Parental Perspectives of Adolescent Sleep Hygiene In Suburban High-Achieving Districts

The purpose of this phenomenological qualitative study is to examine parental perceptions of adolescent sleep hygiene in Long Island High School District; a suburban mid-sized district of 6, 515 students in a predominantly high socio-economic community. Sleep hygiene is defined as the regular, healthy habits and practices in order to obtain restorative, healthy sleep. Participants of the study included parents of different ethnic backgrounds, socio-economic backgrounds, gender, and marital status from both Richmond High School East and Richmond High School West. A student’s well-being relies on purposeful, healthy sleep. Obtaining and promoting healthy sleep is both a challenge and a priority for educators as wellness is the most important foundation on which to build a successful school experience. Lack of healthy sleep has severe consequences on development and has been shown to increase risks of psychiatric diseases. The Perfect Storm Theory has become relevant in the field of student sleep and it claims that loss of sleep throughout adolescence arises from a convergence of biological, psychological, and socio-cultural influences (Carskadon, 2014). Parental perceptions of their own adolescent’s sleep hygiene is an important aspect of wellness in their child. Sleep is an important part of memory formation; therefore, insufficient sleep can jeopardizing learning and performance. Understanding these perceptions will uncover the extent of parental knowledge of their own child’s sleep hygiene and the obstacles or aids to their child’s healthy sleep

Tracing nutrient inputs into a eutrophic lake using nitrogen isotopes, Wilgreen Lake, Madison County, Kentucky

Wilgreen Lake is a dammed lake that has been classified as nutrient-impaired (303d list) by the EPA and State of Kentucky. The lake is moderately-sized covering 169 acres (0.7 km2), and drains a watershed with residential developments, cattle pasture, modified woodlands, and some industrial/urban usage in the city of Richmond. The principal tributaries are Taylor Fork and Old Town Branch that meet to form the trunk of the lake approximately one mile in length. The upper reaches of Taylor Fork are adjacent to a densely-packed (quarter-acre lots) housing development with septic systems. Old Town Branch drains cattle pasture and residential areas. Residences within these developments, while also served by septic systems, are more sparsely distributed than residences within developments adjacent to those of Taylor Fork. An ancillary tributary flowing into Pond Cove is intermittent, and drains cattle pasture and one small housing development. Recognizing and quantifying potential nutrient sources is critical to any remediation efforts in decreasing nutrient input to the lake. We hypothesize that significant nutrient input occurs from the septic systems adjacent to the shallow lake waters of Taylor Fork. We use stable nitrogen isotopes (14N and 15N) as a tracer in characterizing organic sources of nitrogen entering lake waters, and in characterizing organic sinks of nitrogen residing in the lake system. We measure the carbon-to-nitrogen ratio, carbon isotopic composition (d13C), and nitrogen isotopic composition (d15N) of organic matter held within potential nutrient sources and sinks within the Wilgreen Lake system. Potential sources include fertilizers, bovine fecal matter, human effluent from septic systems, and “natural” organic material. Sinks include plankton, macroalgae, macrophyta, and organic matter within sediments. Our samples are being measured at press time. The fundamental assumption of the test of our hypothesis is that d15N values of nitrogen sinks should reflect that of their source. With knowledge of the nitrogen isotopic composition of nitrogen sources, we may be able to recognize gradients within the nitrogen sinks of the system. Consequently, our samples of plankton, macroalgae, macrophyta, and sedimentary organic matter are taken over the entire expanse of lake

Fecal microbe distribution and abundance used as a possible proxy for nutrient source identification in eutrophic Wilgreen Lake, Madison County, Kentucky

Wilgreen Lake is a eutrophic lake that has been listed on the EPA’s 303d list as nutrient impaired. Potential sources of this impairment are from humans, cattle manure and fertilizers. We suspect that the majority of nutrients originate from human sources. As a possible proxy for nutrient input, we test our hypothesis by examining fecal microbe distribution and abundance in the lake. We took water samples at 19 sampling locations on 4 occasions. Sampling spanned 26 June to 15 August with the last 3 sampling events occurring at roughly two-week intervals. These samples were then processed using IDEXX methods, which count total coliform and Escherichia coli colonies. For sampling localities with microbial abundance that exceeded maximum sensitivity (\u3e2419 cfu/100ml), we performed dilutions of 1:4 or 1:10. Both total coliform and E. coli levels are elevated at sites adjacent to septic tank clusters. There is a gradual decline in microbial abundance distal to these populated areas and microbe levels are low in the third lake tributary that drains only cattle pasture. This strongly suggests that the source of microbial input is from septic systems; however, we cannot eliminate the possibility that fecal microbes are introduced into the lake via inflows, because of high microbial abundance in their waters. We plan on using Reverse-Transcription Polymer Chain Reaction (RT-PCR) techniques to distinguish between human and cattle sources of Bacteroides to potentially eliminate the inflows as a microbial source

