A novel expression cassette for the efficient visual selection of transformed tissues in florists' chrysanthemum (Chrysanthemum morifolium Ramat.).

Constructs carrying visual reporter genes coupled with efficient promoters could facilitate the process of identification and selection of stable transformants in recalcitrant crops. Here, a novel construct utilizing a ribulose-1,5-bisphosphate carboxylase (RbcS) promoter combined with the green fluorescent protein (GFP) reporter gene to initiate very high expression of GFP in florist's chrysanthemum (Chrysanthemum morifolium Ramat.) was described. Based on this expression cassette, a new regeneration protocol using leaf discs as explants was developed for the Agrobacterium-mediated transformation of Chrysanthemum genotype ‘1581’, and a transformation efficiency of 7% was obtained. The expression of two different GFP constructs targeted to either cytosol or plastids was compared in transgenic lines. Both GFP constructs were expressed at such a high level that the green fluorescence dominated red fluorescence in the leaf tissues, allowing easy observation and microdissection of transformed tissues even without a GFP filter. Under normal light, plants with GFP targeted to plastids had a light green phenotype deriving from the high GFP expression. Quantitative reverse transcriptional PCR analysis showed that the plastid targeted construct with intron had significantly higher steady state transcript levels of GFP mRNA. This novel expression cassette may allow direct visual selection of transformed tissues independent of antibiotic selection in a wide range of plant specie

Information theoretic analysis of LSD scheme

In this paper, the capacity region of Low Density Signature Multiple Access Channel (LDS-MAC) is calculated through information theoretic analysis. LDS Code Division Multiple Access (LDS-CDMA) uses spreading sequences of low density for spreading the data symbols in time domain. This technique benefits from a less complex Multiuser Detector (MUD) compared to conventional CDMA with optimum MUD; while keeping the performance close to the single user scenario for up to 200% loaded conditions. Also evaluated is the effect of different factors on the capacity of LDS MAC

Analysis of the scalar doubly heavy tetraquark states with QCD sum rules

In this article, we perform a systematic study of the mass spectrum of the scalar doubly charmed and doubly bottom tetraquark states using the QCD sum rules.Comment: 17 pages, 24 figures, add more discussion

On the Harmonic approximation for large Josephson junction coupling charge qubits

We revisit the harmonic approximation (HA) for a large Josephson junction interacting with some charge qubits through the variational approach for the quantum dynamics of the junction-qubit coupling system. By making use of numerical calculation and analytical treatment, the conditions under which HA works well can be precisely presented to control the parameters implementing the two-qubit quantum logical gate through the couplings to the large junction with harmonic oscillator (HO) Hamiltonian.Comment: 7 pages, 3 figure

Shoot organogenesis in leaf explants of Hydrangea macrophylla ‘Hyd1’ and assessing genetic stability of regenerants using ISSR markers

For the first time, an in vitro regeneration protocol of Hydrangea macrophylla 'Hyd1' was developed. Effects of different plant growth regulators (PGRs) on shoot regeneration were investigated jointly with selecting optimal basal media and cefotaxime concentrations. The highest frequency of shoot organogenesis (100%) and mean number of shoots per explant (2.7) were found on Gamborg B5 basal medium supplemented with 2.25 mg/l 6-benzyladenine (BA), 0.1 mg/l Indole-3-butyric acid (IBA), 100 mg/l cefotaxime and 30 g/l sucrose solidified by 7 g/l agar. Regenerated shoots were rooted by culturing on perlite plus half strength liquid B5 basal medium with 0.5 mg/l NAA. Rooted plantlets were transplanted to the greenhouse with 100% survival rate. Genetic stability of 32 plantlets (one mother plant and 31 regenerants) was assessed by 44 ISSR markers. Out of 44 ISSR markers, ten markers produced clear, reproducible bands with a mean of 5.9 bands per marker. The in vitro regeneration protocol is potentially useful for the genetic transformation of Hydrangea macrophylla 'Hyd1'

