Hf-Nd Isotopic Variability in Mineral Dust from Chinese and Mongolian Deserts: Implications for Sources and Dispersal

Mineral dust provenances are closely related to the orogenic processes which may have distinct Hf-Nd isotopic signatures. Here we report the clay-sized (\u3c2 μm) Hf-Nd isotope data from Asian dust sources to better constrain the source and transport dynamics of dust deposition in the North Pacific. Our results show that there is a more positive radiogenic Hf isotopic composition with clay-sized fractions than the corresponding bulk sample and a decoupling of the Hf-Nd couplets in the clay formation during the weathering process. The clay-sized Hf-Nd isotopic compositions of the desert samples from the Sino-Korean-Tarim Craton (SKTC) are different from those of the Gobi and deserts from the Central Asian Orogeny Belt (CAOB) due to varying tectonic and weathering controls. The Hf-Nd isotopic compositions of dust in the North Pacific central province (NPC) match closely with those from the Taklimakan, Badain Jaran and adjacent Tengger deserts, implying that the NPC dust was mainly transported from these potential sources by the westerly jet. Our study indicates that dusts from the CAOB Gobi deserts either didn\u27t arrive in NPC or were quantitatively insignificant, but they were likely transported to the North Pacific margin province (NPM) by East Asian winter monsoon

Transcriptomic Insights Into Root Development and Overwintering Transcriptional Memory of Brassica rapa L. Grown in the Field

As the only overwintering oil crop in the north area of China, living through winter is the primary feature of winter rapeseed. Roots are the only survival organ during prolonged cold exposure during winter to guarantee flowering in spring. However, little is known about its root development and overwintering memory mechanism. In this study, root collar tissues (including the shoot apical meristem) of three winter rapeseed varieties with different cold resistance, i.e., Longyou-7 (strong cold tolerance), Tianyou-4 (middle cold tolerance), and Lenox (cold-sensitive), were sampled in the pre-winter period (S1), overwintering periods (S2–S5), and re-greening stage (S6), and were used to identify the root development and overwintering memory mechanisms and seek candidate overwintering memory genes by measuring root collar diameter and RNA sequencing. The results showed that the S1–S2 stages were the significant developmental stages of the roots as root collar diameter increased slowly in the S3–S5 stages, and the roots developed fast in the strong cold resistance variety than in the weak cold resistance variety. Subsequently, the RNA-seq analysis revealed that a total of 37,905, 45,102, and 39,276 differentially expressed genes (DEGs), compared to the S1 stage, were identified in Longyou-7, Tianyou-4, and Lenox, respectively. The function enrichment analysis showed that most of the DEGs are significantly involved in phenylpropanoid biosynthesis, plant hormone signal transduction, MAPK signaling pathway, starch and sucrose metabolism, photosynthesis, amino sugar and nucleotide sugar metabolism, and spliceosome, ribosome, proteasome, and protein processing in endoplasmic reticulum pathways. Furthermore, the phenylpropanoid biosynthesis and plant hormone signal transduction pathways were related to the difference in root development of the three varieties, DEGs involved in photosynthesis and carbohydrate metabolism processes may participate in overwintering memory of Longyou-7 and Tianyou-4, and the spliceosome pathway may contribute to the super winter resistance of Longyou-7. The transcription factor enrichment analysis showed that the WRKY family made up the majority in different stages and may play an important regulatory role in root development and overwintering memory. These results provide a comprehensive insight into winter rapeseed's complex overwintering memory mechanisms. The identified candidate overwintering memory genes may also serve as important genetic resources for breeding to further improve the cold resistance of winter rapeseed

Fund for Shared Insight: Media Analysis

Fund for Shared Insight ("Shared Insight") is a collaborative effort among fundersthat pools financial and other resources to make grants to improve philanthropy. Shared Insight believes philanthropy can have a greater social and environmental impact if foundations and nonprofits listen to the people they seek to help, act on what they hear, and openly share what they learn.Related to feedback loops, Shared Insight's work is focused on increasingthe extent to which foundations listen to others—especially the people they seek to help—and respond to their expressed interests. When Shared Insight talks about "the people they seek to help," they are referring to the individuals who receive programs and services from nonprofit organizations; for example, the students served by charter schools, the recently released prisoners benefiting from job-training services, and the low-income first-time mothers participating in prenatal through birth programs.Over the next three years, Shared Insight would hope to see changes in the amount and kind of discourse in the field related tobeneficiary feedback loops. In the summer of 2015, one year since the launch of the collaborative, ORS Impact repeated a media analysis of relevant blogs, periodicals, and reports. The following memo outlines changes in the amount and kind of discourse in the field around feedback loops compared to the year before Shared Insight launched. We raise a few observations and considerations. More detailed methodological notes follow

