Breeding CWG-R crested wheatgrass for reduced-maintenance turf

Using reduced-maintenance turfgrass as an alternative to current high-maintenance turfgrass species would conserve resources, labor, and potentially reduce pollutants in the environment. CWG-R is an experimental population of crested wheatgrass [Agropyron cristatum (L.) Gaertn.] from Iran that has shown potential as a low-maintenance turf. The objective of this research was to estimate the genetic variation for turf traits within the CWG-R population when evaluated under a reduced-maintenance regimen. Ninety CWG-R clonal lines were established in 1998 near Logan, UT, as spaced-plant plots in a RCB design with four replicates. Maintenance of 50% ET0 replacement, 97.74 kg of nitrogen ha1yr1, and mowing at 7.62 cm was approximately 40% lower than typical for high-input Kentucky bluegrass (Poa pratensis L.) turf. Critical turf traits, including spring regrowth, season-long (March–October) and mid-summer (June–July) turf quality, color, and rhizomatous spread were evaluated in 1999 and 2000. Significant genetic variation among clonal lines was evident with broad-sense heritabilities of 0.65, 0.76, 0.45, and 0.76 for spring regrowth, season-long turf quality, color, and rhizomatous spread, respectively. Several clonal lines remained green throughout the summer months and maintained acceptable turf quality and color ratings during the critical mid-summer period. The high broad-sense heritability estimates within this population indicate potential for successful improvement of critical turf traits by phenotypic selection. These results indicate that that CWG-R could be an important low-maintenance turf-type crested wheatgrass germplasm

Salinity Reduces the Forage Quality of Forage Kochia: A Halophytic Chenopodiaceae Shrub

Forage kochia (Bassia prostrata [L.] A.J. Scott) is a perennial, halophytic Chenopodiaceae shrub adapted to semiarid rangelands and steppes. It is noted for its ability to produce edible forage in saline environments, but the effect of salinity on its nutritive value has not been determined. Therefore, this study evaluated the dose-response of increasing salinity on the forage quality of forage kochia and Gardner’s saltbush (Atriplex gardneri [Moq.] D. Dietr., a chenopod forage shrub indigenous to the United States). Individual plants were evaluated in hydroponics for 28 days at 0, 150, 300, and 600 mM NaCl. Salt from accumulated ions, minerals, and forage nutritive value were determined using ground shoot samples. Analysis of forage nutritive value is problematic in plants with high salt concentrations, so neutral detergent fiber (NDF) and in vitro true digestibility (IVTD) were also predicted on an ash-corrected dry matter (DM) basis (NDFcorrected and IVTDcorrected). Forage kochia exhibited a dose-response for salt concentration, IVTDcorrected, and crude protein (CP) as salinity increased. Salt concentrations increased to 19% of DM at 600 mM NaCl, which may reduce voluntary intake by ruminants grazing forage kochia. Results indicated that uncorrected IVTD estimates were inflated as forage kochia IVTDcorrected decreased from 65% to 56% with the major change between 300 and 600 mM NaCl. Crude protein did not differ between two forage kochia cultivars but decreased from 26% to 15% between 0 and 600 mM NaCl, whereas Gardner’s saltbush CP decreased by only five percentage points as salinity increased. Nonetheless, despite the greater CP sensitivity to salinity level, forage kochia salt concentration was less and digestibility and metabolizable energy (ME) were greater than Gardner’s saltbush. Overall, salinity reduced the forage quality of forage kochia, though not as dramatically as for Gardner’s saltbush, thus supporting use of forage kochia to improve the forage base of saline rangelands

A survey of apomixis and ploidy levels among Poa L. (Poaceae) using flow cytometry

