40 research outputs found

    Application of firefly luciferase assay for adenosine triphosphate (ATP) to antimicrobial drug sensitivity testing

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    The development of a rapid method for determining microbial susceptibilities to antibiotics using the firefly luciferase assay for adenosine triphosphate (ATP) is documented. The reduction of bacterial ATP by an antimicrobial agent was determined to be a valid measure of drug effect in most cases. The effect of 12 antibiotics on 8 different bacterial species gave a 94 percent correlation with the standard Kirby-Buer-Agar disc diffusion method. A 93 percent correlation was obtained when the ATP assay method was applied directly to 50 urine specimens from patients with urinary tract infections. Urine samples were centrifuged first to that bacterial pellets could be suspended in broth. No primary isolation or subculturing was required. Mixed cultures in which one species was predominant gave accurate results for the most abundant organism. Since the method is based on an increase in bacterial ATP with time, the presence of leukocytes did not interfere with the interpretation of results. Both the incubation procedure and the ATP assays are compatible with automation

    L'opera di Natalia Ginzburg

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    Two characteristics prevail in the works of Natalia Ginzburg: the tendency towards reduction and the tendency towards repetition. Reduction and repetition resemble each other; they are analogous, complementary; both are efficient and dramatic means to express a reality which is partial and incomplete and to underline the inherent obscure suffering which results from that reality. Although Natalia Ginzburg does not belong to any of the various literary trends (and, in particular, not to neo-realism which coincides chronologically with the beginning of her career), she interprets a sorry and limited world and bears sensitive witness to its loneliness, to its "male di vivere". In this sense, Natalia Ginzburg's works, although not in the mainstream of literary currents, touch the heart of modern literary sensibility.This is, in brief, the thrust of my thesis
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