149,029 research outputs found
The effects of dog management on Echinococcus spp. prevalence in villages on the eastern Tibetan Plateau, China
Background The pastoral area of the eastern Tibetan plateau is a very important human echinococcosis endemic region. Domestic dogs are the main definitive host for the transmission of Echinococcus granulosus sensu lato (s.1.) and E. multilocularis to humans. To control the infection risks, a national-level canine echinococcosis prevention and control program has been implemented since 2015 in Shiqu County, Sichuan, China, The objective of this investigation was to evaluate its effect on Echinococcus spp. prevalence in dogs. Methods We surveyed 69 households with 84 owned dogs, for dog keeping information in the villages of Rizha and Eduoma. A total of 105 dog fecal samples, consisting of 75 from owned dogs and 30 unknown dog fecal samples were collected between 2015 and 2017 to determine Echinococcus spp. prevalence using copro-PCR. Eight variables based on household surveys were included into a logistic regression model for significantly relevant factors to canine echinococcosis prevalence in dogs. Results The overall Echinococcus spp. copro-DNA prevalence decreased significantly in dogs from 51.2% (2015) to 20.0% (2017) in Rizha, and insignificantly from 11.5% (2016) to 4.3% (2017) in Eduoma. Echinococcus multilocularis was the most prevalent species continually detected during the entire research period, while E. granulosus was rare and not detected in 2017. Echinococcus shiquicus prevalence was as high as E. multilocularis , although only detected in 2015 in Rizha. Unleashed dog feces were mainly collected in Rizha Village in 2015. Although 93.2% of owned dogs were leashed, and the monthly praziquantel dosing rate reached 97%, E. multilocularis infection could still be detected in 11.1% of owned dogs in 2017. Monthly deworming, leashing dogs 24h per day, and the avoidance of dogs feeding on livestock viscera are significant measures to prevent canine echinococcosis infection in owned dogs. Conclusion Carrying out a canine echinococcosis prevention and control program can significantly decrease the Echinococcus prevalence. The potential contact between leashed dogs and wild small mammals is still a risk to re-infect owned dogs. This study shows that the long term application of regular dog dosing in the vast remote echinococcosis endemic areas of west China is still challenging
Geometric phases induced in auxiliary qubits by many-body systems near its critical points
The geometric phase induced in an auxiliary qubit by a many-body system is
calculated and discussed. Two kinds of coupling between the auxiliary qubit and
the many-body system are considered, which lead to dephasing and dissipation in
the qubit, respectively. As an example, we consider the XY spin-chain
dephasingly couple to a qubit, the geometric phase induced in the qubit is
presented and discussed. The results show that the geometric phase might be
used to signal the critical points of the many-body system, and it tends to
zero with the parameters of the many-body system going away from the critical
points
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China's energy consumption in the building sector: A Statistical Yearbook-Energy Balance Sheet based splitting method
China's energy consumption in the building sector (BEC) is not counted as a separate type of energy consumption, but divided and mixed in other sectors in China's statistical system. This led to the lack of historical data on China's BEC. Moreover, previous researches' shortages such as unsystematic research on BEC, various estimation methods with complex calculation process, and difficulties in data acquisition resulted in “heterogeneous” of current BEC in China. Aiming to these deficiencies, this study proposes a set of China building energy consumption calculation method (CBECM) by splitting out the building related energy consumption mixed in other sectors in the composition of China Statistical Yearbook-Energy Balance Sheet. Then, China's BEC from 2000 to 2014 are estimated using CBECM and compared with other studies. Results show that, from 2000 to 2014, China's BEC increased 1.7 times, rising from 301 to 814 million tons of standard coal consumed, with the BEC percentage of total energy consumption stayed relatively stable between 17.7% and 20.3%. By comparison, we find that our results are reliable and the CBECM has the following advantages over other methods: data source is authoritative, calculation process is concise, and it is easy to obtain time series data on BEC etc. The CBECM is particularly suitable for the provincial government to calculate the local BEC, even in the circumstance with statistical yearbook available only
A rapid staining-assisted wood sampling method for PCR-based detection of pine wood nematode Bursaphelenchus xylophilus in Pinus massoniana wood tissue
For reasons of unequal distribution of more than one nematode species in wood, and limited
availability of wood samples required for the PCR-based method for detecting pinewood nematodes in
wood tissue of Pinus massoniana, a rapid staining-assisted wood sampling method aiding PCR-based
detection of the pine wood nematode Bursaphelenchus xylophilus (Bx) in small wood samples of P.
massoniana was developed in this study. This comprised a series of new techniques: sampling, mass
estimations of nematodes using staining techniques, and lowest limit Bx nematode mass determination
for PCR detection. The procedure was undertaken on three adjoining 5-mg wood cross-sections, of
0.5 · 0.5 · 0.015 cm dimension, that were cut from a wood sample of 0.5 · 0.5 · 0.5 cm initially, then
the larger wood sample was stained by acid fuchsin, from which two 5-mg wood cross-sections (that
adjoined the three 5-mg wood cross-sections, mentioned above) were cut. Nematode-staining-spots
(NSSs) in each of the two stained sections were counted under a microscope at 100· magnification. If
there were eight or more NSSs present, the adjoining three sections were used for PCR assays. The
B. xylophilus – specific amplicon of 403 bp (DQ855275) was generated by PCR assay from 100.00% of
5-mg wood cross-sections that contained more than eight Bx NSSs by the PCR assay. The entire
sampling procedure took only 10 min indicating that it is suitable for the fast estimation of nematode
numbers in the wood of P. massonina as the prelimary sample selections for other more expensive
Bx-detection methods such as PCR assay
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