Poa is a complex genus taxonomically and genetically. As a result, relatively little information exists for this economically important genus with regards to reproductive mode and variability in chromosome number. We examined apomixis frequency and ploidy levels in 83 Poa accessions representing 33 species from the National Plant Germplasm System using flow cytometric techniques. In reproductive mode analysis, we analyzed at least three preparations of 50 seeds each from the accessions. In ploidy level analysis, at least three plants of each accession were analyzed. Sixty percent of the species had at least one apomictic or facultative apomictic accession; 40% were sexual. Thirty-three percent of the species had no sexual accessions. Autonomous apomixis was newly identified in P. bactriana and P. bulbosa in addition to it being previously reported in P. nervosa. Poa annua and P. trivialis were identified as obligate sexual, and P. nervosa as obligate apomictic. Two of four P. palustris accessions exhibited facultative apomixis. Ploidy levels among sexually reproducing Poa accessions ranged from 2n = 2x = 14 to 2n = 12x = 84, with most accessions between 2n = 3x = 21 to 2n = 8x = 56. Among apomictic accessions, ploidy levels ranged from 2n = 3x = 21 to 2n = 10x = 70, with most between 2n = 4x = 28 and 2n = 10x = 70. These results emphasize the wide variability in Poa, both among and within species

Fatty Acid Metabolism on Pasture- and Feedlot-Finished Cattle

Feeding high-grain diets to finishing beef cattle results in high proportions of saturated fatty acids (SFA) in the beef, which is considered to confer negative impacts on human health. In contrast, pasture-finished cattle produce lower proportion of SFA, greater n-3 and less n-6 polyunsaturated fatty acids (PUFA), and higher conjugated linoleic acids (CLA) compared to high grain-finished beef (Noviandi et al. 2012). Increased n-3 PUFA, especially C18:3 n-3, can reduce the risk of heart disease, hypertension, inflammation, and mammary cancer, and lower cholesterol concentration in blood (De Deckere et al. 1998; Tapiero et al. 2002). Nitrogen fertilisation can increase biomass production and nitrogen (N) concentration of tall fescue (TF; Festuca arundinacea) (Berg and Sims 2000; Teuton et al. 2007), which positively affected beef cattle performance (Berg and Sims 1995). However, the metabolism of fatty acids (FA) in pasture-finished beef steers due to N fertilization on TF has not yet been studied. Therefore, the current study was performed to test a hypothesis that due to its potential impacts on nutrient and energy utilization, N fertilization would affect FA compositions in ruminal fluid, blood serum, and adipose tissue of pasture-finished beef steers. In addition, we were interested in beneficial effects of grazing steers by comparing the FA profiles between pasture- and feedlot-finished beef steers

Pasture Management to Improve Dry Matter Intake

Agricultural producers are constantly looking for ways to maximize returns while reducing input costs. On dairy operations, a move from confinement feeding to pasture grazing offers the potential to reduce costs associated with harvest and storage of feed. In such a transition, producers sometimes report a decline in milk production and growth of livestock—both of which can strongly correlate to dry matter intake. Fortunately, dry matter intake is something that can be influenced by management practices. In this publication, we discuss the pasture management practices to improve dry matter intake

Quantitative Trait Loci (QTL) for Forage Traits in Intermediate Wheatgrass When Grown as Spaced-Plants versus Monoculture and Polyculture Swards

It has been hypothesized that the genetic control of forage traits, especially biomass, for grass plants growing as spaced-plants versus swards is different. Likewise, the genetic control of compatibility in grass–legume polyculture mixtures is assumed to be different than for forage production in a grass monoculture. However, these hypotheses are largely unvalidated, especially at the DNA level. This study used an intermediate wheatgrass mapping population to examine the effect of three competition environments (spaced-plants, polyculture, and monoculture) on classical quantitative genetic parameters and quantitative trait loci (QTL) identification for biomass, morphology, and forage nutritive value. Moderate to high heritable variation was observed for biomass, morphological traits, and nutritive value within all three environments (H ranged from 0.50 to 0.87). Genetic correlations (rG) among environments for morphology and nutritive value were predominantly high, however, were moderately-low (0.30 to 0.48) for biomass. Six biomass QTL were identified, including three on linkage groups (LG) 1, 6, and 15 that were only expressed in the monoculture environment. Moreover, three biomass QTL on LG 10, 14, and 15 exhibited significant QTL by environment interactions. This study verified that the genetic control of grass biomass in a monoculture versus a grass–legume mixture is only partially the same, with additional genes expressed in monoculture, and that biomass in widely spaced-plants versus swards is predominantly under different genetic control. These results indicate that selection for improved grass biomass will be most successful when conducted within the targeted monoculture or polyculture sward environment per se

