Electrodes Array

An all-solid-state potentiometric aptasensor array based on a multichannel disposable screen-printed carbon electrode (SPCE) was demonstrated for the simultaneous detection of Hg²⁺, Cd²⁺, and As³⁺ by open circuit potential (OCP) technology. The potential of the channel with an internal calibration DNA sequence (IC-DNA) was employed as the internal calibration potential (ICP) to subtract the background signal generated by the detection system, providing a built-in correction methodology. As a result, the developed aptasensor array showed high sensitivity and accuracy for detecting Hg²⁺, Cd²⁺, and As³⁺ without mutual interference or interference from other ions. The linear response ranged from 2.5 pM to 2.5 μM, and the detection limits for Hg²⁺, Cd²⁺, and As³⁺ were 2.0, 0.62, and 0.17 pM, respectively. Furthermore, the potentiometric aptasensor array was successfully applied for the simultaneous detection of three ions in real samples. The results obtained from the developed approach agreed well with the results obtained from inductively coupled plasma mass spectrometry

Additional file 3 of Multiple network algorithm for epigenetic modules via the integration of genome-wide DNA methylation and gene expression data

Supplementary Figure S3. Breast cancer subtype prediction: (A) accuracy of compared algorithms using a 3-fold cross validation, (B) accuracy of compared algorithms using a 10-fold cross validation, and (C) feature importance of positively and negatively correlated modules in classifying the breast cancer subtypes. (EPS 839 kb