109 research outputs found
Diferentes épocas de colheita, secagem e armazenamento na qualidade de grãos de trigo comum e duro
Evaluation of reduction in egg shedding of gastrointestinal nematodes in cattle following administration of anthelmintics
Xylella fastidiosa gene expression analysis by DNA microarrays
Xylella fastidiosa genome sequencing has generated valuable data by identifying genes acting either on metabolic pathways or in associated pathogenicity and virulence. Based on available information on these genes, new strategies for studying their expression patterns, such as microarray technology, were employed. A total of 2,600 primer pairs were synthesized and then used to generate fragments using the PCR technique. The arrays were hybridized against cDNAs labeled during reverse transcription reactions and which were obtained from bacteria grown under two different conditions (liquid XDM2 and liquid BCYE). All data were statistically analyzed to verify which genes were differentially expressed. In addition to exploring conditions for X. fastidiosa genome-wide transcriptome analysis, the present work observed the differential expression of several classes of genes (energy, protein, amino acid and nucleotide metabolism, transport, degradation of substances, toxins and hypothetical proteins, among others). The understanding of expressed genes in these two different media will be useful in comprehending the metabolic characteristics of X. fastidiosa, and in evaluating how important certain genes are for the functioning and survival of these bacteria in plants
Lembranças das abordagens na aquisição de LE/L2 de falantes e aprendizes e o fazer atual como professores
Early Planet Formation in Embedded Disks (eDisk). I. Overview of the Program and First Results
We present an overview of the Large Program, ``Early Planet Formation in
Embedded Disks (eDisk)'', conducted with the Atacama Large
Millimeter/submillimeter Array (ALMA). The ubiquitous detections of
substructures, particularly rings and gaps, in protoplanetary disks around T
Tauri stars raise the possibility that at least some planet formation may have
already started during the embedded stages of star formation. In order to
address exactly how and when planet formation is initiated, the program focuses
on searching for substructures in disks around 12 Class 0 and 7 Class I
protostars in nearby (200 pc) star-forming regions through 1.3 mm continuum
observations at a resolution of au (0.04"). The initial results show
that the continuum emission, mostly arising from dust disks around the sample
protostars, has relatively few distinctive substructures, such as rings and
spirals, in marked contrast to Class II disks. The dramatic difference may
suggest that substructures quickly develop in disks when the systems evolve
from protostars to Class II sources or alternatively that high optical depth of
the continuum emission could obscure internal structures. Kinematic information
obtained through CO isotopologue lines and other lines reveals the presence of
Keplerian disks around protostars, providing us with crucial physical
parameters, in particular, the dynamical mass of the central protostars. We
describe the background of the eDisk program, the sample selection and their
ALMA observations, the data reduction, and also highlight representative
first-look results.Comment: This is a publication of a series of eDisk ALMA large program
first-look paper
Comportamento do polipropileno em presença de monômeros trifuncionais no estado fundido e sua influência na morfologia
Study of the aging of fermented of yacon (Smallanthus sonchifolius) and sensory profile and acceptance
ESTUDO SOBRE OS RESULTADOS DA IRRIGAÇÃO EM COLOSTOMIZADOS SUBMETIDOS A UM PROCESSO DE TREINAMENTO SISTEMATIZADO
- …