Nanosensors for diagnosis with optical, electric and mechanical transducers

Nanosensors with high sensitivity utilize electrical, optical, and acoustic properties to improve the detection limits of analytes. The unique and exceptional properties of nanomaterials (large surface area to volume ratio, composition, charge, reactive sites, physical structure and potential) are exploited for sensing purposes. High-sensitivity in analyte recognition is achieved by preprocessing of samples, signal amplification and by applying different transduction approaches. In this review, types of signals produced and amplified by nanosensors (based on transducers) are presented, to sense exceptionally small concentrations of analytes present in a sample. The use of such nanosensors, sensitivity and selectivity can offer different advantages in biomedical applications like earlier detection of disease, toxins or biological threats and create significant improvements in clinical as well as environmental and industrial outcomes. The emerging discipline of nanotechnology at the boundary of life sciences and chemistry offers a wide range of prospects within a number of fields like fabrication and characterization of nanomaterials, supramolecular chemistry, targeted drug supply and early detection of disease related biomarkers

Role of structural dynamics in selectivity and mechanism of non-heme Fe(II) and 2-Oxoglutarate-dependent Oxygenases involved in DNA repair

AlkB and its human homologue AlkBH2 are Fe(II)- and 2-oxoglutarate (2OG)-dependent oxygenases that repair alkylated DNA bases occurring as a consequence of reactions with mutagenic agents. We used molecular dynamics (MD) and combined quantum mechanics/molecular mechanics (QM/MM) methods to investigate how structural dynamics influences the selectivity and mechanisms of the AlkB- and AlkBH2-catalyzed demethylation of 3-methylcytosine (m3C) in single (ssDNA) and double (dsDNA) stranded DNA. Dynamics studies reveal the importance of the flexibility in both the protein and DNA components in determining the preferences of AlkB for ssDNA and of AlkBH2 for dsDNA. Correlated motions, including of a hydrophobic β-hairpin, are involved in substrate binding in AlkBH2–dsDNA. The calculations reveal that 2OG rearrangement prior to binding of dioxygen to the active site Fe is preferred over a ferryl rearrangement to form a catalytically productive Fe(IV)═O intermediate. Hydrogen atom transfer proceeds via a σ-channel in AlkBH2–dsDNA and AlkB–dsDNA; in AlkB–ssDNA, there is a competition between σ- and π-channels, implying that the nature of the complexed DNA has potential to alter molecular orbital interactions during the substrate oxidation. Our results reveal the importance of the overall protein–DNA complex in determining selectivity and how the nature of the substrate impacts the mechanism

An adaptive opposition-based learning selection: the case for Jaya algorithm

Over the years, opposition-based Learning (OBL) technique has been proven to effectively enhance the convergence of meta-heuristic algorithms. The fact that OBL is able to give alternative candidate solutions in one or more opposite directions ensures good exploration and exploitation of the search space. In the last decade, many OBL techniques have been established in the literature including the Standard-OBL, General-OBL, Quasi Reflection-OBL, Centre-OBL and Optimal-OBL. Although proven useful, much existing adoption of OBL into meta-heuristic algorithms has been based on a single technique. If the search space contains many peaks with potentially many local optima, relying on a single OBL technique may not be sufficiently effective. In fact, if the peaks are close together, relying on a single OBL technique may not be able to prevent entrapment in local optima. Addressing this issue, assembling a sequence of OBL techniques into meta-heuristic algorithm can be useful to enhance the overall search performance. Based on a simple penalized and reward mechanism, the best performing OBL is rewarded to continue its execution in the next cycle, whilst poor performing one will miss cease its current turn. This paper presents a new adaptive approach of integrating more than one OBL techniques into Jaya Algorithm, termed OBL-JA. Unlike other adoptions of OBL which use one type of OBL, OBL-JA uses several OBLs and their selections will be based on each individual performance. Experimental results using the combinatorial testing problems as case study demonstrate that OBL-JA shows very competitive results against the existing works in term of the test suite size. The results also show that OBL-JA performs better than standard Jaya Algorithm in most of the tested cases due to its ability to adapt its behaviour based on the current performance feedback of the search process

Trend of transfusion transmitted infections frequency in blood donors: provide a road map for its prevention and control

<p>Abstract</p> <p>Background</p> <p>Transfusion transmitted infections create significant burden on health care system. Donor selection is of paramount importance because infected individuals serve as an asymptomatic reservoir and a potential source of transmission.</p> <p>Methods</p> <p>A retrospective study was carried out in healthy blood donors in the Lady Reading Hospital Peshawar, Pakistan over a period of three and a half years i.e., from January 2008 to June 2011, to determine the prevalence of HBV, HCV, HIV and syphilis in order to provide information for relevant polices.</p> <p>Results</p> <p>Out of 1,27,828 sample of blood donors, recorded mean prevalence for HBs Ag, anti-HCV, anti-HIV and syphilis was 2.68%, 2.46%, 0.06% and 0.43%, respectively, with an increasing trend in frequencies of transfusion transmitted infections (TTIs).</p> <p>Conclusions</p> <p>This study reflects that blood transfusion is one of the leading risk factor of spread of the TTIs, which showed the need and importance of the mandatory screening of these infectious markers in blood donations.</p

Latin hypercube sampling Jaya algorithm based strategy for T-way test suite generation

