10 research outputs found
Either Brain-Derived Neurotrophic Factor or Neurotrophin-3 Only Neurotrophin-Producing Grafts Promote Locomotor Recovery in Untrained Spinalized Cats
Leukemia inhibitory factor determines the growth status of injured adult sensory neurons
Leukemia inhibitory factor determines the growth status of injured adult sensory neurons
An Efficient Method for Dorsal Root Ganglia Neurons Purification with a One-Time Anti-Mitotic Reagent Treatment
Regulation of axonal regeneration by the level of function of the endogenous Nogo receptor antagonist LOTUS
Single-cell axotomy of cultured hippocampal neurons integrated in neuronal circuits
An understanding of the molecular mechanisms of axon regeneration after injury is key for the development of potential therapies. Single-cell axotomy of dissociated neurons enables the study of the intrinsic regenerative capacities of injured axons. This protocol describes how to perform single-cell axotomy on dissociated hippocampal neurons containing synapses. Furthermore, to axotomize hippocampal neurons integrated in neuronal circuits, we describe how to set up coculture with a few fluorescently labeled neurons. This approach allows axotomy of single cells in a complex neuronal network and the observation of morphological and molecular changes during axon regeneration. Thus, single-cell axotomy of mature neurons is a valuable tool for gaining insights into cell intrinsic axon regeneration and the plasticity of neuronal polarity of mature neurons. Dissociation of the hippocampus and plating of hippocampal neurons takes ∼2 h. Neurons are then left to grow for 2 weeks, during which time they integrate into neuronal circuits. Subsequent axotomy takes 10 min per neuron and further imaging takes 10 min per neuron