Cold winter temperatures condition the egg-hatching dynamics of a grape disease vector

The leafhopper Scaphoideus titanus is the vector of a major phytoplasma grapevine disease, Flavescence dorée. The vector’s distribution is in Eastern and Northern Europe, and its population dynamics varies as a function of vineyard latitude. We tested the hypothesis that hatching dynamics are cued by cold temperatures observed in winter. We exposed eggs from a natural population to simulated “cold” and “mild” winters and varied the exposure time at 5 °C from 0 to 63 days. We show that temperature cooling mainly affected the onset of hatching and is negatively correlated to the cold time exposure. The majority of hatchings occurred more quickly in cold rather than in mild winter simulated conditions, but there was no significant difference between the duration of hatching of eggs whatever the cold time exposure. In agreement with the Northern American origin of the vector, the diapause termination and thus the timing regulation of egg hatching require cold winters

Genetic variability in the diapause response of the burnet moth Zygaena trifolii (Lepidoptera: Zygaenidae).

A high degree of phenotypic variability was observed in the diapause response of the burnet moth Zygaena trifolii. In this study, we show that the observed variability is partly based on genetic differences between individuals. In a selection experiment, the larval instar at which diapause occurs was changed within six generations. Diapause instars were dependent on the time of pre-diapause development of larvae, which varied considerably between larvae. A heritability analysis indicates that a part of the variability in development time is based on additive genetic variance. The maintenance of genetic variability in the development time and the diapause response of Z. trifolii is discussed in the context of spatially and temporally changing selection pressure