Block of Tetrodotoxin-resistant Na+ Channel Pore by Multivalent Cations: Gating Modification and Na+ Flow Dependence

Tetrodotoxin-resistant (TTX-R) Na+ channels are much less susceptible to external TTX but more susceptible to external Cd2+ block than tetrodotoxin-sensitive (TTX-S) Na+ channels. Both TTX and Cd2+ seem to block the channel near the “DEKA” ring, which is probably part of a multi-ion single-file region adjacent to the external pore mouth and is involved in the selectivity filter of the channel. In this study we demonstrate that other multivalent transitional metal ions such as La3+, Zn2+, Ni2+, Co2+, and Mn2+ also block the TTX-R channels in dorsal root ganglion neurons. Just like Cd2+, the blocking effect has little intrinsic voltage dependence, but is profoundly influenced by Na+ flow. The apparent dissociation constants of the blocking ions are always significantly smaller in inward Na+ currents than those in outward Na+ current, signaling exit of the blocker along with the Na+ flow and a high internal energy barrier for “permeation” of these multivalent blocking ions through the pore. Most interestingly, the activation and especially the inactivation kinetics are slowed by the blocking ions. Moreover, the gating changes induced by the same concentration of a blocking ion are evidently different in different directions of Na+ current flow, but can always be correlated with the extent of pore block. Further quantitative analyses indicate that the apparent slowing of channel activation is chiefly ascribable to Na+ flow–dependent unblocking of the bound La3+ from the open Na+ channel, whereas channel inactivation cannot happen with any discernible speed in the La3+-blocked channel. Thus, the selectivity filter of Na+ channel is probably contiguous to a single-file multi-ion region at the external pore mouth, a region itself being nonselective in terms of significant binding of different multivalent cations. This region is “open” to the external solution even if the channel is “closed” (“deactivated”), but undergoes imperative conformational changes during the gating (especially the inactivation) process of the channel

A Comprehensive Ubiquitous Healthcare Solution on an Android™ Mobile Device

Provision of ubiquitous healthcare solutions which provide healthcare services at anytime anywhere has become more favorable nowadays due to the emphasis on healthcare awareness and also the growth of mobile wireless technologies. Following this approach, an Android™ smart phone device is proposed as a mobile monitoring terminal to observe and analyze ECG (electrocardiography) waveforms from wearable ECG devices in real time under the coverage of a wireless sensor network (WSN). The exploitation of WSN in healthcare is able to substitute the complicated wired technology, moving healthcare away from a fixed location setting. As an extension to the monitoring scheme, medicine care is taken into consideration by utilizing the mobile phone as a barcode decoder, to verify and assist out-patients in the medication administration process, providing a better and more comprehensive healthcare service

Pressure Dependence of Fragile-to-Strong Transition and a Possible Second Critical Point in Supercooled Confined Water

By confining water in nano-pores of silica glass, we can bypass the crystallization and study the pressure effect on the dynamical behavior in deeply supercooled state using neutron scattering. We observe a clear evidence of a cusp-like fragile-to-strong (F-S) dynamic transition. Here we show that the transition temperature decreases steadily with an increasing pressure, until it intersects the homogenous nucleation temperature line of bulk water at a pressure of 1600 bar. Above this pressure, it is no longer possible to discern the characteristic feature of the F-S transition. Identification of this end point with the possible second critical point is discussed.Comment: 4 pages, 3 figure

Correlation of the composite equilibrium score of computerized dynamic posturography and clinical balance tests

AbstractBackgroundThe computerized dynamic posturography has been widely used to access balance control in patients with balance dysfunction. A composite-equilibrium score (CS) can be calculated from the sensory organization test using the computerized dynamic posturography. However, the correlation between the composite equilibrium score and clinical tests and its ability to predict falls has rarely been explored in the past.MethodsA total of 60 patients with chief complaint of dizziness were enrolled in our study, and clinical assessments were done including the sensory organization test (SOT), Timed Up and Go test (TUG), Tinetti Performance-Oriented Mobility Assessment (POMA), and the dynamic gait index (DGI). The age and the subjective feeling of the severity of dizziness quantified by the visual analog scale (VAS) of each patient were also recorded.ResultsStatistical analysis revealed significant correlation between the composite equilibrium score and the TUG, POMA (gait, balance and total scores), and the DGI. However, there is statistically significant correlation between neither the CS and the age nor the VAS of dizziness. When grouping the DGI, POMA (total score), and the TUG cutoff to predict fall risks, the correlations to the CS can still be established except the TUG.ConclusionFrom the results of our study, the validity of the clinical tests was established in assessment of balance function, and clinicians can utilize these tools for preliminary evaluation of patient balance when computerized dynamic posturography is not available. In addition, CS can be used to predict the risk of falls

Distributed Enhanced Inter-Cell Interference Coordination (eICIC) in LTE-Advanced HetNets: A Potential Game Approach

International audienceIn this paper we propose a distributed algorithm for jointly optimizing almost blank subframe (ABS) and cell selection bias (CSB) patterns in Long Term Evolution-Advanced (LTE-A) heterogeneous networks (HetNets). We formulate the optimization problem as an exact potential game, where a Nash equilibrium point is guaranteed to be achieved within finite number of plays. Through simulations, we are able to demonstrate the fast convergence of the algorithm, an increase in average user rate, and a tremendous improvement on the service fairness of the users

Performance Study of Cooperative Non-Orthogonal Multiple Access with Energy Harvesting

International audienceNon-Orthogonal Multiple Access (NOMA) is a candidate channel access technique for the future generation wireless communication systems. It exploits the power domain to enable simultaneous access for multiple users. In this paper, a NOMA system with a base station (BS) and two users is studied. A novel scheme where the near user (UE 1) harvests energy from the signals sent from BS and relays the previously decoded message of the far user (UE 2) is proposed. It includes two common energy harvesting schemes, namely, power splitting and time switching, as special cases. The performance is evaluated by the achievable data rate of UE 2 under different requirements on the data rate of UE 1. Numerical simulations and analysis reveal that when the channel conditions between BS and UE 1, and between UE 1 and UE 2 are good, while that between BS and UE 2 is bad, the proposed scheme works the best and has a significant gain over the conventional NOMA scheme without cooperation. In addition, for the sake of simplicity, the power splitting scheme can be used to replace the generalized scheme for energy extraction without jeopardizing the performance gain much

Joint Optimization on Inter-cell Interference Management and User Attachment in LTE-A HetNets

International audienceTo optimize the network utility in 3GPP Long Term Evolution-Advanced (LTE-A) heterogeneous networks (HetNets), it is necessary to jointly consider inter-cell interference mitigation and user attachment. Based on potential game formulation, we optimize almost blank subframe (ABS) and/or cell selection bias (CSB) settings for both macrocells and picocells in a distributed manner. We demonstrate the need of joint ABS and CSB optimization via simulation case studies. Extensive simulations confirm that joint ABS and CSB optimizations can lead to a 20% improvement in spectral efficiency and a 46% improvement in energy efficiency while increasing the fairness of the achieved rates of users

A multi-functional PEGylated gold(iii) compound: potent anti-cancer properties and self-assembly into nanostructures for drug co-delivery

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