T-way testing is a sampling strategy that generates a subset of test cases from a pool of possible tests. Many t-way testing strategies appear in the literature to-date ranging from general computational ones to meta-heuristic based. Owing to its performance, man the meta-heuristic based t-way strategies have gained significant attention recently (e.g. Particle Swarm Optimization, Genetic Algorithm, Ant Colony Algorithm, Harmony Search, Jaya Algorithm and Cuckoo Search). Jaya Algorithm (JA) is a new metaheuristic algorithm, has been used for solving different problems. However, losing the search's diversity is a common issue in the metaheuristic algorithm. In order to enhance JA's diversity, enhanced Jaya Algorithm strategy called Latin Hypercube Sampling Jaya Algorithm (LHS-JA) for Test Suite Generation is proposed. Latin Hypercube Sampling (LHS) is a sampling approach that can be used efficiently to improve search diversity. To evaluate the efficiency of LHS-JA, LHS-JA is compared against existing metaheuristic-based t-way strategies. Experimental results have shown promising results as LHS-JA can compete with existing t-way strategies

Measurement of branching fraction and direct CPCP asymmetry in charmless B+→K+K−π+B^+ \to K^+K^- \pi^+ decays at Belle

We report a study of the charmless hadronic decay of the charged $B$ meson to the three-body final state $K^+ K^- \pi^+$. The results are based on a data sample that contains $772\times10^6$ $B \bar{B}$ pairs collected at the $\Upsilon(4S)$ resonance with the Belle detector at the KEKB asymmetric-energy $e^+ e^-$ collider. The measured inclusive branching fraction and the direct $CP$ asymmetry are $(5.38\pm0.40\pm0.35)\times 10^{-6}$ and $-0.170\pm0.073\pm0.017$, respectively, where the first uncertainties are statistical and the second are systematic. The $K^{+}K^{-}$ invariant mass distribution of the signal candidates shows an excess in the region below $1.5$ GeV/$c^2$, which is consistent with the previous studies from BaBar and LHCb. In addition, strong evidence of a large direct $CP$ asymmetry is found in the $K^{+}K^{-}$ low-invariant-mass region.Comment: 7 pages, 3 figure

Search for B→hννˉ\boldsymbol{B\to h\nu\bar{\nu}} decays with semileptonic tagging at Belle

We present the results of a search for the rare decays $B\to h\nu\overline{\nu}$, where $h$ stands for $K^+,\:K^0_{\mathrm{S}},\:K^{\ast +},\:K^{\ast 0},\:\pi^+,\:\pi^0,\:\rho^+$ and $\rho^{0}$. The results are obtained with $772\times10^{6}$ $B\overline{B}$ pairs collected with the Belle detector at the KEKB $e^+ e^-$ collider. We reconstruct one $B$ meson in a semileptonic decay and require a single $h$ meson but nothing else on the signal side. We observe no significant signal and set upper limits on the branching fractions. The limits set on the $B\to K^0_{\mathrm{S}}\nu\overline{\nu}$, $B^0\to K^{*0}\nu\overline{\nu}$, $B\to \pi^+\nu\overline{\nu}$, $B^0\to\pi^0\nu\overline{\nu}$, $B^+\to\rho^+\nu\overline{\nu}$, and $B^0\to\rho^0\nu\overline{\nu}$ channels are the world's most stringent.Comment: Submitted to PR

Measurement of the branching fraction and CPCP asymmetry in B0→π0π0B^{0} \to \pi^{0}\pi^{0} decays, and an improved constraint on ϕ2\phi_{2}

We measure the branching fraction and $CP$ violation asymmetry in the decay $B^{0}\to \pi^{0}\pi^{0}$, using a data sample of $752\times 10^{6}$ $B\bar{B}$ pairs collected at the $\Upsilon(4S)$ resonance with the Belle detector at the KEKB $e^{+}e^{-}$ collider. The obtained branching fraction and direct $CP$ asymmetry are $\mathcal{B}(B\to \pi^{0}\pi^{0}) = [1.31 \pm 0.19~ \text{(stat.)} \pm 0.19~ \text{(syst.)}] \times 10^{-6}$ and $A_{CP} = +0.14 \pm 0.36~ \text{(stat.)} \pm 0.10~ \text{(syst.)},$ respectively. The signal significance, including the systematic uncertainty, is 6.4 standard deviations. We combine these results with Belle's earlier measurements of $B^{0}\to \pi^{+} \pi^{-}$ and $B^{\pm} \to \pi^{\pm} \pi^{0}$ to exclude the $CP$-violating parameter $\phi_{2}$ from the range $15.5^{\circ} < \phi_{2} < 75.0^{\circ}$ at 95\% confidence level.Comment: 8 pages, 2 figure

Search for CPCP violation in the D+→π+π0D^{+}\to\pi^{+}\pi^{0} decay at Belle

We search for $CP$ violation in the charged charm meson decay $D^{+}\to\pi^{+}\pi^{0}$, based on a data sample corresponding to an integrated luminosity of $\rm 921~fb^{-1}$ collected by the Belle experiment at the KEKB $e^{+}e^{-}$ asymmetric-energy collider. The measured $CP$ violating asymmetry is $[+2.31\pm1.24({\rm stat})\pm0.23({\rm syst})]\%$, which is consistent with the standard model prediction and has a significantly improved precision compared to previous results.Comment: 8 pages, 3 figure