565 research outputs found
Co-interest Person Detection from Multiple Wearable Camera Videos
Wearable cameras, such as Google Glass and Go Pro, enable video data
collection over larger areas and from different views. In this paper, we tackle
a new problem of locating the co-interest person (CIP), i.e., the one who draws
attention from most camera wearers, from temporally synchronized videos taken
by multiple wearable cameras. Our basic idea is to exploit the motion patterns
of people and use them to correlate the persons across different videos,
instead of performing appearance-based matching as in traditional video
co-segmentation/localization. This way, we can identify CIP even if a group of
people with similar appearance are present in the view. More specifically, we
detect a set of persons on each frame as the candidates of the CIP and then
build a Conditional Random Field (CRF) model to select the one with consistent
motion patterns in different videos and high spacial-temporal consistency in
each video. We collect three sets of wearable-camera videos for testing the
proposed algorithm. All the involved people have similar appearances in the
collected videos and the experiments demonstrate the effectiveness of the
proposed algorithm.Comment: ICCV 201
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A Targeted Quantitative Proteomic Method Revealed a Substantial Reprogramming of Kinome during Melanoma Metastasis.
Kinases are involved in numerous critical cell signaling processes, and dysregulation in kinase signaling is implicated in many types of human cancers. In this study, we applied a parallel-reaction monitoring (PRM)-based targeted proteomic method to assess kinome reprogramming during melanoma metastasis in three pairs of matched primary/metastatic human melanoma cell lines. Around 300 kinases were detected in each pair of cell lines, and the results showed that Janus kinase 3 (JAK3) was with reduced expression in the metastatic lines of all three pairs of melanoma cells. Interrogation of The Cancer Genome Atlas (TCGA) data showed that reduced expression of JAK3 is correlated with poorer prognosis in melanoma patients. Additionally, metastatic human melanoma cells/tissues exhibited diminished levels of JAK3 mRNA relative to primary melanoma cells/tissues. Moreover, JAK3 suppresses the migration and invasion of cultured melanoma cells by modulating the activities of matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9). In summary, our targeted kinome profiling method provided by far the most comprehensive dataset for kinome reprogramming associated with melanoma progression, which builds a solid foundation for examining the functions of other kinases in melanoma metastasis. Moreover, our results reveal a role of JAK3 as a potential suppressor for melanoma metastasis
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