Protein-based (bio)materials: a way toward high-performance graphene enzymatic biosensors

Enzymes are ideal receptors for biosensors since they offer excellent selectivity and high catalytic activity. However, once removed from their native environment, enzymes present a short lifespan determining a huge drawback for their application in bio-analytical systems. The use of appropriate immobilization matrices is an effective strategy to preserve enzymatic activity. In this work, an enzymatic amperometric biosensor is designed by entrapping lactate oxidase into a protein-based immobilization matrix, formed by the self-assembly of engineered repeat proteins. Electrochemically exfoliated graphene, functionalized with cobalt phthalocyanine, is employed as electroactive material and transducer of the sensor. Due to the extraordinary enzymatic stabilization provided by the engineered protein film, the device sensitivity is preserved for more than 6 months at room temperature. Furthermore, the presented biosensor can detect lactate with outstanding performance in terms of sensitivity, repeatability, and reproducibility

Fully Conjugated Phthalocyanine Copper Metal-Organic Frameworks for Sodium-Iodine Batteries with Long-Time-Cycling Durability

Rechargeable sodium-iodine (Na-I-2) batteries are attracting growing attention for grid-scale energy storage due to their abundant resources, low cost, environmental friendliness, high theoretical capacity (211 mAh g(-1)), and excellent electrochemical reversibility. Nevertheless, the practical application of Na-I-2 batteries is severely hindered by their poor cycle stability owing to the serious dissolution of polyiodide in the electrolyte during charge/discharge processes. Herein, the atomic modulation of metal-bis(dihydroxy) species in a fully conjugated phthalocyanine copper metal-organic framework (MOF) for suppression of polyiodide dissolution toward long-time cycling Na-I-2 batteries is demonstrated. The Fe-2[(2,3,9,10,16,17,23,24-octahydroxy phthalocyaninato)Cu] MOF composited with I-2 (Fe-2-O-8-PcCu/I-2) serves as a cathode for a Na-I-2 battery exhibiting a stable specific capacity of 150 mAh g(-1) after 3200 cycles and outperforming the state-of-the-art cathodes for Na-I-2 batteries. Operando spectroelectrochemical and electrochemical kinetics analyses together with density functional theory calculations reveal that the square planar iron-bis(dihydroxy) (Fe-O-4) species in Fe-2-O-8-PcCu are responsible for the binding of polyiodide to restrain its dissolution into electrolyte. Besides the monovalent Na-I-2 batteries in organic electrolytes, the Fe-2-O-8-PcCu/I-2 cathode also operates stably in other metal-I-2 batteries like aqueous multivalent Zn-I-2 batteries. Thus, this work offers a new strategy for designing stable cathode materials toward high-performance metal-iodine batteries

NOTRY: deniable messaging with retroactive avowal

Modern secure messaging protocols typically aim to provide deniability. Achieving this requires that convincing cryptographic transcripts can be forged without the involvement of genuine users. In this work, we observe that parties may wish to revoke deniability and avow a conversation after it has taken place. We propose a new protocol called Not-on-the-Record-Yet (NOTRY) which enables users to prove a prior conversation transcript is genuine. As a key building block we propose avowable designated verifier proofs which may be of independent interest. Our implementation incurs roughly 8× communication and computation overhead over the standard Signal protocol during regular operation. We find it is nonetheless deployable in a realistic setting as key exchanges (the source of the overhead) still complete in just over 1ms on a modern computer. The avowal protocol induces only constant computation and communication performance for the communicating parties and scales linearly in the number of messages avowed for the verifier—in the tens of milliseconds per avowal

A novel triplex real-time PCR assay for the differentiation of lumpy skin disease virus, goatpox virus, and sheeppox virus

