77 research outputs found
A polymerase III-like reinitiation mechanism is operating in regulation of histone expression in archaea
An archaeal histone gene from the hyperthermophile Pyrococcus furiosus containing four consecutive putative oligo-dT terminator sequences was used as a model system to investigate termination signals and the mechanism of termination in vitro. The archaeal RNA polymerase terminated with high efficiency at the first terminator at 90°C when it contained five to six T residues, at 80°C readthrough was significantly increased. A putative hairpin structure upstream of the first terminator had no effect on termination efficiency. Template competition experiments starting with RNA polymerase molecules engaged in ternary complexes revealed recycling of RNA polymerase from the terminator to the promoter of the same template. This facilitated reinitiation was dependent upon the presence of a terminator sequence suggest-ing that pausing at the terminator is required for recycling as in the RNA polymerase III system. Replacement of the sequences immediately down-stream of the oligo-dT terminator by an AT-rich segment improved termination efficiency. Both AT-rich and GC-rich downstream sequences seemed to impair the facilitated reinitiation pathway. Our data suggest that recycling is dependent on a subtle interplay of pausing of RNA polymerase at the ter-minator and RNA polymerase translocation beyond the oligo-dT termination signal that is dramatically affected by downstream sequences
Shell Corrections of Superheavy Nuclei in Self-Consistent Calculations
Shell corrections to the nuclear binding energy as a measure of shell effects
in superheavy nuclei are studied within the self-consistent Skyrme-Hartree-Fock
and Relativistic Mean-Field theories. Due to the presence of low-lying proton
continuum resulting in a free particle gas, special attention is paid to the
treatment of single-particle level density. To cure the pathological behavior
of shell correction around the particle threshold, the method based on the
Green's function approach has been adopted. It is demonstrated that for the
vast majority of Skyrme interactions commonly employed in nuclear structure
calculations, the strongest shell stabilization appears for Z=124, and 126, and
for N=184. On the other hand, in the relativistic approaches the strongest
spherical shell effect appears systematically for Z=120 and N=172. This
difference has probably its roots in the spin-orbit potential. We have also
shown that, in contrast to shell corrections which are fairly independent on
the force, macroscopic energies extracted from self-consistent calculations
strongly depend on the actual force parametrisation used. That is, the A and Z
dependence of mass surface when extrapolating to unknown superheavy nuclei is
prone to significant theoretical uncertainties.Comment: 14 pages REVTeX, 8 eps figures, submitted to Phys. Rev.
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Energetic particle influence on the Earth's atmosphere
This manuscript gives an up-to-date and comprehensive overview of the effects of energetic particle precipitation (EPP) onto the whole atmosphere, from the lower thermosphere/mesosphere through the stratosphere and troposphere, to the surface. The paper summarizes the different sources and energies of particles, principally
galactic cosmic rays (GCRs), solar energetic particles (SEPs) and energetic electron precipitation (EEP). All the proposed mechanisms by which EPP can affect the atmosphere
are discussed, including chemical changes in the upper atmosphere and lower thermosphere, chemistry-dynamics feedbacks, the global electric circuit and cloud formation. The role of energetic particles in Earthâs atmosphere is a multi-disciplinary problem that requires expertise from a range of scientific backgrounds. To assist with this synergy, summary tables are provided, which are intended to evaluate the level of current knowledge of the effects of energetic particles on processes in the entire atmosphere
Immunphaenotypisierung akuter lymphatischer Leukaemien: Diagnostische Aspekte und klinische Relevanz
Clinical significance of cytogenetic studies in childhood acute lymphoblastic leukemia: experience of the BFM trials
Immunophenotypic features of childhood and adult acute lymphoblastic leukemia (ALL): experience of the German Multicentre Trials ALL-BFM and GMALL
Die deutsch-polnischen Beziehungen - eine Interessen- und Wertegemeinschaft? Zur ersten deutsch-polnischen Elitestudie
SIGLEAvailable from TIB Hannover: F01B16 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman
Favorable outcome for children and adolescents with T-cell lymphoblastic lymphoma with an intensive ALL-type therapy without local radiotherapy
Precursor B-cell lymphoblastic lymphoma in childhood and adolescence: Clinical features, treatment, and results in trials NHL-BFM 86 and 90
Expression and regulation of c-kit receptor and response to stem cell factor in childhood malignant T-lymphoblastic cells
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