Performance evaluation of induced mutant lines of black gram (Vigna mungo (L.) Hepper)

Article Details: Received: 2020-01-12      |      Accepted: 2020-03-02      |      Available online: 2020-06-30https://doi.org/10.15414/afz.2020.23.02.70-77 Present investigation was carried out to explore the possibility of inducing genetic variability for yield and yield contributing traits in well-adapted variety PU-19 of black gram (Vigna mungo (L.) Hepper) following mutagenesis with methyl methane sulfonate (MMS), sodium azide (SA) and hydrazine hydrate (HZ). A considerable increase in mean values for fertile branches per plant, pods per plant and total plant yield was noticed among the mutant lines in M4 and M5 generations. Estimates of genotypic coefficient of variation, heritability and genetic advance for yield and yield components were also recorded to be higher compared to control. MMS followed by SA and HZ showed highest mutagenic potential for improving total plant yield of black gram var. PU-19. Treatment concentration 0.3% was found to be most effective in generating significant increase in total plant yield of black gram var. PU-19. The increased genetic variability for yield and yield components indicates the ample scope of selection for superior mutants in subsequent generations due to preponderance of additive gene action.Keywords: black gram, mutagenesis, chemical mutagens, genetic variability, yield componentsReferences AHLOOWALIA, B., MALUSZYNSKI, M. and NICHTERLEIN, K.(2004). Global impacts of mutation derived varieties. Euphytica, 135, 187. ANNUAL REPORT (2016–2017). In: Government of India, Ministry of Agriculture and Farmers Welfare, Department of Agriculture, Cooperation and Farmers Welfare, Directorate of Pulses Development, Vindhyachal Bhavan, India. AUTI, S. G. (2012). Induced morphological and quantitative mutants in mungbean. Biorem. Biodiv. Bioavail., 6 (Special Issue), 27-39. BHATIA, C. R. and SWAMINATHAN, M. S. (1962). 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Comparative Mutagenic Effectiveness and Efficiency of Gamma Rays and Sodium Azide in Inducing Chlorophyll and Morphological Mutants of Cowpea

Mutagenic effectiveness and efficiency are the most important factors determining the success of mutation breeding, a coherent tool for quickly enhancing genetic diversity in crops. However, conclusive evidence of using an effective and efficient dose of gamma (γ) rays and sodium azide (SA) for genetic improvement is scant. The present study assesses genetic diversity in M2 mutants of cowpea and evaluates mutagenic effectiveness and efficiency of the single and combination doses of γ rays and SA. In M0 generation, 7200 M1 seeds obtained by SA treatment (0.01—0.1%) and γ irradiation (100—1000 Gy) at a dose rate of 11.58 Gy/min were sown to raise M1 generation. A total of 57,620 M2 seeds were generated from the M1 generation of two varieties—Gomati VU-89 and Pusa-578, from which 47,650 seeds germinated. Moreover, plants (38,749) that survived were screened for chlorophyll and morphological mutations. Among the mutagens, SA followed by γ rays + SA and γ rays was most effective in inducing higher frequency and a broader spectrum of chlorophyll mutants. A wide range of morphological mutants affecting every growth stage was recorded with the highest frequency in 400 Gy γ rays + 0.04% SA treatment. These morphological mutants with desirable agronomic traits represent a valuable genetic resource for future breeding programs. This study revealed the potency of γ rays and SA in increasing genetic diversity and demonstrated the successful conduct of induced mutagenesis in the cowpea

Barium Enema in the Diagnosis of Hirschsprung's Disease: A Comparison with Rectal Biopsy

Aim: The aim of the present study was to compare the diagnostic accuracy of a barium enema in Hirschsprung's disease with respect to a rectal biopsy. Materials And Methods: This study was conducted on 60 consecutive patients of clinically suspected cases of Hirschsprung's disease admitted to a tertiary care hospital in Kashmir over a period of three and a half years. All the patients were subjected to a barium enema and a full-thickness rectal biopsy. Results: The mean age was 3.5 + 0.5 years. A rectal biopsy revealed absence of ganglion cells in 36 (60%) patients, absence of ganglion cells with nerve hypertrophy in 4 (6.66%) patients and ruled out Hirschsprung's disease in 20 (33.33%) patients. A barium enema was compatible with Hirschsprung's disease in 20 (33.33%), suggestive in 14 (23.33%) and unremarkable in 26 (43.33%) patients. A barium enema had a diagnostic accuracy of 76.67% with a positive predictive value of 88.23%, a negative predictive value of 61.53%, specificity of 80% and a sensitivity of 75%, and a rectal biopsy had a diagnostic accuracy of 100% (P value < 0.0001). Conclusion: Barium enema is a valuable diagnostic tool for the diagnosis of patients with Hirschsprung's disease. We recommend a barium enema as a routine initial workup in the management of patients of suspected Hirschsprung's disease. [Arch Clin Exp Surg 2013; 2(4.000): 224-228

Secondary Metabolite Profiling, Anti-Inflammatory and Hepatoprotective Activity of Neptunia triquetra (Vahl) Benth

The present study aimed to analyze the phytoconstituents of Neptunia triquetra (Vahl) Benth. Anti-inflammatory and hepatoprotective activities of ethanol (EE), chloroform (CE) and dichloromethane (DCME) of stem extracts were evaluated using in vivo experimental models. The extracts were analyzed for phytoconstituents using GC-HRMS. Anti-inflammatory activity of CE, EE and DCME was accessed using carrageenan-induced paw oedema, cotton pellet-induced granuloma and the carrageenan-induced air-pouch model in Wistar albino rats. The hepatotoxicity-induced animal models were investigated for the biochemical markers in serum (AST, ALT, ALP, GGT, total lipids and total protein) and liver (total protein, total lipids, GSH and wet liver weight). In the in vivo study, animals were divided into different groups (six in each group) for accessing the anti-inflammatory and hepatoprotective activity, respectively. GC-HRMS analysis revealed the presence of 102 compounds, among which 24 were active secondary metabolites. In vivo anti-inflammatory activity of stem extracts was found in the order: indomethacin &gt; chloroform extract (CE) &gt; dichloromethane extract (DCME) &gt; ethanolic extract (EE), and hepatoprotective activity of stem extracts in the order: CE &gt; silymarin &gt; EE &gt; DCME. The results indicate that N. triquetra stem has a higher hepatoprotective effect than silymarin, however the anti-inflammatory response was in accordance with or lower than indomethacin