Intrinsically Disordered C-Terminal Tails of \u3cem\u3eE. coli\u3c/em\u3e Single-Stranded DNA Binding Protein Regulate Cooperative Binding to Single-Stranded DNA

The homotetrameric Escherichia coli single-stranded DNA binding protein (SSB) plays a central role in DNA replication, repair and recombination. E. coli SSB can bind to long single-stranded DNA (ssDNA) in multiple binding modes using all four subunits [(SSB)65 mode] or only two subunits [(SSB)35 binding mode], with the binding mode preference regulated by salt concentration and SSB binding density. These binding modes display very different ssDNA binding properties with the (SSB)35 mode displaying highly cooperative binding to ssDNA. SSB tetramers also bind an array of partner proteins, recruiting them to their sites of action. This is achieved through interactions with the last 9 amino acids (acidic tip) of the intrinsically disordered linkers (IDLs) within the four C-terminal tails connected to the ssDNA binding domains. Here, we show that the amino acid composition and length of the IDL affects the ssDNA binding mode preferences of SSB protein. Surprisingly, the number of IDLs and the lengths of individual IDLs together with the acidic tip contribute to highly cooperative binding in the (SSB)35 binding mode. Hydrodynamic studies and atomistic simulations suggest that the E. coli SSB IDLs show a preference for forming an ensemble of globular conformations, whereas the IDL from Plasmodium falciparum SSB forms an ensemble of more extended random coils. The more globular conformations correlate with cooperative binding

Nonlinear wave interaction and spin models in the MHD regime

Here we consider the influence on the electron spin in the MHD regime. Recently developed models which include spin-velocity correlations are taken as a starting point. A theoretical argument is presented, suggesting that in the MHD regime a single fluid electron model with spin correlations is equivalent to a model with spin-up and spin-down electrons constituting different fluids, but where the spin-velocity correlations are omitted. Three wave interaction of 2 shear Alfven waves and a compressional Alfven wave is then taken as a model problem to evaluate the asserted equivalence. The theoretical argument turns out to be supported, as the predictions of the two models agree completely. Furthermore, the three wave coupling coefficients obey the Manley-Rowe relations, which give further support to the soundness of the models and the validity of the assumptions made in the derivation. Finally we point out that the proposed two-fluid model can be incorporated in standard Particle-In-Cell schemes with only minor modifications.Comment: 8 page

Reversal of particle flux in collisional-finite beta tokamak discharges

The mixed gradient method [Zhong et al. Phys. Rev. Lett. 111, 265001 (2013)] is adopted and effects of collisions and finite beta are included in the Weiland 9-equation fluid model. The particle flux and particle pinch, obtained using the Weiland anomalous transport fluid model, are compared with Tore Supra experimental results. Particle transport is also studied using predictive simulation data for an experimental advanced superconducting tokamak discharge in which neutral beam heating is utilized. The effects of collisions on particle transport are studied by turning collisions on and off in the Weiland model. It is found that the particle pinch region is related to the mode structure. The particle pinch region coincides with the region where the strong ballooning modes are present due to large gradients. The general properties of the fluid model are examined by finding regions where collisions can enhance the particle pinch

Zonal flow generation in collisionless trapped electron mode turbulence

In the present work the generation of zonal flows in collisionless trapped electron mode (TEM) turbulence is studied analytically. A reduced model for TEM turbulence is utilized based on an advanced fluid model for reactive drift waves. An analytical expression for the zonal flow growth rate is derived and compared with the linear TEM growth, and its scaling with plasma parameters is examined for typical tokamak parameter values.Comment: 20 pages, 4 figure

Comparison of the COBE FIRAS and DIRBE Calibrations

We compare the independent FIRAS and DIRBE observations from the COBE in the wavelength range 100-300 microns. This cross calibration provides checks of both data sets. The results show that the data sets are consistent within the estimated gain and offset uncertainties of the two instruments. They show the possibility of improving the gain and offset determination of DIRBE at 140 and 240 microns.Comment: Accepted for publication in the Astrophysical Journal 11 pages, plus 3 figures in separate postscript files. Figure 3 has three part

Perceived Differences in the Management of Mental Health Patients in Remote and Rural Australia and Strategies for Improvement: Findings from a National Qualitative Study of Emergency Clinicians

Introduction. We aimed to describe perceptions of Australian emergency clinicians of differences in management of mental health patients in rural and remote Australia compared with metropolitan hospitals, and what could be improved. Methods. Descriptive exploratory study using semi-structured telephone interviews of doctors and nurses in Australian emergency departments (EDs), stratified to represent states and territories and rural or metropolitan location. Content analysis of responses developed themes and sub-themes. Results. Of 39 doctors and 32 nurses responding to email invitation, 20 doctors and 16 nurses were interviewed. Major themes were resources/environment, staff and patient issues. Clinicians noted lack of access in rural areas to psychiatric support services, especially alcohol and drug services, limited referral options, and a lack of knowledge, understanding and acceptance of mental health issues. The clinicians suggested resource, education and guideline improvements, wanting better access to mental health experts in rural areas, better support networks and visiting specialist coverage, and educational courses tailored to the needs of rural clinicians. Conclusion. Clinicians managing mental health patients in rural and remote Australian EDs lack resources, support services and referral capacity, and access to appropriate education and training. Improvements would better enable access to support and referral services, and educational opportunities

Nonlinear Interactions Between Gravitational Radiation and Modified Alfven Modes in Astrophysical Dusty Plasmas

We present an investigation of nonlinear interactions between Gravitational Radiation and modified Alfv\'{e}n modes in astrophysical dusty plasmas. Assuming that stationary charged dust grains form neutralizing background in an electron-ion-dust plasma, we obtain the three wave coupling coefficients, and calculate the growth rates for parametrically coupled gravitational radiation and modified Alfv\'{e}n-Rao modes. The threshold value of the gravitational wave amplitude associated with convective stabilization is particularly small if the gravitational frequency is close to twice the modified Alfv\'en wave-frequency. The implication of our results to astrophysical dusty plasmas is discussed.Comment: A few typos corrected. Published in Phys. Rev.

Parametric excitation of plasma waves by gravitational radiation

We consider the parametric excitation of a Langmuir wave and an electromagnetic wave by gravitational radiation, in a thin plasma on a Minkowski background. We calculate the coupling coefficients starting from a kinetic description, and the growth rate of the instability is found. The Manley-Rowe relations are fulfilled only in the limit of a cold plasma. As a consequence, it is generally difficult to view the process quantum mechanically, i.e. as the decay of a graviton into a photon and a plasmon. Finally we discuss the relevance of our investigation to realistic physical situations.Comment: 5 pages, REVTe