Profile Of Bacteria And Fungi On Money Coins

Objectives: To determine the quantity and quality of bacterial and fungi on money coins and to identify those that could pose a public health risk.Design: Random sampling of coins from subjects within predetermined categories.Setting: Westlands division of Nairobi Metropolitan province.Subjects: Twenty-shilling coin samples were collected from matatu (a common commuter vehicle in Kenya) taxi conductors, greengrocers, shoe shiners, butchers, food kiosk/restaurant attendants, grocery shops attendants, roast maize vendors and school children. Forty coin samples were analysed for both the total viable content and the types of bacterial and fungal organisms.Results: Average bacterial content on the coins ranged from 2.3xl03 to 25.5x103 and fungi content from 11 to 377 colony forming units. The following potentially pathogenic bacteria were among those isolated: Escherichia coli, Klebsiella, Serratia, Enterobacter, Salmonella,Acinetobacter, Enterococci, Staphylococcus and Bacillus cereus. In addition, this is the first report of potentially pathogenic fungal isolation from money coins. Penicillium spp, Aspergillus niger, Fusarium, Rhizopus, Altenaria spp, Candida spp and Cryptococcus were isolated. Conclusion: Money coins harbour potentially pathogenic bacteria and fungi that may pose a public health risk. Hand hygiene is therefore strongly recommended, especially for those who simultaneously handle food and money

Effect of protein supplementation and urea treatment on utilization of maize stover by Red Maasai sheep

Six Red Maasai sheep were used to investigate the effects of urea treatment and cotton seed cake supplementation of maize stover on intake, digestibility and rumen fermentation parameters. The basal feeds were Rhodes grass (Chloris gayana) hay (H), untreated maize (Zea mays) stover (US) and treated maize stover (TS), each of which was supplemented with 100 g of cotton seed cake (CSC) to make H+CSC, US+CSC and TS+CSC. These were offered over four feeding periods in a completely randomized block design, with periods serving as blocks. Hay and US treatments had similar intakes, digestibility, ruminal fluid volatile fatty acid (VFA) concentrations and pH. Urea treatment of the stover increased the digestibility of dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fibre (NDF) and cellulose as well as the rumen ammonia nitrogen (AN) concentration. Cotton seed cake supplementation increased DM, OM and CP digestibility in US from 445 to 614 g/kg, 475 to 633 g/kg and 308 to 604 g/kg, respectively and VFA concentration in the rumen from 31 to 196 mmol/L. Supplementation of treated stover, increased the AN concentration (137 vs. 234 mg/l) rumen in the rumen content with no effect on nutrient digestibility and VFA concentration. The pH of the ruminal content for the diets ranged from 6.83 to 7.12. The trends of rumen AN and VFA for supplemented diets showed a gradual rise to a maximum three hours post-feeding, followed by a decrease in their respective concentrations. It was concluded that urea treatment and CSC supplementation of stover increased its utilization but supplementation of treated stover with CSC was not effective. Key Words: Sheep, Ruminal fermentation, Maize stover, Urea treatment SA Jnl Animal Sci Vol.34(1) 2004: 23-3

In vitro anthelmintic effects of crude aqueous extracts of Tephrosia vogelii, Tephrosia villosa and Carica papaya leaves and seeds

The prevalence of anthelmintic resistance and the consumer demand for alternative farming systems that limit the use of chemical anthelmintics has made the search for alternative gastrointestinal nematode parasites control methods crucial. Traditional medicinal/herbal plants can offer an alternative to the reliance on chemical anthelmintic drugs. This study evaluates the efficacy of crude aqueous extracts of Tephrosia vogelii Hook., Tephrosia villosa Pers., and Carica papaya Linn. leaves and Carica papaya Linn. seeds against gastrointestinal nematodes using in vitro egg hatch and larval development inhibition assays. Rectal faecal samples from sheep were subjected to parasitological examination for faecal egg counts (FEC) using the McMaster counting technique. 100 g of dried and poultice aqueous leaf extract of T. vogelii, T. villosa, C. papaya leaves and seeds was blended into liquefaction in 200 ml of distilled water then boiled at 90-100°C for 1 h and cooled. Levamisol and distilled water were used as positive and negative control in the bioassay. Egg hatch assay revealed more than 95.8% reduction in egg hatch at concentration of 500 mg/ml for dried and poultice paste of T. vogelii leaves and C. papaya seeds. Larval development inhibition assay results showed that both dried and poultice paste of T. vogelii leaves and C. papaya seeds extract yielded more than 98% inhibition at a concentration of 500 mg/ml. Based on the LD50 dried extract of C. papaya seeds was most potent extracts for the inhibition of both egg hatching (49.94 mg/ml) and larval development (49.32 mg/ml). Both poultice and dried extract for all the plants showed significant and dose dependent egg and larval development inhibition. These findings indicate that the evaluated plants have potential anthelmintic effect and could provide viable alternatives for the control of gastrointestinal helminthes in ruminants.Keywords: Aqueous extracts, Anthelmintic activity, Medicinal plants, Tephrosia vogelii, Tephrosia villosa, Carica papaya.African Journal of Biotechnology, Vol 13(52) 4667-467