ANALISIS KADAR KLORIN PADA TEH CELUP BERDASARKAN WAKTU SEDUHAN

Background: Tea is the most widely consumed beverages by all levels of society because in addition to economical, tea is also thought to provide health benefits. Compounds that contribute to the health of the body such as tannins, catechins, flavanols and cafein. Along with its development era community prefers tea bag as it is easy and practical use. Without realizing it, the longer the brewed tea bag in the water substance called chlorine bleach paper contained in tea bags rather the bag of tea bags will also be dissolved.   Method: This research includes the study of laboratory experiments. Object in this study is 4 (four) brand of tea bag and then examined in a Laboratory Ambon Pattimura University Biology Education using thiosulfate titration methode. Result: Anova and Tukey test results indicate that there are differences in the levels of chlorine-based treatment duration and type of tea steeping. The study test showed was obtained at the highest levels of chlorine treatment A1P4 (0,413 ppm) and A4P1 treatment resulted in the lowest levels of chlorine (0,058 ppm). Conclusion: There are differences in the levels of chlorine in various brands of tea bag is Sariwangi tea, sosro tea, poci tea and tong tji tea for time 2 minutes, 4 minutes, 6 minutes and 8 minutes

Induksi Polifenol Mahkota Dewa dan Apoptosis Sel Kanker Paru Mencit Strain Balb/C: Analisis pada Up-Regulation Bax dan Down-Regulation Bcl-2

Mahkota Dewa polyphenol induction and apoptosis cell lung cancer in Balb/c strain mice analysis on up-regulation bax and downregulation Bcl-2Background: This research is aimed to prove the role of Mahkota Dewa polyphenol in the up-regulation Bax and down-regulation in strain Balb/c mice which inducted with benzo(a)pyrene (BaP).Method: Post test control group design with 30 strain Balb/c mice sample, aged 1-2 weeks, weighed 20-30 grams, healthy mice condition. All mice inducted with BaP and then randomized into 2 groups, which were the control group and the treatment group (polyphenol 50mg). The development of the lung carcinogenesis was observed by tissue surgery in the 8th, 17th and 26th week. The data collected include expression examination Bax and Bcl-2. The data analysis was conducted by Kruskal-Wallis, Mann-Whitneyand correlation test of Spearman's with significance degree of p<a (0.05).Result: The oral administration of Mahkota Dewa polyphenol of 50 mg were significantly decreased the occurance of lung carcinogenesis through decreasing of protein Bax and increasing Bcl-2 in treatment group in week 8, 17 and 26 (p=0.000). Protein Bax for the control group for week 8 were 4.04±0.22 and 1.92±0.10 in week 26, while the treatment group were 5.96±0.32 and 4.68±0.22 (p=0.000). Protein Bcl-2 for the control group for week 8 were 5.80±0.32 and 9.64±0.26 in week 26, while the treatmentgroup were 5.12±0.22 and 7.38±0.21 (p=0.000). The Spearman correlation for Bax and Bcl-2 with significancy value of (p=0.000).Conclusion: The administration of Mahkota Dewa polyphenol of 50 mg effectively increased Bax protein expression and decreased Bcl-2 protein expression in mice Balb/c

Pengaruh Ekstrak Alpinia Galanga L terhadap Produksi Biofilm pada Escherichia Coli

Escherichia coli merupakan bakteri Gram negatif berbentuk batang, mampu menyebabkan infeksi di beberapa bagian tubuh, dan ditemukan telah resisten terhadap berbagai antibiotic, salah satu factor penyebabnya adalah kemampuannya membentuk biofilm pada jaringan. Alpinia gallanga L memiliki kemampuan menghambat pembentukan biofilm terhadap beberapa bakteri. Tujuan Penelitian ini adalah untuk mengetahui kemampuan ekstrak Alpinia galangal L dalam menghambat produksi biofilm Escherichia coli. Penelitian ini di awali dengan ekstraksi rimpang lengkuas dengan etanol menggunakan metode masersi, kemudian dibuat konsentrasi 10%, 20%, 30%, 40%, dan 50%. Escherichia coli diisolasi dari kasus Infeksi Saluran Kemih (ISK). kemudian di lakukan karakterisasi fisiologisnya dan uji kepekaan terhadap antibiotik. Uji penghambatan biofilm Escherichia coli dilakukan dengan metode microtiter plate culture dengan menggunakan spektrofotometer pada panjang gelombang 595nm, Hasil pengukuran produksi biofilm berupa besarnya nilai Optical Density crystal violet 0,1%, setiap perlakuan menggunakan ulangan 8 kali, data yang didapatkan dianalisis dengan One Way Anova. Hasil Penelitian menunjukan bahwa Ekstrak Alpinia galanga L mampu menghambat produksi biofilm Escherichia coli pada konsentrasi 30%