8 research outputs found
Histopatologia de fígado, rim e baço de piaractus mesopotamicus, prochilodus lineatus e pseudoplatystoma fasciatum parasitados por myxosporídios, capturados no Rio Aquidauana, Mato Grosso do Sul, Brasil
Residual stress evaluation within hydroxyapatite coatings of different micrometer thicknesses
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Imaging of cardiopulmonary diseases
Calcium phosphate (CaP) ceramic coatings have been used to enhance the biocompatibility and osteoconductive properties of metallic implants. The chemical composition of these ceramic coatings is an important parameter, which can influence the final bone performance of the implant. In this study, the effect of phase composition of CaP-sputtered coatings was investigated on in vitro dissolution behavior and in vivo bone response. Coatings were prepared by a radio frequency (RF) magnetron sputtering technique; three types of CaP target materials were used to obtain coatings with different stoichiometry and calcium to phosphate ratios (hydroxyapatite (HA), alpha-tricalciumphosphate (alpha-TCP), and tetracalciumphosphate (TTCP)) were compared with non-coated titanium controls. The applied ceramic coatings were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and inductively coupled plasma optical emission spectroscopy. The in vitro dissolution/precipitation of the CaP coatings was evaluated using immersion tests in simulated body fluid (SBF). To mimic the in vivo situation, identical CaP coatings were also evaluated in a femoral condyle rabbit model. TCPH and TTCPH showed morphological changes during 4-week immersion in SBF. The results of bone implant contact (BIC) and peri-implant bone volume (BV) showed a similar response for all experimental coatings. An apparent increase in tartrate resistant acid phosphatase (TRAP) positive staining was observed in the peri-implant region with decreasing coating stability. In conclusion, the experimental groups showed different coating properties when tested in vitro and an apparent increase in bone remodeling with increasing coating dissolution in vivo
Substrate geometry directs the in vitro mineralization of calcium phosphate ceramics
Repetitive concavities on the surface of bone implants have recently been demonstrated to foster bone formation when implanted at ectopic locations in vivo. The current study aimed to evaluate the effect of surface concavities on the surface mineralization of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) ceramics in vitro. Hemi-spherical concavities with different diameters were prepared at the surface of HA and β-TCP sintered disks: 1.8 mm (large concavity), 0.8 mm (medium concavity) and 0.4 mm (small concavity). HA and β-TCP disks were sintered at 1100°C or 1200°C and soaked in simulated body fluid (SBF) for 28 days at 37°C; the mineralization process was followed by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction spectroscopy (XRD), and calcium (Ca) quantification analyses. The results showed that massive mineralization occurred exclusively at the surface of HA disks treated at 1200 °C and that nucleation of large aggregates of calcium phosphate (CaP) started specifically inside small concavities instead of on the planar surface of the disks. Regarding the effect of concavity diameter size on surface mineralization, it was observed that small concavities induce a 124- and 10-fold increased mineralization compared to concavities of large or medium size, respectively. The results of this study demonstrated that (i) in vitro surface mineralization of calcium phosphate ceramics with surface concavities starts preferentially within the concavities and not on the planar surface, and (ii) concavity size is an effective parameter to control the spatial position and extent of mineralization in vitro