Assessing the Barriers to Universal Antiretroviral Treatment Access for HIV/AIDS in South Africa

Caveolae are a type of invaginated membrane domain that has been shown to be involved in several disease states, including lipodystrophy, muscular dystrophies and cancer. Several of these diseases are caused by the lack of caveolae or caveolae-related signaling deficiencies in the tissues in which the caveolar domain are abundant such as lung, adipose, muscle and their related endothelial cells. Caveolae are formed through the assembly of the membrane inserted protein caveolin, cholesterol and the recently described family of cavin proteins, which together form the caveolae coat. The work in this thesis focuses on understanding the protein components and mechanisms that control the biogenesis and dynamics of caveolae. We have found that the protein EHD2 is an important regulator and stabilizer of the caveolar domain at the cell membrane. EHD2 is a dimeric ATPase known to oligomerize into ring-like structures around lipid membranes to control their shape. We have characterized the domain interactions involved in the specific targeting and assembly of this protein at caveolae. We propose a stringent regulatory mechanism for the assembly of EHD2 involving ATP binding and switching of the EH domain position to release the N-terminus and facilitate oligomerization in the presence of membrane species. We show that loss of EHD2 in cells results in hyper- dynamic caveolae and that caveolae stability at the membrane can be restored by reintroducing EHD2 into these cells. In a study of the protein cavin-3, which is known to be an integral component of the caveolar coat, we showed that this protein is targeted to caveolae via direct binding to the caveolar core protein caveolin1. Furthermore, we show that cavin-3 is enriched at deeply invaginated caveolae and regulate the duration time of caveolae at the cell surface. In combination with a biochemical and cellbiological approach, the advanced fluorescence microscopy techniques, like Fluorescence Recovery After Photobleaching (FRAP), Total Internal Reflection microscopy (TIRF), combined with correlative Atomic Force Microscopy (AFM) have allowed us to characterize distinct caveolae-associated proteins and their respective functions at caveolae

Malaga business review 2004

This report is the result of a Swan Grant to review the current status of business and business activity in the Malaga Business District and to map any changes that have occurred since the original Malaga Business Audit was conducted in 2001. This project was undertaken in two parts. The first part was a replication of the 2001 Malaga business survey so that the City could review trends and analyse if any of the implemented recommendations based on the 2001 results had any discernable effect. The results of the current survey were also used to inform the second part of the project, which was to develop some targeted business growth workshops that were specific to the needs of Malaga businesses. The aim of the workshops was to support and assist local businesses to develop growth strategies, local networks and connect together using external support agencies and resources. Only the results of the 2004 survey are reported here..

Georgia Agricultural Water Use Metering Program: Using Results To Benefit Farmers And The State

In their adoption of HB 579, the apparent legislative intent was to obtain clear and accurate information on the patterns and amounts of such use, which information is essential to proper management of water resources by the state and useful to farmers for improving efficiency and effectiveness of their use of water. As a part of their charge to implement this program of measuring agricultural water use, GSWCC is required to read metering devices annually, and to compile and report findings.This paper suggests approaches that might be used by the GSWCC in responding to these legislative mandates. Using data drawn from meters installed during the meter installation program's first year -- 2004 -- examples are given for types of summary statistics that might serve the GSWCC's interests in using metering data for purposes that support their more general mission of assisting farmers in their efforts to improve the management and conservation of land and water resources. We also suggest the structure of an analytical model that can be used to several important purposes, most important among which are to explore primary determinants of water use in the agricultural sector, and to assess the effectiveness of public policies in improving water use efficiency. While the peculiarities of hydrological conditions in 2004, coupled with expected data problems during the meter installation program's initial year of operation, does not allow for meaningful applications of the model when 2004 data are used, we suggest that it will play its intended role for data analyses in future years as improved data become available from the metering program.Finally, we describe a program that we are in the process of developing that will carry results from the metering program directly to the farmer in ways that should be useful to him or her in efforts to optimally manage land and water resources. This program will involve making available to farmers a secure, on-line means for accessing data, and an ability to compare their individual performance (in terms of such measures as yields and water use) with average performance measures from farms with similar characteristics. Working Paper Number 2005-00

Assessing the impact of education and marriage on labor market exit decisions of women

During the late 1990s, the convergence of women's labor force participation rates to men's rates came to a halt. This paper explores the degree to which the role of education and marriage in women's labor supply decisions also changed over this time period. Specifically, this paper investigates women's decisions to exit the labor market upon the birth of a child. The results indicate that changing exit behavior among married, educated women at this period in their lives was not likely the driving force behind the aggregate changes seen in labor force participation. Rather, changes in exit rates among single women, particularly those less educated, are much more consistent with the changing pattern of aggregate female labor force participation.