A: Representative histological sections of jejunum showing the goblet cells with haematoxylin and eosin stain in mice on day 5 challenged with 5-FU (IP). They were fed with probiotics (<i>Lcr35</i> or <i>LaBi</i>) or saline. The arrows indicated goblet cells. The image acquisition phase was done with a 20x magnification objective. Scale bar = 50μm. B: Jejunal goblet cells after staining were counted. Values were represented as mean ± SEM and were analyzed using one-way ANOVA.

<p>A: Representative histological sections of jejunum showing the goblet cells with haematoxylin and eosin stain in mice on day 5 challenged with 5-FU (IP). They were fed with probiotics (<i>Lcr35</i> or <i>LaBi</i>) or saline. The arrows indicated goblet cells. The image acquisition phase was done with a 20x magnification objective. Scale bar = 50μm. B: Jejunal goblet cells after staining were counted. Values were represented as mean ± SEM and were analyzed using one-way ANOVA.</p

Diarrhea score after administrating probiotics (<i>Lcr35</i> or <i>LaBi</i>) with/without 5-FU treatment.

<p>The mice were recorded daily and the results of all groups were compared with those in 5-FU + saline group for 5 days. In the control groups, the mice injected saline and administrated with saline (○), <i>Lcr35</i> (□) and <i>LaBi</i> (△). In the experimental groups, the mice injected 5-FU and administrated with saline (●), <i>Lcr</i> (■) and <i>LaBi</i> (▲). The severity of diarrhea was attenuated in those mice treated with probiotics in the 5-FU groups. The data with different superscripted letters are significantly different based on the one-way ANOVA.</p

Up-regulations of IL-6, IL-1β and TNF-α in mucositis mice were followed after injection with 5-FU.

<p>Mucositis mice were fed with (+) or without (−) probiotics. Gene expressions of IL-6, IL-1β and TNF-α were determined by Q-PCR (A) jejunum tissue (B) colon tissue. Induction of cytokine expressions were presented as RQ compared to 18sRNA housekeeping gene expression. The data with different superscripted letters are significantly different based on the one-way ANOVA.</p

A: Representative histology of jejunum showing villus height and crypt depth with haematoxylin and eosin stain in mice on day 5 challenged with 5-FU (IP). They were fed with probiotics (<i>Lcr35</i> or <i>LaBi</i>) or saline. The image acquisition phase was done with a 20x magnification objective. Scale bar = 200μm. B: Values were represented as mean ± SEM and were analyzed using one-way ANOVA. Segments of jejunum were taken for measurement of villus height, crypt depth and villus/crypt ratio per mouse.

<p>A: Representative histology of jejunum showing villus height and crypt depth with haematoxylin and eosin stain in mice on day 5 challenged with 5-FU (IP). They were fed with probiotics (<i>Lcr35</i> or <i>LaBi</i>) or saline. The image acquisition phase was done with a 20x magnification objective. Scale bar = 200μm. B: Values were represented as mean ± SEM and were analyzed using one-way ANOVA. Segments of jejunum were taken for measurement of villus height, crypt depth and villus/crypt ratio per mouse.</p

Daily body weight change in percentage of saline or 5-FU-injected mice with/without probiotics (<i>Lcr35</i> or <i>LaBi</i>) administration.

<p>The mice were weighted daily and the results of all groups were compared with those in 5-FU-saline groups for 5 days. In the control groups, the mice were injected saline and administrated with saline (○), <i>Lcr35</i> (□) and <i>LaBi</i> (△). In the experimental groups, the mice were injected 5-FU and administrated with saline (●), <i>Lcr35</i> (■) and <i>LaBi</i> (▲). Data of starting bodyweight are expressed 100% from day 0.</p

Serum levels of TNF-α, IL-6 and IFN-γ by ELISA assays from mice challenged by 5-FU-induced intestinal mucositis on the 5^th day.

<p>They were fed with (+) or without (−) probiotics (<i>Lcr35/LaBi</i>). The data with different superscripted letters are significantly different based on the one-way ANOVA.</p