ANTIPROLIFERATIVE ACTIVITY OF FLAVONOIDS ISOLATED FROM ECHINOPS GRACILIS O. HOFFM

Apigenin-7-O-(4′′-feruloyl)-β-D-glucopyranoside (1) were isolated from the methanol extract of aerial part of Echinops gracilis, together with apigenin-7-O-(4′′-trans-p-hydroxycinnamoyl)-β-D-glucopyranoside (2) and apigenin-7-O-β-D-glucopyranoside (3). Compound (1) previously displayed antioxidant and antibacterial activity. The present study aims at evaluating the antiproliferative potential of flavonoids, isolated from the aerial part of E. gracilis O. Hoffm. The effect of compounds (1), (2) and (3) on the viability of HeLa cells was determined by the method of 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay at different concentrations. The ability of compounds to induce the cell death was evaluated by using acridine orange/ethidium bromide (AO/EtBr) staining. Compound (1) induced an effective change in the cell viability of HeLa cells with IC50 concentration value of 27.36 µgmL-1. Induction of cell death alteration in cell morphology and cancer cell population was observed in cells treated with compound (1), which makes it behave as a potent synergistic antiproliferative agent against HeLa cells

An Antibacterial Isovaleronitrile Diglycoside From Detarium microcarpum Guill. Perr. (Fabaceae)

Feudjou WF, Mbock AM, Ouahouo MBW, et al. An Antibacterial Isovaleronitrile Diglycoside From Detarium microcarpum Guill. Perr. (Fabaceae). NATURAL PRODUCT COMMUNICATIONS. 2020;15(7): UNSP 1934578X20936939.A new isovaleronitrile diglycoside, named microcarposide (1), together with 6 known compounds: lupeol (2), betulinic acid (3), beta-sitosterol glucoside (4), methyl gallate (5), luteolin (6), and epicatechin (7), was isolated from the methanolic extract of the fruits of Detarium microcarpum Guill. Perr. The structures of the compounds were determined by extensive analysis of 1D- and 2D-H-1 and C-13 NMR spectroscopic data in conjunction with mass spectrometry and by comparison with data reported in the literature. Compound 1 was characterized as (2R)-2-[(6 ''-O-beta-L-rhamnopyranosyl-beta-D-glucopyranosyl)oxy]-3-methylbutanenitrile. Some of the isolated compounds were evaluated for their antibacterial activities against several microorganisms; only compound 1 was active against Salmonella typhi, Salmonella enteritidis, and Salmonella typhimurium with minimum inhibition concentration values of 153.4, 76.7, and 76.7 mu M, respectively