On Site

Contemporary debates on the transformation of building methods, the structure of the building industry, and the introduction of new technologies (informational, material and structural) in professional literature and in the architectural humanities tend to ignore the realities of work on construction sites. This follows a long history of failure to recognise the importance of workers’ experience and knowledge of building as a process by the key professions in the industry. The absence of the working process in accounts of historical development is exacerbated by abstract reflection on building know-how, categories of expertise, and the structure of the industry, when these are not supported by direct observation and engagement with building work and workers. Key assumptions about the relation between structural and technological changes in the industry and the knowledge, skills, composition, and requirements of the workforce can be challenged by paying attention to day-to-day activities and conditions of site work

Controlling for heterogeneity in gravity models of trade and integration

This paper compares various specifications of the gravity model of trade as nested versions of a general specification that uses bilateral country-pair fixed effects to control for heterogeneity. For each specification, we show that the atheoretical restrictions used to obtain them from the general model are not supported statistically. Because the gravity model has become the "workhorse" baseline model for estimating the effects of international integration, this has important empirical implications. In particular, we show that, unless heterogeneity is accounted for correctly, gravity models can greatly overestimate the effects of integration on the volume of trade.International trade

Controlling for heterogeneity in gravity models of trade and integration

This paper compares various specifications of the gravity model of trade as nested versions of a general specification that uses bilateral country-pair fixed effects to control for heterogeneity. For each specification, we show that the a theoretical restrictions to obtain them from the general model are not supported statistically. Because the gravity model has become the ‘workhorse’ baseline model for estimating the effects of international integration, this has important empirical implications. In particular, we show that, unless heterogeneity is accounted for correctly, gravity models can greatly overestimate the effects of integration on the volume of trade.International trade

The Parkes quarter-Jansky flat-spectrum sample 3. Space density and evolution of QSOs

We analyze the Parkes quarter-Jansky flat-spectrum sample of QSOs in terms of space density, including the redshift distribution, the radio luminosity function, and the evidence for a redshift cutoff. With regard to the luminosity function, we note the strong evolution in space density from the present day to epochs corresponding to redshifts ~ 1. We draw attention to a selection effect due to spread in spectral shape that may have misled other investigators to consider the apparent similarities in shape of luminosity functions in different redshift shells as evidence for luminosity evolution. To examine the evolution at redshifts beyond 3, we develop a model-independent method based on the V_max test using each object to predict expectation densities beyond z=3. With this we show that a diminution in space density at z > 3 is present at a significance level >4 sigma. We identify a severe bias in such determinations from using flux-density measurements at epochs significantly later than that of the finding survey. The form of the diminution is estimated, and is shown to be very similar to that found for QSOs selected in X-ray and optical wavebands. The diminution is also compared with the current estimates of star-formation evolution, with less conclusive results. In summary we suggest that the reionization epoch is little influenced by powerful flat-spectrum QSOs, and that dust obscuration does not play a major role in our view of the QSO population selected at radio, optical or X-ray wavelengths.Comment: 18 pages, 11 figures, accepted 18 Dec 2004, Astron. & Astrophys. The accepted version is expanded to include an analysis of the form of the decline in radio-QSO space density at high redshifts. This is compared with the forms of epoch dependence derived for optically-selected QSOs, for X-ray-selected QSOs, and for star formation rat

Adenosine A1 receptor activation mediates the developmental shift at layer 5 pyramidal cell synapses and is a determinant of mature synaptic strength

During the first postnatal month glutamatergic synapses between layer 5 pyramidal cells in the rodent neocortex switch from an immature state exhibiting high probability of neurotransmitter release, large unitary amplitude and synaptic depression to a mature state with decreased probability of release, smaller unitary amplitude and synaptic facilitation. Using paired recordings, we demonstrate that the developmental shift in release probability at synapses between rat somatosensory layer 5 thick-tufted pyramidal cells is due to a higher and more heterogeneous activation of presynaptic adenosine A1 receptors. Immature synapses under control conditions exhibited distributions of CV, failure rate and release probability that were almost coincident with the A1 receptor blocked condition; however, mature synapses under control conditions exhibited much broader distributions that spanned those of both the A1 receptor agonised and antagonised conditions. Immature and mature synapses expressed A1 receptors with no observable difference in functional efficacy and therefore the heterogeneous A1 receptor activation seen in the mature neocortex is due to increased adenosine concentrations that vary between synapses. Given the central role demonstrated for A1 receptor activation in determining synaptic amplitude and the statistics of transmission between mature layer 5 pyramidal cells, the emplacement of adenosine sources and sinks near the synaptic terminal could constitute a novel form of long-term synaptic plasticity