A hypothesis on chemical mechanism of the effect of hydrogen

<p>Abstract</p> <p>Many studies have shown that hydrogen can play important roles on the antioxidant, anti-inflammatory and other protective effects. Ohsawa et al have proved that hydrogen can electively and directly scavenge hydroxyl radical. But this mechanism cannot explain more new experimental results. In this article, the hypothesis, which is inspired by H₂ could bind to the metal as a ligand, come up to explain its extensive biology effect: Hydrogen could regulate particular metalloproteins by bonding (M–H₂ interaction) it. And then it could affect the metabolization of ROS and signal transduction. Metalloproteins may be ones of the target molecules of H₂ action. Metal ions may be appropriate role sites for H₂ molecules. The hypothesis pointed out a new direction to clarify its mechanisms.</p

Study on the relationship between multi-stage strike-slip mechanism and basin evolution in Fangzheng fault depression

Fangzheng fault depression is controlled by the northern of the Tan-Lu fault zone. It undergoes multi-stage strike-slip, extrusion modification, and erosion of the thermal uplift, forming a tectonic pattern of uplifts connected with sags. Through the regional dynamic analysis, the study of the activity law of the western Pacific plate has clarified the formation and transformation of the regional tectonic stress field. Under the background of the multi-stage of the strike-slip mechanism in the northern part of the Tan-lu fault, the Fangzheng fault depression has a characteristic of the “left-lateral strike-slip pull-apart basin, right-lateral strike-slip extrusion transformation.” According to the difference of the strike-slip, the Fangzheng fault depression has divided into two parts: the East fault depression and the West fault depression. The seismic data, seismic attribute analysis, and geological modeling techniques have applied to analyze the two fault depressions, the East fault depression has actively controlled by the strike-slip activity, and the structure is complex. The seismic data quality is poor; the structure of the West Fault Depression is the opposite and structural characteristics of asymmetrical difference strike-slip in the East and West fault depressions. Interpretation of seismic sections through a slippery background, the strike-slip attributes of the whole fault depression from south to north are segmented, and the strike-slip mechanism from east to west is different. Under the control of the multi-stage strike-slip mechanism, the Fangzheng fault depression is divided into six stages of strike-slip evolution, corresponding to the six different stages of the strike-slip control basin, the formation process of the asymmetric difference strike-slip fault basin is clarified, which provides a reference for the study of the strike-slip pull-apart basin with multi-stage structure

Clay-sized Hf-Nd-Sr isotopic composition of Mongolian dust as a fingerprint for regional to hemispherical transport

Hf-Nd-Sr isotopic fingerprinting has been employed as a powerful method for distinguishing dust sources. However, the Hf and Sr isotopic compositions of dust are highly dependent on particle size to accurately identify dust provenance. Here we compare the Hf-Nd-Sr isotopic compositions of clay-sized fractions from dust sources (the Mongolian Plateau) to sinks (Beijing and Greenland). Our results document that clay-sized Hf-Nd-Sr isotopic compositions of Mongolian dust are controlled by two isotopic/geochemical provinces: Southern Mongolian Gobi (SMG) and Northern Mongolian Plateau (NMP). Our data indicate that the SMG is potentially an important dust source to the loess in Beijing and Hulun Buir and could be a contributing source of dust to Greenland, whereas the NMP contributes little dust to the Chinese loess and the Greenland. Furthermore, we demonstrate that the deserts of northwest China are one of the main dust contributors to Beijing and Greenland, but not to Hulun Buir.</p

iTRAQ-Based Comparative Proteomic Analysis of the Roots of TWO Winter Turnip Rapes (<i>Brassica rapa</i> L.) with Different Freezing-Tolerance