Using E. coli and Bacteroides distribution and abundance in a eutrophic lake as a tracer for nutrient inputs, Wilgreen Lake, Madison County, Kentucky

Wilgreen Lake is a eutrophic lake that has been listed on the EPA’s 303d list as nutrient impaired. Potential sources of this impairment are likely from humans, cattle manure, and fertilizers. We suspect that the majority of nutrients originate from human sources, namely from septic tank effluent emanating from key housing developments ringing the lakeshore. We test our hypothesis with conventional microbial assays (Escherichia coli) and RT-PCR techniques (Bacteroides). We took water samples at 19 sampling locations on 4 occasions, and measured the abundance of Escherichia coli using IDEXX methods. Corresponding sub-samples slated for potential PCR analysis were stored at -40oC. We chose PCR assay candidates on the basis of elevated E. coli levels, and the probability of differing source contributions. There is a systematic decline in E. coli microbial abundance distal to developments with closely-spaced septic units. This suggests that the principal source of microbial input is from septic systems; however, we cannot eliminate the possibility that fecal microbes are introduced into the lake via inflows. We used quantitative PCR analysis to measure Bacteroides abundance, and to distinguish between human and cattle sources. We measured 14 samples and found total fecal microbe concentrations in all samples targeting all Bacteroides species ranged from 45 mg/L to 142 mg/L. Unlike other studies, there was no apparent relationship between the concentration of all Bacteroides species and that of E. coli. We also attempted to quantitatively determine the proportion of Bacteroides contributions from specific sources, namely human and bovine fecal matter. Although fecal contamination was measured in all 14 samples, only 1 sample had significant amounts of human fecal contamination (21%) as measured by the human-associated Bacteroides assay. None of the samples had significant amounts bovine fecal concentration as measured by the bovine-associated Bacteroides assay. These inconclusive results suggest that either there are other unidentified sources of fecal contamination by Bacteroides and/or E. coli, or that the prevailing drought conditions skewed our results by not capturing fecal transport effects due to lack of surface and/or groundwater flow

What can the activation energy tell about the energetics at grain boundaries in polycrystalline organic films?

Charge-carrier transport at the semiconductor-gate dielectric interface in organic field-effect transistors is critically dependent on the degree of disorder in the typically semi-crystalline semiconductor layer. Charge trapping can occur at the interface as well as in the current-carrying semiconductor layer itself. A detailed and systematic understanding of the role of grain boundaries between crystallites and how to avoid their potentially detrimental effects is still an important focus of research in the organic electronics community. A typical macroscopic measurement technique to extract information about the energetics of the grain boundaries is an activation energy measurement. Here, we compare detailed experiments on the energetic properties of monolayer thin films implemented in organic field-effect transistors, having controlled numbers of grain boundaries within the channel region to kinetic Monte-Carlo simulations of charge-carrier transport to elucidate the influence of grain boundaries on the extracted activation energies. Two important findings are: 1) whereas the energy at the grain boundary does not change with the number of grain boundaries in a thin film, both the measured and simulated activation energy increases with the number of grain boundaries. 2) In simulations where both energy barriers and valleys are present at the grain boundaries there is no systematic relation between the number of grain boundaries and extracted activation energies. We conclude, that a macroscopic measurement of the activation energy can serve as general quality indicator of the thin film, but does not allow microscopic conclusions about the energy landscape of the thin film

Nutrient and fecal microbe sources for a eutrophic lake and recommended remediation steps, Wilgreen Lake, Madison County, Kentucky

Wilgreen Lake is a man-made lake, classified as nutrient-impaired (303d list) by the EPA and State of Kentucky. The lake drains a watershed with residential developments, cattle pasture, modified woodlands, and some industrial/urban usage in the city of Richmond. The principal tributaries are Taylor Fork and Old Town Branch that meet to form the trunk of the lake approximately one mile in length. The upper reaches of Taylor Fork are adjacent to a densely-packed (quarter-acre lots) housing development with septic systems, and its watershed drains some portions of southern Richmond. Old Town Branch drains cattle pasture and residential areas of moderate to large lot size. An intermittent tributary flowing into Pond Cove drains cattle pasture and one small housing development. Fecal material contributes both nutrients and microbes to Wilgreen Lake. Both cattle and human fecal material enter the lake as documented by high fecal microbes counts and DNA tracing techniques. DNA tracing methods are limited by our sampling frequency but show that bovine Bacteroides microbes dominate water samples even at loci where suspected septic effluent enters the lake. The nitrogen isotopic composition (d15N) of lake plankton and algae are broadly consistent with nitrogen input from human fecal material, but results are equivocal. We suspect that large amounts of nutrients do enter the lake through septic groundwater input, however, the strong bovine signal clearly suggests deployment of remediation methods that would limit runoff from pastures adjacent to Wilgreen Lake and within its watershed. Such methods include fencing cattle off from drainages and the lake, and planting vegetative buffers around stream and lake margins. Our data alone cannot justify elimination of septic systems by costly implementation of a sewage treatment system