Analysis of the vertexes ΞQ∗ΞQ′V\Xi_Q^*\Xi'_Q V, ΣQ∗ΣQV\Sigma_Q^*\Sigma_Q V and radiative decays ΞQ∗→ΞQ′γ\Xi_Q^*\to \Xi'_Q \gamma, ΣQ∗→ΣQγ\Sigma_Q^*\to \Sigma_Q \gamma

In this article, we study the vertexes $\Xi_Q^*\Xi'_Q V$ and $\Sigma_Q^* \Sigma_Q V$ with the light-cone QCD sum rules, then assume the vector meson dominance of the intermediate $\phi(1020)$, $\rho(770)$ and $\omega(782)$, and calculate the radiative decays $\Xi_Q^*\to \Xi'_Q \gamma$ and $\Sigma_Q^*\to \Sigma_Q \gamma$.Comment: 28 pages, 4 tables, revised versio

Maximal Entanglement of Two-qubit States Constructed by Linearly Independent Coherent States

In this paper, we find the necessary and sufficient condition for the maximal entanglement of the state, $|\psi>=\mu|\alpha>|\beta>+\lambda|\alpha>|\delta>+ \rho|\gamma>|\beta>+\nu|\gamma>|\delta>,$ constructed by linearly independent coherent states with \emph{real parameters} when $=$. This is a further generalization of the classified nonorthogonal states discussed in Ref. Physics Letters A {\bf{291}}, 73-76 (2001).Comment: some examples added; Int J Theor Phys 201

Impulse-driven surface breakdown data : a Weibull statistical analysis

Surface breakdown of oil-immersed solids chosen to insulate high-voltage, pulsed-power systems is a problem that can lead to catastrophic failure. Statistical analysis of the breakdown voltages, or times, associated with such liquid-solid interfaces can reveal useful information to aid system designers in the selection of solid materials. Described in this paper are the results of a Weibull statistical analysis, applied to both breakdown-voltage data and time-to-breakdown data generated in gaps consisting of five different solid polymers immersed in mineral oil. Values of the location parameter γ provide an estimate of the applied voltage below which breakdown will not occur, and under uniform-field conditions, γ varied from 192 kV (480 kV/cm) for polypropylene to zero for ultra-high molecular weight polyethylene. Longer times to breakdown were measured for UHMWPE when compared with the other materials. However, high values of the shape parameter β reported in the present paper suggest greater sensitivity to an increase in applied voltage – that is, the probability of breakdown increases more sharply with increasing applied voltage for UHMWPE compared to the other materials. Analysing peak-applied-voltage data, only PP consistently reflected a low value of β across the different sets of test conditions. In general, longer mean times to breakdown were found for solid materials of εr more closely matched to that of the surrounding mineral oi

The Legal Organization of Administrative Responsibility in the Palestinian POLICE: In Accordance with Palestinian POLICE Decree No. (23) of 2017

This study seeks to clarify the administrative system of administrative accountability in the Palestinian POLICE, by clarifying the structural and procedural structure to arrive at an administrative identification, and we will work on clarifying the strengths in the POLICE decree and on clarifying the deficiencies in the Palestinian POLICE decree, We will explain what the obstacles that hindered the progress of the Palestinian POLICE are and how the Palestinian POLICE overcame and excelled from an administrative point of view, The study did not stop there. It addressed aspects of administrative grievance and administrative appeals against administrative decisions of workers in the Palestinian POLICE. It explained these procedures in line with the modern administrative vision of the Palestinian POLICE, which aims to raise the administrative system in the POLICE to the highest level of transparency and integrity following the principle Equality according to international standards. Keywords: Administrative law, administrative responsibility, disciplinary violations, behavioral violations, administrative decision, administrative appeal, Palestinian POLICE. DOI: 10.7176/JLPG/96-12 Publication date: April 30th 202

Pulsed light beams in vacuum with superluminal and negative group velocities

Gouy's phase of transversally limited pulses can create a strong anomalous dispersion in vacuum leading to highly superluminal and negative group velocities. As a consequence, a focusing pulse can diverge beyond the focus before converging into it. A simple experiment is proposed.Comment: 4 pages, 5 figure