COMETABOLIC DEGRADATION OF CHLOROALLYL ALCOHOLS IN BATCH AND CONTINUOUS CULTURES

The biodegradation of chloroallyl alcohols by pure and mixed bacterial cultures was investigated. Only 2-chloroallyl alcohol and cis- and trans-3-chloroallyl alcohol served as growth substrate for pure cultures. The other chloroallyl alcohols could be cometabolically degraded during growth on 2-chloroallyl alcohol. Cometabolic degradation of trichloroallyl alcohol, which was the most recalcitrant congener, by a Pseudomonas strain isolated on 2-chloroallyl alcohol resulted in 60% dechlorination. Efficient degradation of a mixture of chloroallyl alcohols in continuous culture could only be achieved in the presence of a satellite population. The mixed culture degraded 99% of the total chloroallyl alcohols added with 71% chloride release. The culture contained strains with a new catabolic potential. The results indicate the importance of mixed cultures and genetic adaptation for efficient chloroallyl alcohol removal

Omgaan met bodemverontreiniging in het landelijk gebied

Bodemverontreiniging kan leiden tot ecologische effecten en risico's voor plant en dier. Soms moeten er daarom maatregelen worden getroffen. In de praktijk van natuurbeheer en natuurontwikkeling is er nog veel onduidelijkheid over hoe deze effecten bepaald moeten worden. Daartoe is door VROM, LNV, RIZA, RIVM en Bioclear (mede gefinancierd door SKB) PERISCOOP opgezet (Platform Ecologische Risicobeoordeling

Cometabolic degradation of chloroallyl alcohols in batch and continuous cultures

The biodegradation of chloroallyl alcohols by pure and mixed bacterial cultures was investigated. Only 2-chloroallyl alcohol and cis- and trans-3-chloroallyl alcohol served as growth substrate for pure cultures. The other chloroallyl alcohols could be cometabolically degraded during growth on 2-chloroallyl alcohol. Cometabolic degradation of trichloroallyl alcohol, which was the most recalcitrant congener, by a Pseudomonas strain isolated on 2-chloroallyl alcohol resulted in 60% dechlorination. Efficient degradation of a mixture of chloroallyl alcohols in continuous culture could only be achieved in the presence of a satellite population. The mixed culture degraded 99% of the total chloroallyl alcohols added with 71% chloride release. The culture contained strains with a new catabolic potential. The results indicate the importance of mixed cultures and genetic adaptation for efficient chloroallyl alcohol removal.

Monitoren in-situ saneringsprocessen Hollandsche IJssel

The options for on-site decontamination have been inventoried on the basis of various studies carried out on a natural bank of the Hollandsche IJssel. In addition to standard studies of the beds of water courses, this research consisted of recording flora and fauna, an exhaustive risk analysis, availability research relating to PAC and heavy metals and determination of the potential for natural degradation. The research revealed that reconstruction of the site in combination with on-site decontamination is an option that will allow the preservation of the existing flora, fauna and landscape. On-site decontamination measures may consist of stimulating biological degradation, promoting sedimentation processes in such a way that the contaminated layers of sediment are covered (natural capping) or the partial removal of the existing sludge and its replacement with clean sedimen