Development and Testing of Cool-Season Grass Species, Varieties and Hybrids for Biomass Feedstock Production in Western North America

Breeding of native cool-season grasses has the potential to improve forage production and expand the range of bioenergy feedstocks throughout western North America. Basin wildrye (Leymus cinereus) and creeping wildrye (Leymus triticoides) rank among the tallest and most rhizomatous grasses of this region, respectively. The objectives of this study were to develop interspecific creeping wildrye (CWR) × basin wildrye (BWR) hybrids and evaluate their biomass yield relative to tetraploid ‘Trailhead’, octoploid ‘Magnar’ and interploidy-hybrid ‘Continental’ BWR cultivars in comparison with other perennial grasses across diverse single-harvest dryland range sites and a two-harvest irrigated production system. Two half-sib hybrid populations were produced by harvesting seed from the tetraploid self-incompatible Acc:641.T CWR genet, which was clonally propagated by rhizomes into isolated hybridization blocks with two tetraploid BWR pollen parents: Acc:636 and ‘Trailhead’. Full-sib hybrid seed was also produced from a controlled cross of tetraploid ‘Rio’ CWR and ‘Trailhead’ BWR plants. In space-planted range plots, the ‘Rio’ CWR × ‘Trailhead’ BWR and Acc:641.T CWR × Acc:636 BWR hybrids displayed high-parent heterosis with 75% and 36% yield advantages, respectively, but the Acc:641.T CWR × ‘Trailhead’ BWR hybrid yielded significantly less than its BWR high-parent in this evaluation. Half-sib CWR × BWR hybrids of Acc:636 and ‘Trailhead’ both yielded as good as or better than available BWR cultivars, with yields similar to switchgrass (Panicum virgatum), in the irrigated sward plots. These results elucidate opportunity to harness genetic variation among native grass species for the development of forage and bioenergy feedstocks in western North America

Influence of Harvest Date on Seed Yield and Quality in Forage Kochia

Forage kochia (Bassia prostrata) is used for rangeland reclamation and livestock and wildlife forage, but limited research has been conducted on its seed production. Therefore, this research evaluated the effect of harvest date on seed weight, germination, and seed yield of forage kochia subspecies virescens and grisea. Seed was harvested from individual plants for 3 years during October, November, and December. October harvest had the lightest 100-seed weights, with the November harvest slightly heavier than December, for most accessions. Cultivar Snowstorm and breeding line Sahsel, both subsp. grisea, had the greatest 100-seed weights in November, 155 and 143 mg, respectively, whereas, cv. Immigrant (subsp. virescens), the standard for forage kochia, ranked among the least for 100-seed weight. For most accessions, germination was lowest from the October harvest (11%–43%), with greater germination with November and December harvested seeds (43%–64%). Viable seed yields were greatest in November with the exception of two accessions, which peaked in October, indicating earlier maturity. Results indicate that forage kochia usually reaches optimum seed maturity by early November, after plants are exposed to freezing temperatures; however, earlier maturing accessions exist in both subspecies virescens and grisea

Characterization of Testing Locations for Developing Cool-Season Grass Species

The identification of best testing locations facilitates the allocation of resources in a breeding program, allowing emphasis to be placed at the sites best suited for identifying superior plant materials for the target environment. The objective of this study was the identification of best locations for the evaluation and testing of cool-season grass species within the Northern Great Plains and Intermountain regions of the USA. This study also sought to subdivide the locations into meaningful environmental groupings based on similar entry performance. The study characterized initial stand frequency and forage production (over a 3-yr period) of crested wheatgrass [Agropyron cristatum (L.) Gaertn.; A. desertorum (Fisch. ex Link) Schultes; A. fragile (Roth) Candargy], intermediate wheatgrass [Thinopyrum intermedium (Host) Barkworth & D.R. Dewey], and smooth bromegrass (Bromus inermis Leyss.) at six locations within these regions. Results suggested the existence of best testing locations and environmental groupings for each of the species. For example, the Ithaca, NE, location was consistently a good location for testing forage production. Although there were some consistencies, generally, the best testing locations and environmental groupings were species and trait specific. Thus, the targeted use of locations appeared to be most useful on an individual species basis, rather than considered across the cool-season grass species