Bronchoalveolar Lavage Fluid-Derived Exosomes: A Novel Role Contributing to Lung Cancer Growth

Exosomes are nanovesicles produced by a number of different cell types and regarded as important mediators of cell-to-cell communication. Although bronchoalveolar lavage fluid (BALF) has been shown to be involved in the development of tumors, its role in lung cancer (LC) remains unclear. In this article, we systemically studied BALF-derived exosomes in LC. C57BL/6 mice were injected with Lewis lung carcinoma cells and exposed to non-typeable Haemophilus influenza (NTHi) lysate. The analysis showed that the growth of lung tumors in these mice was significantly enhanced compared with the control cohort (only exposure to air). Characterization of the exosomes derived from mouse BALF demonstrated elevated levels of tumor necrosis factor alpha and interleukin-6 in mice exposed to NTHi lysates. Furthermore, abnormal BALF-derived exosomes facilitated the development of LC in vitro and in vivo. The internalization of the BALF-derived exosomes contributed to the development of LC tumors. Collectively, our data demonstrated that exosomes in BALF are a key factor involved in the growth and progression of lung cancer

Geometry-Based Distributed Spatial Skyline Queries in Wireless Sensor Networks

Algorithms for skyline querying based on wireless sensor networks (WSNs) have been widely used in the field of environmental monitoring. Because of the multi-dimensional nature of the problem of monitoring spatial position, traditional skyline query strategies cause enormous computational costs and energy consumption. To ensure the efficient use of sensor energy, a geometry-based distributed spatial query strategy (GDSSky) is proposed in this paper. Firstly, the paper presents a geometry-based region partition strategy. It uses the skyline area reduction method based on the convex hull vertices, to quickly query the spatial skyline data related to a specific query area, and proposes a regional partition strategy based on the triangulation method, to implement distributed queries in each sub-region and reduce the comparison times between nodes. Secondly, a sub-region clustering strategy is designed to group the data inside into clusters for parallel queries that can save time. Finally, the paper presents a distributed query strategy based on the data node tree to traverse all adjacent sensors’ monitoring locations. It conducts spatial skyline queries for spatial skyline data that have been obtained and not found respectively, so as to realize the parallel queries. A large number of simulation results shows that GDSSky can quickly return the places which are nearer to query locations and have larger pollution capacity, and significantly reduce the WSN energy consumption

Comparison of read and write latency of Level DB before modification.

Comparison of read and write latency of Level DB before modification.</p

Function-Aware Anomaly Detection Based on Wavelet Neural Network for Industrial Control Communication

Function control, which is an essential link in industrial automation, is undergoing a growing integration with ICTs (Information Communication Technologies) because of the flexible manufacturing and convenient interoperability in CPSs (Cyber-Physical Systems). However, it has also brought the increasing dangers of cyberattacks caused by malicious or intentional industrial process control exploitations. In order to effectively detect these cyber intrusions and anomalies, this paper proposes a function-aware anomaly detection approach based on WNN (Wavelet Neural Network), which perceives the abnormal function control changes in industrial control communication. By appropriately extracting the time-related function control characteristics from industrial communication packets, this approach builds an optimized wavelet neural network to model the normal function control behaviors and calculates the detection threshold to differentiate the aberrant industrial process control activities. Additionally, a real-world control system, whose communication protocol is Modbus/TCP, is simulated to furnish the analyzed function control data. According to the experimental results, we fully demonstrate this approach has the fine detection accuracy and adequate real-time capability

Comparison of read and write latency of Level DB after modification.

Comparison of read and write latency of Level DB after modification.</p

Off-chain Level DB storage model architecture diagram.

Off-chain Level DB storage model architecture diagram.</p

Attribute fields contained in Super Block.

In order to foster a modern economic system and facilitate high-quality economic development, it is crucial to establish a conducive business environment. Undoubtedly, the evaluation of the business environment for enterprises constitutes a prominent area of research. Nevertheless, ensuring the authenticity and security of the raw data sources provided by participating enterprises poses a challenge, thereby compromising the accuracy of the evaluation. To tackle this issue, an enterprise composite blockchain construction method for business environment is proposed in this paper, which stores the raw data of enterprises by the means of hybrid on-chain and off-chain. Initially, the enhanced hash function SHA256 is introduced to encrypt the raw data of enterprises. The encrypted data is subsequently stored in an off-chain Level DB database, which is based on non-volatile memory. This approach effectively alleviates the burden on communication and storage. Secondly, a composite storage strategy on-chain is adopted: the key values from the Level DB are stored in the DAG-based Conflux public blockchain, while the enterprise state data is stored in the consortium blockchain, so as to provide trusted evidence of business environment evaluation data. Finally, it is demonstrated through a large number of experimental comparisons that the enterprise composite blockchain construction method proposed in this paper exhibits better read and write performance, lower storage efficiency and storage overhead, and outperforms both the before-improved Level DB database and existing blockchain storage models.</div

Symbols and their meanings.

In order to foster a modern economic system and facilitate high-quality economic development, it is crucial to establish a conducive business environment. Undoubtedly, the evaluation of the business environment for enterprises constitutes a prominent area of research. Nevertheless, ensuring the authenticity and security of the raw data sources provided by participating enterprises poses a challenge, thereby compromising the accuracy of the evaluation. To tackle this issue, an enterprise composite blockchain construction method for business environment is proposed in this paper, which stores the raw data of enterprises by the means of hybrid on-chain and off-chain. Initially, the enhanced hash function SHA256 is introduced to encrypt the raw data of enterprises. The encrypted data is subsequently stored in an off-chain Level DB database, which is based on non-volatile memory. This approach effectively alleviates the burden on communication and storage. Secondly, a composite storage strategy on-chain is adopted: the key values from the Level DB are stored in the DAG-based Conflux public blockchain, while the enterprise state data is stored in the consortium blockchain, so as to provide trusted evidence of business environment evaluation data. Finally, it is demonstrated through a large number of experimental comparisons that the enterprise composite blockchain construction method proposed in this paper exhibits better read and write performance, lower storage efficiency and storage overhead, and outperforms both the before-improved Level DB database and existing blockchain storage models.</div

NVMTable format diagram.

In order to foster a modern economic system and facilitate high-quality economic development, it is crucial to establish a conducive business environment. Undoubtedly, the evaluation of the business environment for enterprises constitutes a prominent area of research. Nevertheless, ensuring the authenticity and security of the raw data sources provided by participating enterprises poses a challenge, thereby compromising the accuracy of the evaluation. To tackle this issue, an enterprise composite blockchain construction method for business environment is proposed in this paper, which stores the raw data of enterprises by the means of hybrid on-chain and off-chain. Initially, the enhanced hash function SHA256 is introduced to encrypt the raw data of enterprises. The encrypted data is subsequently stored in an off-chain Level DB database, which is based on non-volatile memory. This approach effectively alleviates the burden on communication and storage. Secondly, a composite storage strategy on-chain is adopted: the key values from the Level DB are stored in the DAG-based Conflux public blockchain, while the enterprise state data is stored in the consortium blockchain, so as to provide trusted evidence of business environment evaluation data. Finally, it is demonstrated through a large number of experimental comparisons that the enterprise composite blockchain construction method proposed in this paper exhibits better read and write performance, lower storage efficiency and storage overhead, and outperforms both the before-improved Level DB database and existing blockchain storage models.</div

Level DB read and write performance test environment.

Level DB read and write performance test environment.</p