MOESM2 of Infectious SIV resides in adipose tissue and induces metabolic defects in chronically infected rhesus macaques

Additional file 2. Sequence confirmation of nested PCR products, and lack of viral diversity in AT-SVF of acutely infected rhesus macaques. PCR products from SHIV Gag (A) and Env (B) 2nd round nested PCR reactions of subcutaneous and visceral AT-SVF DNA of eight infected monkeys (shown in Figure 2G) were gel-purified, sequenced, and aligned with Clustal-Omega software. Yellow-highlighted nucleotides indicate a nucleotide difference compared to other nucleotides in the alignment column (sequences include nucleotide positions A:1667 to G:2085 relative to SIVmac239 Gag, and G:7042 to C:7329 relative to HIV-1 HXB2 Env, indicated in red)