34 research outputs found
Caracterização de proteínas do plasma seminal e sua relação com parâmetros de qualidade do sêmen criopreservado em ovinos
Avaliação do modelo HYDRUS-1D na simulação do transporte de água e potássio em colunas preenchidas com solos tropicais
Morfologia, ultraestrutura e morfometria do tegumento da paca (Cuniculus paca Linnaeus, 1766) criada em cativeiro
Carboidratos não estruturais em tubérculos de batata recondicionados após o armazenamento sob diferentes temperaturas
High throughput pyrogenic carbon (biochar) characterisation and quantification by liquid chromatography
Characterisation and quantification of the carbonaceous polyaromatic structure of pyrogenic carbon (PyC) are of paramount importance to evaluate the role of PyC in soil carbon sequestration. A new method of rapid resolution liquid chromatography with UV diode array detection (RRLC-UV-DAD) was developed and is proposed to quantify benzene polycarboxylic acids (BPCAs), which are considered molecular markers of the aromatic structure of PyC. The proportion of aromatic carbon structures and the degree of aromatic condensation obtained from different PyC, i.e. different biomass pyrolysed at final temperature (450 °C), were quantified using the method of RRLC-UV-DAD. We showed that (i) the RRLC-UV-DAD analysis takes one quarter of the analysis time of conventional HPLC and half of the time of GC analysis, increasing BPCA analysis throughput; (ii) the optimised method showed high overall precision and low limits of quantification and (iii) PyC produced from different tropical biomass exhibited a wide range of BPCA yields and patterns