Amateur and Recreational Athletes’ Motivation to Exercise, Stress, and Coping During the Corona Crisis

The COVID-19 pandemic has negatively impacted mobility worldwide. As a corollary, the health of top- and lower-level athletes alike is profoundly reliant on movement and exercise. Thus, the aim of this study is to understand impacts of the COVID-19 pandemic lockdown on athletes’ motivation to exercise and train. In detail, we aim to better understand who (i.e., demographic, sport-specific, and psychological state and trait variables) reported a change in motivation to train due to the lockdown, why they reported lower motivation (i.e., open-ended questions on problems), what they did to help themselves, what support they received from others, and what they are looking forward to after the lockdown (i.e., open questions). Questionnaire data and answers to these open-ended questions were assessed via an online questionnaire, completed by 95 amateur and recreational athletes during the first COVID-19 lockdown in Germany (April to mid-May 2020). Results show that greater numbers of female athletes are less motivated to train in comparison to male athletes (p = 0.029). No differences in motivation were found regarding type of sport (individual vs. team sport) and number of competitions during the year. Also, more motivated to train amateur and recreational athletes showed lower athletic identity than athletes who reported no change in motivation to exercise during the lockdown (p = 0.03). Additionally, differences in state emotional, perceived stress, and personality variables (i.e., orientation to happiness, volition) were found between athletes who stated that they were less motivated to train compared to athletes who reported no changes in motivation. In particular, closure of sports facilities and social distancing measures were perceived to be highly problematic. Even though athletes received emotional support, organized themselves via routines and schedules, and trained using online tools, they predominately stated that they wished that their coaches would have supported them more. Understanding the impacts of a pandemic-related lockdown on athletes’ motivation, athletes’ coping strategies, and their desired support will help better support them in future crises

Resuscitating the Physician-Patient Relationship: Emergency Department Communication in an Academic Medical Center

Study objective: We characterize communication in an urban, academic medical center emergency department (ED) with regard to the timing and nature of the medical history survey and physical examination and discharge instructions. Methods: Audiotaping and coding of 93 ED encounters (62 medical history surveys and physical examinations, 31 discharges) with a convenience sample of 24 emergency medicine residents, 8 nurses, and 93 nonemergency adult patients. Results: Patients were 68% women and 84% black, with a mean age of 45 years. Emergency medicine providers were 70% men and 80% white. Of 62 medical history surveys and physical examinations, time spent on the introduction and medical history survey and physical examination averaged 7 minutes 31 seconds (range 1 to 20 minutes). Emergency medicine residents introduced themselves in only two thirds of encounters, rarely (8%) indicating their training status. Despite physician tendency (63%) to start with an open-ended question, only 20% of patients completed their presenting complaint without interruption. Average time to interruption (usually a closed question) was 12 seconds. Discharge instructions averaged 76 seconds (range 7 to 202 seconds). Information on diagnosis, expected course of illness, self-care, use of medications, time-specified follow-up, and symptoms that should prompt return to the ED were each discussed less than 65% of the time. Only 16% of patients were asked whether they had questions, and there were no instances in which the provider confirmed patient understanding of the information. Conclusion: Academic EDs present unique challenges to effective communication. In our study, the physician-patient encounter was brief and lacking in important health information. Provision of patient-centered care in academic EDs will require more provider education and significant system support

Regulation of transcription factor activity by interconnected, post-translational modifications

Transcription factors comprise just over 7% of the human proteome and serve as gatekeepers of cellular function, integrating external signal information into gene expression programs that reconfigure cellular physiology at the most basic levels. Surface-initiated cell signaling pathways converge on transcription factors, decorating these proteins with an array of post-translational modifications (PTMs) that are often interdependent, being linked in time, space, and combinatorial function. These PTMs orchestrate every activity of a transcription factor over its entire lifespan—from subcellular localization to protein–protein interactions, sequence-specific DNA binding, transcriptional regulatory activity, and protein stability—and play key roles in the epigenetic regulation of gene expression. The multitude of PTMs of transcription factors also offers numerous potential points of intervention for development of therapeutic agents to treat a wide spectrum of diseases. We review PTMs most commonly targeting transcription factors, focusing on recent reports of sequential and linked PTMs of individual factors

Biodiversité dans les cours d’eau

Des habitats diversifiés, dynamiques et proches de l’état naturel sont indispensables à la conservation et à l’amélioration de la biodiversité dans les cours d’eau. Cette fiche présente les principaux facteurs de la diversité des habitats et des espèces, ainsi que des mesures permettant d’accroître la biodiversit