The freezing tolerance of roots is crucial for winter turnip rape (Brassica rapa L.) survival in the winter in Northwest China. Cold acclimation (CA) can alleviate the root damage caused by freezing stress. To acknowledge the molecular mechanisms of freezing tolerance in winter turnip rape, two Brassica rapa genotypes, freezing stressed after the induction of cold acclimation, were used to compare the proteomic profiles of roots by isobaric tags for relative and absolute quantification (iTRAQ). Under freezing stress (&#8722;4 &#176;C) for 8 h, 139 and 96 differentially abundant proteins (DAPs) were identified in the roots of &#8220;Longyou7&#8222; (freezing-tolerant) and &#8220;Tianyou4&#8222; (freezing-sensitive), respectively. Among these DAPs, 91 and 48 proteins were up- and down-accumulated in &#8220;Longyou7&#8222;, respectively, and 46 and 50 proteins were up- and down-accumulated in &#8220;Tianyou4&#8222;, respectively. Under freezing stress, 174 DAPs of two varieties were identified, including 9 proteins related to ribosome, 19 DAPs related to the biosynthesis of secondary metabolites (e.g., phenylpropanoid and the lignin pathway), and 22 down-accumulated DAPs enriched in oxidative phosphorylation, the pentose phosphate pathway, fructose and mannose metabolism, alpha-linolenic acid metabolism, carbon fixation in photosynthetic organisms, ascorbate and aldarate metabolism. The expressional pattern of the genes encoding the 15 significant DAPs were consistent with the iTRAQ data. This work indicates that protein biosynthesis, lignin synthesis, the reduction of energy consumption and a higher linolenic acid content contribute to the freezing tolerance of winter turnip rape. Functional analyses of these DAPs would be helpful in dissecting the molecular mechanisms of the stress responses in B. rapa

iTRAQ-Based Quantitative Proteome Revealed Metabolic Changes in Winter Turnip Rape (<i>Brassica rapa</i> L.) under Cold Stress

Winter turnip rape (Brassica rapa L.) is a large-scale winter-only oil crop cultivated in Northwest China. However, its cold-resistant molecular mechanism remains inadequate. Studying the cold adaptation mechanisms of winter turnip rape based on the proteomic technique of isobaric tags for relative and absolute quantification (iTRAQ) offers a solution to this problem. Under cold stress (&#8722;4 &#176;C for eight hours), 51 and 94 differently accumulated proteins (DAPs) in Longyou 7 (cold-tolerant) and Tianyou 4 (cold-sensitive) were identified, respectively. These DAPs were classified into 38 gene ontology (GO) term categories, such as metabolic process, cellular process, catalytic activity, and binding. The 142 DAPs identified between the two cold-stressed cultivars were classified into 40 GO terms, including cellular process, metabolic process, cell, catalytic activity, and binding. Kyoto Encyclopedia of Genes and Genomes enrichment analysis indicated that the DAPs participated in 10 pathways. The abundance of most protein functions in ribosomes, carbon metabolism, photosynthesis, and energy metabolism including the citrate cycle, pentose phosphate pathway, and glyoxylate and dicarboxylate metabolism decreased, and the proteins that participate in photosynthesis&#8315;antenna and isoflavonoid biosynthesis increased in cold-stressed Longyou 7 compared with those in cold-stressed Tianyou 4. The expression pattern of genes encoding the 10 significant DAPs was consistent with the iTRAQ data. This study provides new information on the proteomic differences between the leaves of Longyou 7 and Tianyou 4 plants and explains the possible molecular mechanisms of cold-stress adaptation in B. rapa

Genome-Wide Identification of <i>GSTs</i> Gene Family and Functional Analysis of <i>BraGSTF2</i> of Winter Rapeseed (<i>Brassica rapa</i> L.) under Cold Stress

The largest gene families in plants were found to be Glutathione transferases (GSTs), which played significant roles in regulating plant growth, development, and stress response. Within the GSTs gene family, members were found to play a crucial role in the low-temperature response process of plants. A comprehensive study identified a total of 70 BraGSTs genes. Cluster analysis results demonstrated that the BraGSTs in Brassica rapa (B. rapa) could be categorized into eight sub-families and were unevenly distributed across ten chromosomes. The 39 BraGSTs genes were found to be organized into 15 tandem gene clusters, with the promoters containing multiple cis-elements associated with low-temperature response. Cold stress was observed to stimulate the expression of 15 genes, with the BraGSTF2 gene exhibiting the highest level of expression, suggesting its significant involvement in winter B. rapa’s response to low-temperature stress. Subcellular localization analysis of the BraGSTF2 protein indicated its potential expression in both the cell membrane and nucleus. The analysis of stress resistance in BraGSTF2 transgenic Arabidopsis thaliana lines demonstrated that the over-expression of this gene resulted in significantly elevated levels of SOD, POD activity, and SP content compared to the wild type following exposure to low temperatures. These levels reached their peak after 24 h of treatment. Conversely, the MDA content was lower in the transgenic plants compared to the wild-type (WT) Arabidopsis (Arabidopsis thaliana L.). Additionally, the survival rate of BraGSTF2 transgenic Arabidopsis was higher than that of the WT Arabidopsis thaliana, suggesting that the BraGSTF2 gene may play a crucial role in enhancing the cold stress tolerance of winter B. rapa. This study lays a foundation for further research on the role of the BraGSTs gene in the molecular regulation of cold resistance in winter B. rapa