Visfatin reduces gap junction mediated cell-to-cell communication in proximal tubule-derived epithelial cells

Background/Aims: In the current study we examined if the adipocytokine, visfatin, alters connexin-mediated intercellular communication in proximal tubule-derived epithelial cells. Methods: The effects of visfatin (10-200ng/mL) on cell viability and cytotoxicity in HK2-cells were assessed by MTT, crystal violet and lactate dehydrogenase assays. Western blot analysis was used to confirm expression of Cx26, Cx40 and Cx43. The effect of visfatin (10-200ng/mL) on TGF-β1 secretion was confirmed by ELISA, and the effects of both TGF-β1 (2-10ng/mL) and visfatin (10-200ng/mL) on connexin expression were assessed by western blot. Functional intercellular communication was determined using transfer of Lucifer Yellow and paired-whole cell patch clamp electrophysiology. Results: In low glucose (5mM), visfatin (10-200ng/mL) did not affect membrane integrity, cytotoxicity or cell viability at 48hrs, but did evoke a concentration-dependent reduction in Cx26 and Cx43 expression. The expression of Cx40 was unaffected. At 48hrs, visfatin (10-200ng/mL) increased the secretion of TGF-β1 and the visfatin-evoked changes in connexin expression were mimicked by exogenous application of the pro-fibrotic cytokine (2-10ng/ml). Visfatin reduced dye transfer between coupled cells and decreased functional conductance, with levels falling by 63% as compared to control. Although input resistance was increased following visfatin treatment by 166%, the change was not significant as compared to control. The effects of visfatin on Cx-expression and cell-coupling were blocked in the presence of a TGF-β1 specific neutralizing antibody. Conclusions: The adipocytokine visfatin selectively evoked a non-toxic reduction in connexin expression in HK2-cells. The loss in gap-junction associated proteins was mirrored by a loss in functional conductance between coupled cells. Visfatin increased TGF-β secretion and the pattern of change for connexins expression was mimicked by exogenous application of TGF-β1. The effect of visfatin on Cx-expression and dye transfer were negated in the presence of a TGF-β1 neutralising antibody. These data suggest that visfatin reduces connexin-mediated intercellular communication in proximal tubule-derived epithelial cells via a TGF-β dependent pathway. © 2013 S. Karger AG, Base

"Gastroesophageal reflux disease"

Geoffrey C. Wall is an Associate Professor of Pharmacy Practice in the College of Pharmacy and Health Sciences. He can be contacted at [email protected]: Gastroesophageal reflux disease (GERD) is a pathologic condition of injury to the esophagus caused by regurgitation of gastric or gastroduodenal contents into the lumen of the esophagus. Histopathology of the esophageal mucosa may or may not be present. Gastroesophageal reflux of acid and gastric contents often causes a condition commonly referred to as heartburn. This is characterized as a retro-sternal burning sensation that radiates to the throat and interscapular region. It may be confused, even in the emergency room, with anginal pain or the onset of myocardial infarction; therefore its rapid diagnosis is important. In many patients GERD should be considered a chronic and lifelong illness and maintenance therapy is often needed. Repeated exposure of the esophagus to stomach contents leads to esophagitis. In severe cases, this can actually erode esophageal tissue (erosive esophagitis). In the last five to seven years several new treatment options for GERD have become available. These include antise-cretory agents such as the proton pump inhibitors, and new surgical techniques that have improved Nissen fundoplication safety and efficacy rates(3-4). Clinicians caring for patients with this common disorder need to understand the pathology behind GERD, its common (and uncommon) clinical manifestations, and current treatment options as recommended by the American College of Gastroenterology