MiR 206 inhibits reorganization of the cytoskeleton in melanoma cells by targeting DDX5

Purpose: To investigate the role and mechanism of microRNA-206 (miR-206) in cytoskeleton reorganization in melanoma cells. Methods: MiR-206 and RNA helicase p68 (DDX5) expression levels were measured in A375, A875, and HEM-M cells by quantitative real time polymerase chain reaction (qRT-PCR). A DDX5 overexpression cell line was constructed, and DDX5 overexpression, A375, and A875 cells were transfected with miR-206 mimic or DDX5 small interfering RNA (siRNA). Transwell assay was used to assess cell migration and invasion of A375 and A875 cells, while Luciferase reporter assay was used to determine the putative target of miR-206. DDX5, miR-206, vinculin, coronin3, and ezrin expression levels were evaluated by qRT-PCR. Protein expressions of DDX5, vinculin, coronin3, and ezrin were evaluated by western blot analysis. Results: DDX5 expression was higher and miR-206 expression lower in A375 and A875 cells when compared to HEM-M cells (p < 0.05). Knockdown of DDX5 and overexpression of miR-206 repressed invasion and migration, and inhibited expression of vinculin, coronin3, and ezrin in A375 and A875 cells (p < 0.05). However, overexpression of DDX5 reversed the effect of miR-206 on cytoskeletal protein expression. Luciferase reporter assay data confirmed that DDX5 is a direct target of miR-206 (p < 0.05). Conclusion: MiR-206 suppresses reorganization of the cytoskeleton in melanoma cells by targeting DDX5, and is thus, a promising target for the treatment of melanoma

6-Amino-8-(2-bromo­phen­yl)-1,7,8,8a-tetrahydro-3H-isothio­chromene-5,7,7-tricarbonitrile dimethyl­formamide solvate

In the title compound, C18H13BrN4S·C3H7NO, the thio­pyran ring and the adjacent six-numbered ring adopt distorted boat conformations. The mol­ecules, lying about inversion centers, form hydrogen-bonded dimers involving one of the H atoms on the amino group with the N atom of a cyano group of an adjacent mol­ecule, resulting in a 12-membered ring system [R 2 2(12) ring motif]. The other H atom of the amino group forms an inter­molecular hydrogen bond with the O atom of the dimethyl­formamide (DMF) mol­ecule. Another lone pair of electrons on the same carbonyl O atom of DMF mol­ecule forms a non-classical C—H⋯O inter­molecular hydrogen bond, resulting in a chain of mol­ecules

DragonDiffusion: Enabling Drag-style Manipulation on Diffusion Models

Despite the ability of existing large-scale text-to-image (T2I) models to generate high-quality images from detailed textual descriptions, they often lack the ability to precisely edit the generated or real images. In this paper, we propose a novel image editing method, DragonDiffusion, enabling Drag-style manipulation on Diffusion models. Specifically, we construct classifier guidance based on the strong correspondence of intermediate features in the diffusion model. It can transform the editing signals into gradients via feature correspondence loss to modify the intermediate representation of the diffusion model. Based on this guidance strategy, we also build a multi-scale guidance to consider both semantic and geometric alignment. Moreover, a cross-branch self-attention is added to maintain the consistency between the original image and the editing result. Our method, through an efficient design, achieves various editing modes for the generated or real images, such as object moving, object resizing, object appearance replacement, and content dragging. It is worth noting that all editing and content preservation signals come from the image itself, and the model does not require fine-tuning or additional modules. Our source code will be available at https://github.com/MC-E/DragonDiffusion

An Experimental Investigation on the Failure Behavior of a Notched Concrete Beam Strengthened with Carbon Fiber-Reinforced Polymer

This paper presents an experiment investigation on the failure behavior of a notched concrete beam reinforced with CFRP, by exploring the influences of the length, thickness, and CFRP bonding methods on the ultimate bearing capacity and failure mode. The interfacial shear stress has first been analytically derived and parametric analyses are then made to predict the failure mode. The experiment observation finds that failure mode significantly depends on CFRP length. The brittle fracture occurs only for nonstrengthened beams; the shear failure I mode mainly occurs when CFRP laminate is 100 mm long; the shear failure II mode mainly occurs when CFRP laminate is 200 mm long; and the delamination failure mode mainly occurs when CFRP laminate is 350 mm long. Meanwhile, the thickness and the bonding methods of CFRP also influence the final failure modes in terms of CFRP length. The measurement on ultimate load shows that an increase in the length of CFRP up to 200 mm significantly improves the bearing capacity of the reinforced beam. A comparison between a theoretical analysis and the experimental observation shows a good agreement in terms of failure modes indicating the accuracy and the validity of the experiment