IntroductionThree members of Capripoxvirus (CaPV) genus, including lumpy skin disease virus (LSDV), goatpox virus (GTPV), and sheeppox virus (SPPV), are mentioned as notifiable forms by World Organization for Animal Health. These viruses have negatively impacted ruminant farming industry worldwide, causing great economic losses. Although SPPV and GTPV cause more severe clinical disease in only one animal species, they can transfer between sheep and goats. Both homologous and heterologous immunization strategies are used to protect animals against CaPVs. However, development of accurate and rapid methods to distinguish these three viruses is helpful for the early detection, disease surveillance, and control of CaPV infection. Therefore, we developed a novel triplex real-time PCR (qPCR) for the differentiation of LSDV, GTPV, and SPPV.MethodsUniversal primers were designed to detect pan-CaPV sequences. Species-specific minor groove binder (MGB)-based probes were designed, which were labeled with FAM for LSDV, HEX for GTPV, and ROX for SPPV. The sensitivity, specificity, reproducibility, and ability of detecting mixed infections were evaluated for the triplex qPCR. Further, 226 clinical samples of the infection and negative controls were subjected to the triplex qPCR, and the results were verified using PCR-restriction fragment length polymorphism (PCR-RFLP) and sequencing methods for PRO30 gene.ResultsThe triplex qPCR could successfully distinguish LSDV, GTPV, and SPPV in one reaction, and the assay sensitivity was 5.41, 27.70, and 17.28 copies/μL, respectively. No cross-reactivity was observed with other viruses causing common ruminant diseases, including des petits ruminants virus, foot-and-mouth disease virus, bluetongue virus, ovine contagious pustular dermatitis virus, infectious bovine rhinotracheitis virus, and bovine viral diarrhea-mucosal disease virus. Inter-and intra-assay variabilities were < 2.5%. The results indicated that the triplex qPCR was highly specific, sensitive, and reproducible. Simulation experiments revealed that this assay could successfully distinguish two or three viruses in case of mixed infections without any cross-reaction. For clinical samples, the results were completely consistent with the results of PCR-RFLP and sequencing. This demonstrated that the assay was reliable for clinical application.DiscussionThe triplex qPCR is a robust, rapid, and simple tool for identifying various types of CaPV as it can successfully distinguish LSDV, GTPV, and SPPV in one reaction. Furthermore, the assay can facilitate more accurate disease diagnosis and surveillance for better control of CaPV infection

RETRACTED ARTICLE: Extremal surface with the light-like line in Minkowski space R1+(1+1) R1+(1+1)R^{1+(1+1)}

Abstract In this paper, firstly we will give the global construction of the mixed type extremal surface in Minkowski space along the analytic light-like line. Furthermore, we construct simply the local existence of extremal surface along a single light-like line

Retraction Note to: Extremal surface with the light-like line in Minkowski space R1+(1+1) R1+(1+1)R^{1+(1+1)}

The editors have retracted this article [1] as it contains sections that substantially overlap with the following articles [2–4]. The authors have not responded to any correspondence regarding this retraction

Experiments on Turbulence Intensity and Bubble Frequency in Self-Aerated Open Channel Flows

Although spillways have been investigated experimentally by various researchers, only a few studies have been conducted on the comprehensive properties of a self-aerated air-water flow. In this study, new experimental data were recorded and discussed for the distribution of the air concentration, air-water velocity, turbulence intensity and bubble frequency in the completely developed regions for spillways. It was observed that both the turbulence intensity and bubble frequency increased from the bottom and subsequently decreased near the free surface. The positions of maximum air bubble frequency and turbulence intensity gradually approached air concentration to 0.50 in the self-aerated developed region. Self-similar relationships between the turbulence intensity and bubble frequency were proposed

Development of Polyhydroxyalkanoate-Based Polyurethane with Water-Thermal Response Shape-Memory Behavior as New 3D Elastomers Scaffolds

In this study, we report the synthesis of a novel bio-based material from polyhydroxyalkanoate (PHA) with good shape-memory effect (SME) and rapid recovery. In this PHA-based polyurethane (PHP), telechelic-hydroxylated polyhydroxyalkanoate (PHA-diols) and polyethylene glycol (PEG) were used as soft segments, providing thermo-responsive domains and water-responsive regions, respectively. Thus, PHP possesses good thermal-responsive SME, such as high shape fixing (>99%) and shape recovery ratio (>90%). Upon immersing in water, the storage modulus of PHP decreased considerably owing to disruption of hydrogen bonds in the PHP matrix. Their water-responsive SME is also suitable for rapid shape recovery (less than 10 s). Furthermore, these outstanding properties can trigger shape-morphing, enabling self-folding and self-expansion of shapes into three-dimensional (3D) scaffolds for potential biomedical applications